Epitope Mapping

Morris, Glenn E.

doi:10.1007/978-1-60327-375-6_38

Cited by 3 publications

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies

Morris¹,

Mân²,

Sewry³

2010

Methods in Molecular Biology

View full text Add to dashboard Cite

Several molecular approaches to Duchenne muscular dystrophy (DMD) therapy are at or near the point of clinical trial and usually involve attempts to replace the missing dystrophin protein. Although improved muscle function is the ultimate measure of success, assessment of dystrophin levels after therapy is essential to determine whether any improved function is a direct consequence of the treatment or, in the absence of improved function, to determine whether new dystrophin is present, though ineffective. The choice of a monoclonal antibody (mAb) to distinguish successful therapy from naturally occurring "revertant" fibres depends on which dystrophin exons are deleted in the DMD patient. Over the past 20 years, we have produced over 150 "exon-specific" mAbs, mapped them to different regions of dystrophin and made them available through the MDA Monoclonal Antibody Resource for research and for clinical trials tailored to individual patients. In this protocol, we describe the use of these mAb to monitor DMD gene therapy.

show abstract

Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies

Morris¹,

Mân²,

Sewry³

2010

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

Linear epitope mapping by native mass spectrometry

DeFelippis

Huang

2009

Analytical Biochemistry

View full text Add to dashboard Cite

Characterizing Antibodies

Weis-Garcia¹,

Carnahan²

2017

Cold Spring Harb Protoc

View full text Add to dashboard Cite

Perhaps because they are such commonly used tools, many researchers view antibodies one-dimensionally: Antibody Y binds antigen X. Although few techniques require a comprehensive understanding of any particular antibody’s characteristics, well-executed experiments do require a basic appreciation of what is known and, equally as important, what is not known about the antibody being used. Ignorance of the relevant antibody characteristics critical for a particular assay can easily lead to loss of precious resources (time, money, and limiting amounts of sample) and, in worst-case scenarios, erroneous conclusions. Here, we describe various antibody characteristics to provide a more well-rounded perspective of these critical reagents. With this information, it will be easier to make informed decisions on how best to choose and use the available antibodies, as well as knowing when it is essential and how to determine a particular as yet-undefined characteristic.

show abstract

Epitope Mapping

Cited by 3 publications

References 99 publications

Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies

Monitoring Duchenne Muscular Dystrophy Gene Therapy with Epitope-Specific Monoclonal Antibodies

Linear epitope mapping by native mass spectrometry

Characterizing Antibodies

Contact Info

Product

Resources

About