Epitope mapping of recombinant human gamma interferon using monoclonal antibodies

Favre, Catherine; Wijdenes, John; Cabrillat, H.; Djossou, Odile; Banchereau, Jacques; Vries, J E de

doi:10.1016/0161-5890(89)90015-1

Cited by 43 publications

(29 citation statements)

References 33 publications

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“…Among the four MAbs tested, only B22 prevent the binding between IFN and heparan sulfate (Table II). This MAb recognizes the last 15 amino-acids (carboxy-terminal side) of the IFN-'y molecule, whereas B27, 32, and 35 define other domains (36). These data demonstrate that the carboxy-terminal part of IFN-,y is involved in its binding to heparan sulfate.…”

Section: Methodsmentioning

confidence: 77%

High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

Lortat‐Jacob¹,

Kleinman²,

Grimaud³

1991

J. Clin. Invest.

114

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Recently it was demonstrated that growth factors are bound to the extracellular matrix, and can regulate cell behavior. Using three different types of binding assays, we have examined the interaction of interferon-t with a basement membrane produced by the Engelbreth-Holm-Swarm tumor. Basement membrane was found to bind interferon-y in both a time-and concentration-dependent manner. Equilibrium binding analysis revealed a high-affinity site with a dissociation constant of 1.5 10-' M and a maximum binding capacity of 1.6 10' sites/mm2 ofbasement membrane. Competition studies show that the binding is inhibited by heparan sulfate, suggesting that basement membrane-heparan sulfate proteoglycan could be the binding site. This interaction was clearly confirmed by native polyacrylamide gel electrophoresis and dot-blot analysis with purified basement membrane molecules. Furthermore, the carboxy-terminal part of the interferon-'y molecule contains an amino acid cluster, very closely related to a consensus sequence, present in more than 20 proteins known to bind sulfated glycosaminoglycans such as heparin. These data demonstrate a possible role of extracellular matrix components in storing cytokines and in modulating the cellular response to such factors. (J. Clin. Invest. 1991. 87:878-883.).

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Section: Methodsmentioning

confidence: 77%

High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

Lortat‐Jacob¹,

Kleinman²,

Grimaud³

1991

J. Clin. Invest.

114

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“…Human IFN-g and human and murine IL-2 were purchased from R&D Systems and used at 5 ng/ml for culture or 50 ng/ml for stimulation. Anti-IFN-g antibody B27, described previously, (35) was used at 10 mg/ml.…”

Section: Antibodies and Cytokines For Cell Culturementioning

confidence: 99%

IL-27 and IFN-αSignal via Stat1 and Stat3 and Induce T-Bet and IL-12Rβ2 in Naive T Cells

Hibbert¹,

Pflanz²,

Malefyt³

et al. 2003

Journal of Interferon & Cytokine Research

291

261

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Interleukin-27 (IL-27) supports proliferation of naive CD4(+) T cells and enhances interferon-gamma (IFN-gamma) production by activated T cells and natural killer (NK) cells. We report here that IL-27 induces Stat1 and Stat3 phosphorylation and activation in human and murine cell lines and primary human T cells. IL-27 also induces T-Bet, a Stat1-dependent gene crucial to Th1 cell commitment. Similarly, IFN-alpha activates Stat1 and Stat3 and T-Bet expression in naive T cells. Induction of T-Bet results in upregulation of IL-12Rbeta2 on naive T cells, which is essential for responsiveness to IL-12 and differentiation to a Th1 phenotype. Both IL-27 and IFN-alpha induce expression of IL-12Rbeta2 in T cells. In contrast, IFN-gamma, which activates Stat1 but not Stat3, induces expression of T-Bet but not IL-12Rbeta2 in naive T cells. We propose that IL-27 and IFN-alpha are important for early Th1 commitment and act upstream of IL-12 and IFN-gamma in this pathway.

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“…Monoclonal antibodies were developed by Favre et al [16] and used by Abrams et al [17] to develop a sensitive quantitative ELISA assay for the determination of IFN-γ in fluids. Monoclonal antibody 35 was used as the capture antibody.…”

Section: Methodsmentioning

confidence: 99%

IgG Antibody Subclasses, Tumor Necrosis Factor and IFN-γ Levels in Patients with Type II Lepra Reaction on Thalidomide Treatment

Partida-Sánchez

Favila-Castillo

Pedraza-Sánchez

et al. 1998

Int Arch Allergy Immunol

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A group of 9 Mexican lepromatous leprosy patients was studied at the beginning of a type II reaction (erythema nodosum leprosum, ENL) and after 1 or 2 months of thalidomide treatment. ENL patients at the onset of the reaction had slightly higher amounts of anti-Mycobacterium leprae IgG1 and IgG2 antibodies, compared to similar lepromatous patients that did not develop ENL. Neither these antibody levels nor IgM and the other IgG subclasses were importantly modified after thalidomide treatment. Serum TNF was significantly higher in the patients that developed ENL compared to those that did not develop the reaction. TNF levels were slightly decreased after 1 month of thalidomide treatment and significantly decreased after 2 months of treatment. Serum IFN-γ was significantly lower in patients at the onset of ENL and was increased after 1 and 2 months of thalidomide treatment.

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Epitope mapping of recombinant human gamma interferon using monoclonal antibodies

Cited by 43 publications

References 33 publications

High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

High-affinity binding of interferon-gamma to a basement membrane complex (matrigel).

IL-27 and IFN-αSignal via Stat1 and Stat3 and Induce T-Bet and IL-12Rβ2 in Naive T Cells

IgG Antibody Subclasses, Tumor Necrosis Factor and IFN-γ Levels in Patients with Type II Lepra Reaction on Thalidomide Treatment

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