Epitope Specificity of a Monoclonal Antibody Generated against the Dissociated CFA/I Fimbriae of Enterotoxigenic <i>Escherichia coli</i>

Luna, Maria das Graças de; Rudin, Anna; Vinhas, Solange Alves; Almeida, Darcy Fontoura de; Ferreira, Luís Carlos de Souza

doi:10.1111/j.1348-0421.1998.tb02293.x

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Indirect immunofluorescence tests were carried out with fixed pRECFA‐transfected BHK‐21 cells incubated for 1 h at 37°C with MAb DL6, specific for a gD linear epitope (amino acids 272–279), or MAb 84, specific for a CFA/I linear epitope (amino acids 39–43) [23]. Cells were washed with PBS and incubated for 1 h at room temperature with goat anti‐mouse IgG FITC‐conjugated secondary antibody (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenicEscherichia coliDNA vaccine

Lásaro

Alves

Botosso

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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The antibody-inducing properties of a bacterial/viral bivalent DNA vaccine (pRECFA), expressing a peptide composed of N-and C-terminal amino acid sequences of the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) fused with an inner segment encoding the major structural subunit of enterotoxigenic Escherichia coli (ETEC) CFA/I fimbriae (CFA/I), was evaluated in BALB/c mice following intramuscular immunization. The bivalent pRECFA vaccine elicited serum antibody responses, belonging mainly to the IgG2a subclass, against both CFA/I and HSV gD proteins. pRECFA-elicited antibody responses cross-reacted with homologous and heterologous ETEC fimbrial antigens as well as with type 1 and type 2 HSV gD proteins, which could bind and inactivate intact HSV-2 particles. On the other hand, CFA/I-specific antibodies could bind but did not neutralize the adhesive functions of the bacterial CFA/I fimbriae. In spite of the functional restriction of the antibodies targeting the bacterial antigen, the present evidence suggests that fusion of heterologous peptides to the HSV gD protein represents an alternative for the design of bivalent DNA vaccines able to elicit serum antibody responses.

show abstract

Section: Methodsmentioning

confidence: 99%

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenicEscherichia coliDNA vaccine

Lásaro

Alves

Botosso

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Indirect immuno£uorescence tests were carried out with ¢xed pRECFA-transfected BHK-21 cells incubated for 1 h at 37 ‡C with MAb DL6, speci¢c for a gD linear epitope (amino acids 272^279), or MAb 84, speci¢c for a CFA/I linear epitope (amino acids 39^43) [23]. Cells were washed with PBS and incubated for 1 h at room temperature with goat anti-mouse IgG FITC-conjugated secondary antibody (Sigma).…”

Section: Immuno£uorescence Analysismentioning

confidence: 99%

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenic Escherichia coli DNA vaccine

LASARO¹

2003

FEMS Immunology and Medical Microbiology

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The antibody-inducing properties of a bacterial/viral bivalent DNA vaccine (pRECFA), expressing a peptide composed of N- and C-terminal amino acid sequences of the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) fused with an inner segment encoding the major structural subunit of enterotoxigenic Escherichia coli (ETEC) CFA/I fimbriae (CFA/I), was evaluated in BALB/c mice following intramuscular immunization. The bivalent pRECFA vaccine elicited serum antibody responses, belonging mainly to the IgG2a subclass, against both CFA/I and HSV gD proteins. pRECFA-elicited antibody responses cross-reacted with homologous and heterologous ETEC fimbrial antigens as well as with type 1 and type 2 HSV gD proteins, which could bind and inactivate intact HSV-2 particles. On the other hand, CFA/I-specific antibodies could bind but did not neutralize the adhesive functions of the bacterial CFA/I fimbriae. In spite of the functional restriction of the antibodies targeting the bacterial antigen, the present evidence suggests that fusion of heterologous peptides to the HSV gD protein represents an alternative for the design of bivalent DNA vaccines able to elicit serum antibody responses.

show abstract

Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

2014

Bacterial Adhesion to Animal Cells and Tissues

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Epitope Specificity of a Monoclonal Antibody Generated against the Dissociated CFA/I Fimbriae of Enterotoxigenic Escherichia coli

Cited by 3 publications

References 15 publications

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenicEscherichia coliDNA vaccine

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenicEscherichia coliDNA vaccine

Antibody-inducing properties of a prototype bivalent herpes simplex virus/enterotoxigenic Escherichia coli DNA vaccine

Adhesins, Receptors, and Target Substrata Involved in the Adhesion of Pathogenic Bacteria to Host Cells and Tissues

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