2007
DOI: 10.1074/jbc.m700948200
|View full text |Cite
|
Sign up to set email alerts
|

Epitope Analysis of the Malaria Surface Antigen Pfs48/45 Identifies a Subdomain That Elicits Transmission Blocking Antibodies

Abstract: Pfs48/45, a member of a Plasmodium-specific protein family, displays conformation-dependent epitopes and is an important target for malaria transmission-blocking (TB) immunity. To design a recombinant Pfs48/45-based TB vaccine, we analyzed the conformational TB epitopes of Pfs48/45. The Pfs48/45 protein was found to consist of a C-terminal six-cysteine module recognized by anti-epitope I antibodies, a middle four-cysteine module recognized by anti-epitopes IIb and III, and an N-terminal module recognized by an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

3
69
0
1

Year Published

2008
2008
2017
2017

Publication Types

Select...
7
1

Relationship

2
6

Authors

Journals

citations
Cited by 70 publications
(73 citation statements)
references
References 25 publications
3
69
0
1
Order By: Relevance
“…Our results demonstrate that the yield of recombinant Pfs48/45 protein is significantly improved as compared with the recombinant 10C as described (15). A Pfs48/45 fragment of 10C cysteines retained the highest stability in terms of conformation and resistance to proteases and elicited high titers of functional TB antibodies.…”
mentioning
confidence: 59%
See 1 more Smart Citation
“…Our results demonstrate that the yield of recombinant Pfs48/45 protein is significantly improved as compared with the recombinant 10C as described (15). A Pfs48/45 fragment of 10C cysteines retained the highest stability in terms of conformation and resistance to proteases and elicited high titers of functional TB antibodies.…”
mentioning
confidence: 59%
“…Competition with nonconjugated anti-epitope I or III Pfs48/45 mAb was used as positive control and for quantification (data not A C B shown). Estimates based on unlabeled mAb added to the assay revealed concentrations of anti-epitope I and anti-epitope III antibodies in the sera of the M-Pfs10C-immunized mice between 20 and 100 g/ml, which is in the range in which TB activity can be expected (15). Briefly, a fixed amount of labeled mAb competed for Pfs48/45 binding with either test serum or a known concentration range of the same but unlabeled mAb.…”
Section: Purification and Primary Characterization Of M-pfs16c And M-mentioning
confidence: 99%
“…12 In each case, we targeted the antigenic region to which neutralizing antibodies have been predicted to bind. 13,14 Our deep-sequencing data, as well as previously published whole-genome sequencing data, show that these antigens are more conserved than preerythrocytic-and erythrocytic-stage antigens, but can still show significant antigenic diversity. 15 In this study, DNA was extracted from 329 dried blood spots of P. falciparum field isolates collected in previous studies across six countries, four in Africa and two in Asia (Supplemental Table 1).…”
mentioning
confidence: 91%
“…However, we did target regions predicted to be important for antibody binding. 13,14 Second, we used a set of samples collected previously for other purposes, representing different ages, clinical symptoms, and years collected. This may introduce biases for which we cannot account.…”
mentioning
confidence: 99%
“…SMFA Sera obtained from mice immunized with Pfs25-FhCMB were assayed for their TBA in SMFA as previously described. 37,38 Briefly, 30 µL of mouse or rabbit sera were mixed with 90 µL of naïve human serum and 150 µL of in vitro mature gametocyte culture of P. falciparum (NF54 line). This mixture was fed to Anopheles stephensi mosquitoes (Nijmegen strain) through a membrane-feeding apparatus.…”
Section: Pfs25-fhcmb Immunogenicity Studies In Mice and Rabbitsmentioning
confidence: 99%