Epitope Imprinting Approach to Monitor Diseases

Singh, Mona; Gupta, Namrata; Raghuwanshi, Richa

doi:10.4172/1747-0862.1000270

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…2). In this line exposed peptides (epitopes) [20,21] or protein subunits [22] have been used as the template in the synthesis of MIPs, which recognize both the epitope and the holo-protein. The concept of using an exposed peptide sequence as the target in the MIPsynthesis -the epitope imprinting approach-has been extended to artificial peptide tags of engineered proteins [23], sugars of glycoproteins [24] and even to chemical labels of macromolecules [25].…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Molecularly imprinted polymer-based electrochemical sensors for biopolymers

Scheller

Zhang

Yarman

et al. 2019

Current Opinion in Electrochemistry

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Electrochemical synthesis and signal generation dominate among the almost 1200 papers published annually on protein imprinted polymers. Such polymers can be easily prepared directly on the electrode surface and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture the molecularly imprinted polymer (MIP) layer represents only one "separation plate", thus the selectivity does not reach the values of "bulk" measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an "overall apparent" signal which may include non-specific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.

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Section: A N U S C R I P Tmentioning

confidence: 99%

Molecularly imprinted polymer-based electrochemical sensors for biopolymers

Scheller

Zhang

Yarman

et al. 2019

Current Opinion in Electrochemistry

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“…epitope imprinting (Gupta et al, 2016;Kushwaha et al, 2019;Singh et al, 2017) and surface imprinting (Li et al, 2018;Luo et al, 2017;Mitchell et al, 2019). Surface imprinting has nowadays emerged as the most commonly applied method for glycoprotein imprinting, as it overcomes diffusion limitations caused by the dimensions of proteins.…”

mentioning

confidence: 99%

Molecularly imprinted materials for biomedical sensing

2021

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The development of medicine during the last decades was mainly responsible for the increase in population life expectancy. These advances were achieved owing to contributions by several science areas including biochemistry, chemistry, pharmacology and engineering (Blyakhman et al., 2017; Feldman, 2020). The development of new diagnostic tools is among the most relevant subjects in this context, since disease detection at premature stages enables avoiding evasive, complex and expansive treatments and principally spare lives, especially when detecting contagious diseases (Steele et al., 2016). Consequently, rapidly responding, specific, sensitive, robust and ideally non-invasive diagnostic tools are essential for tracking and preventing infections (Sabherwal et al., 2016; Sharafeldin et al., 2018). The development of advanced diagnostic tools is challenged by factors such as the type of target analyte, the variety and complexity of biological samples (i.e. tissues, biofluids, etc.), and the frequently pronouncedly low concentration levels of the analyte, which may directly or in combination affect the achievable selectivity and sensitivity (Ranadive et al., 2017). Despite recent advances in diagnostic tools, several issues remain unaddressed. Most diagnostic tests rely on the use of sophisticated biological receptors, such as antibodies, enzymes, DNA, serving as biochemical or chemical recognition elements. Due to their nature, these reagents may be subject to poor stability, limited availability and/or reproducibility during manufacturing/processing and contaminations affecting the analysis (Kilic et al., 2020). Consequently, synthetic receptor/molecular recognition materials such as MIPs are a promising alternative to overcome or minimize some of these limitations (Denmark et al., 2020; Qi et al., 2019). Using molecular imprinting strategies, selectivity for a specific target species is entailed into an inorganic material, which may then be added to biodiagnostic and biomedical devices instead of natural receptor motifs, as discussed in the remainder of this review.

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