Epitope imprinting of Mycobacterium leprae bacteria via molecularly imprinted nanoparticles using multiple monomers approach

Kushwaha, Archana; Srivastava, J.P.; Singh, Ambareesh Kumar; Anand, Richa; Raghuwanshi, Richa; Rai, Tulika; Singh, Meenakshi

doi:10.1016/j.bios.2019.111698

Cited by 53 publications

(34 citation statements)

References 22 publications

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“…IF = 1.9 was also followed to prepare MIP-coated QCM sensors for detection of Mycobacterium leprae, attaining an order of magnitude lower LOD (0.161 nM) (109). The epitope selected by the authors had previously been shown to stimulate interferon- secretion by T cells from infected patients, thus being identified as a natural antigen (110).…”

Section: Terminusmentioning

confidence: 99%

“…In a combination of techniques, 3-sulfopropyl methacrylate/benzyl methacrylate MINPs were first produced, followed by electropolymerization of 4-aminothiophenol to bind the particles together, coating a QCM sensor (109). The new MIP achieved remarkable results, with an IF of 8.28, a selectivity coefficient of 4.34 against the same peptide with just two amino acids switching positions in the sequence, and a subnanomolar LOD.…”

Section: Other Organic Monomersmentioning

confidence: 99%

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Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules

et al. 2021

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Section: Terminusmentioning

confidence: 99%

Section: Other Organic Monomersmentioning

confidence: 99%

Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules

et al. 2021

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“…However, the design and synthesis of MIPs selective for proteins are challenging due to the conformational variations of the proteins during the imprinting process. To overcome this drawback, several strategies have been proposed such as epitope imprinting (Gupta et al, 2016; Kushwaha et al, 2019; Singh et al, 2017) and surface imprinting (Li et al, 2018; Luo et al, 2017; Mitchell et al, 2019). Surface imprinting has nowadays emerged as the most commonly applied method for glycoprotein imprinting, as it overcomes diffusion limitations caused by the dimensions of proteins.…”

Section: Applications In Biomedical Scenariosmentioning

confidence: 99%

“…epitope imprinting (Gupta et al, 2016;Kushwaha et al, 2019;Singh et al, 2017) and surface imprinting (Li et al, 2018;Luo et al, 2017;Mitchell et al, 2019). Surface imprinting has nowadays emerged as the most commonly applied method for glycoprotein imprinting, as it overcomes diffusion limitations caused by the dimensions of proteins.…”

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confidence: 99%

Molecularly imprinted materials for biomedical sensing

2021

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The development of medicine during the last decades was mainly responsible for the increase in population life expectancy. These advances were achieved owing to contributions by several science areas including biochemistry, chemistry, pharmacology and engineering (Blyakhman et al., 2017; Feldman, 2020). The development of new diagnostic tools is among the most relevant subjects in this context, since disease detection at premature stages enables avoiding evasive, complex and expansive treatments and principally spare lives, especially when detecting contagious diseases (Steele et al., 2016). Consequently, rapidly responding, specific, sensitive, robust and ideally non-invasive diagnostic tools are essential for tracking and preventing infections (Sabherwal et al., 2016; Sharafeldin et al., 2018). The development of advanced diagnostic tools is challenged by factors such as the type of target analyte, the variety and complexity of biological samples (i.e. tissues, biofluids, etc.), and the frequently pronouncedly low concentration levels of the analyte, which may directly or in combination affect the achievable selectivity and sensitivity (Ranadive et al., 2017). Despite recent advances in diagnostic tools, several issues remain unaddressed. Most diagnostic tests rely on the use of sophisticated biological receptors, such as antibodies, enzymes, DNA, serving as biochemical or chemical recognition elements. Due to their nature, these reagents may be subject to poor stability, limited availability and/or reproducibility during manufacturing/processing and contaminations affecting the analysis (Kilic et al., 2020). Consequently, synthetic receptor/molecular recognition materials such as MIPs are a promising alternative to overcome or minimize some of these limitations (Denmark et al., 2020; Qi et al., 2019). Using molecular imprinting strategies, selectivity for a specific target species is entailed into an inorganic material, which may then be added to biodiagnostic and biomedical devices instead of natural receptor motifs, as discussed in the remainder of this review.

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“…Showing a diverse molecular structure, aminopenicillins encompass a broad range of structural derivatives of ampicillin (which itself is an amino benzylpenicillin) ( Figure 1 ) [ 28 , 29 ]. The multiple functionalities within the molecules gives rise to the possibility of different binding opportunities, meaning the selection of functional monomers/crosslinkers is not as definitive as for simpler mono-functional molecules [ 30 ]. Therefore, a degree of rational design is required, analyzing the potential binding groups and selecting compositions that not only interact with one functional, but with multiple [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Sensing of Amoxicillin Facilitated by Molecularly Imprinted Polymers

et al. 2021

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The scope of the presented research orientates itself towards the development of a Molecularly Imprinted Polymer (MIP)-based dye displacement assay for the colorimetric detection of the antibiotic amoxicillin in aqueous medium. With this in mind, the initial development of an MIP capable of such a task sets focus on monolithic bulk polymerization to assess monomer/crosslinker combinations that have potential towards the binding of amoxicillin. The best performing composition (based on specificity and binding capacity) is utilized in the synthesis of MIP particles by emulsion polymerization, yielding particles that prove to be more homogenous in size and morphology compared to that of the crushed monolithic MIP, which is an essential trait when it comes to the accuracy of the resulting assay. The specificity and selectivity of the emulsion MIP proceeds to be highlighted, demonstrating a higher affinity towards amoxicillin compared to other compounds of the aminopenicillin class (ampicillin and cloxacillin). Conversion of the polymeric receptor is then undertaken, identifying a suitable dye for the displacement assay by means of binding experiments with malachite green, crystal violet, and mordant orange. Once identified, the optimal dye is then loaded onto the synthetic receptor, and the displaceability of the dye deduced by means of a dose response experiment. Alongside the sensitivity, the selectivity of the assay is scrutinized against cloxacillin and ampicillin. Yielding a dye displacement assay that can be used (semi-)quantitatively in a rapid manner.

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Epitope imprinting of Mycobacterium leprae bacteria via molecularly imprinted nanoparticles using multiple monomers approach

Cited by 53 publications

References 22 publications

Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules

Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules

Molecularly imprinted materials for biomedical sensing

Colorimetric Sensing of Amoxicillin Facilitated by Molecularly Imprinted Polymers

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