Epizootology and experimental infection of Yokose virus in bats

Watanabe, Shumpei; Omatsu, Tsutomu; Miranda, Mary Elizabeth; Masangkay, Joseph S.; Ueda, Naoya; Endo, Mitsugi; Kato, Kentaro; Tohya, Yukinobu; Yoshikawa, Yasuhiro; Akashi, Hiroomi

doi:10.1016/j.cimid.2008.07.008

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…This route has been used in experimental protocols to inoculate mice with DENV 39 and bats with DENV and other flaviviruses. 40,41 However, no evidence of viremia, antigenemia or serological immune response was found after EI-1 or EI-2. Analysis of spleen tissue, an organ found to be infected in humans 42 and bats 15 also failed to show evidence of DENV replication.…”

Section: Discussionmentioning

confidence: 96%

“…Our results are also in agreement with previous reports of experimental inoculation of 2 species of North American bats with WNV or Australian black flying foxes with JEV, two flavivirus closely related to DENV, and one species of Asian bats with yokose virus, a flavivirus distantly related to DENV. 37,38,40 In contrast, low to relatively high levels of susceptibility were observed in three species of insectivorous bats experimentally inoculated with several strains of JEV or St. Louis encephalitis virus (SLEV). 50 One limitation of this work is that field-collected bats were tested only for active DENV infection and not for anti-DENV antibodies that may have prevented infection, before the EIs.…”

Section: Discussionmentioning

confidence: 98%

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Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Cabrera-Romo¹,

Recio-Tótoro²,

Alcalá³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Dengue is the most important mosquito-borne viral disease to humans. Bats are potential reservoirs for flaviviruses, including dengue virus (DENV). In this work, Artibeus jamaicensis bats were inoculated with two serotypes of DENV using different routes. For experimental inoculations (EI) 1 and 2, bats were inoculated subcutaneously or intraperitoneally with DENV-4; for EI-3 bats were inoculated intraperitoneally with DENV-1. Mock inoculated bats were kept as controls. In EI-4, bats were bitten by Aedes aegypti mosquitoes infected with DENV-1 or 4. Reverse transcription-polymerase chain reaction assays in plasma and spleen tissue collected from Day 1 to Days 9-17 after inoculation failed to reveal the presence of viral RNA in any of the samples. No evidence of circulating NS1 or specific anti-DENV IgG was detected in the plasma of the inoculated bats. These results indicate that A. jamaicensis bats are incapable of sustaining dengue virus replication and are unlikely to act as reservoirs for this virus.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Cabrera-Romo¹,

Recio-Tótoro²,

Alcalá³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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“…The bats were euthanized under sedation as described previously [5]. Samples of approximately 100 mg of each spleen were used for DNA extraction using a QIAamp DNA mini kit (QIAGEN), according to the manufacturer's instructions.…”

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confidence: 99%

Detection of a new bat gammaherpesvirus in the Philippines

et al. 2009

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A new bat herpesvirus was detected in the spleen of an insectivorous bat (Hipposideros diadema, family Hipposideridae) collected on Panay Island, the Philippines. PCR analyses were performed using COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) targeting the herpesvirus DNA polymerase (DPOL) gene. Although we obtained PCR products with CODEHOPs, direct sequencing using the primers was not possible because of high degree of degeneracy. Direct sequencing technology developed in our rapid determination system of viral RNA sequences (RDV) was applied in this study, and a partial DPOL nucleotide sequence was determined. In addition, a partial gB gene nucleotide sequence was also determined using the same strategy. We connected the partial gB and DPOL sequences with long-distance PCR, and a 3741-bp nucleotide fragment, including the 3' part of the gB gene and the 5' part of the DPOL gene, was finally determined. Phylogenetic analysis showed that the sequence was novel and most similar to those of the subfamily Gammaherpesvirinae.

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“…Although the strains were isolated from different bat species, Miniopterus fuliginosus (Miniopterus, Miniopteridae) and Myotis daubentonii (Myotis, Vespertilioninae), both species belong to the suborder Microchiroptera of the order Chiroptera [18]. The isolation of YOKVs from microbats on two separate occasions suggests that microbats may be suitable hosts for YOKV, although the YOKV replicates poorly in the fruit bat [9].…”

Section: Discussionmentioning

confidence: 99%

“…The above results suggested that this new member of flavivirus is genetically related to yellow fever virus. A serological survey of YOKV in the Philippines and Malaysia showed that the YOKV antibody was detected in the serum of bats ( Rousettus leschenaultii ), suggesting that the distribution of YOKV was not limited to Yokosuka Island, Japan [9] (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the Yokose virus, XYBX1332, isolated from bats (Myotis daubentonii) in China

Feng

Ren

et al. 2019

Virol J

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BackgroundYokose virus was first isolated from bats (Miniopterus fuliginosus) collected in Yokosuka, Japan, in 1971, and is a new member of the family Flaviviridae, genus Flavivirus. In this study, we isolated a Yokose virus from a serum sample of Myotis daubentonii (order Chiroptera, family Vespertilionidae) collected in Yunnan province, China in 2013.MethodsThe serum specimens of bat were used to inoculate in BHK-21 and Vero E6 cells for virus isolation. Then the viral complete genome sequence was obtained and was used for phylogenetic analysis performed by BEAST software package.ResultsThe virus was shown to have cytopathic effects in mammalian cells (BHK-21 and Vero E6). Genome sequencing indicated that it has a single open reading frame (ORF), with a genome of 10,785 nucleotides in total. Phylogenetic analysis of the viral genome suggests that XYBX1332 is a Yokose virus (YOKV) of the genus Flavivirus. Nucleotide and amino acid homology levels of the ORF of XYBX1332 and Oita-36, the original strain of YOKV, were 72 and 82%, respectively. The ORFs of XYBX1332 and Oita-36 encode 3422 and 3425 amino acids, respectively. In addition, the non-coding regions (5′- and 3′-untranslated regions [UTRs]) of these two strains differ in length and the homology of the 5′- and 3′-UTRs was 81.5 and 78.3%, respectively.ConclusionThe isolation of YOKV (XYBX1332) from inland China thousands of kilometers from Yokosuka, Japan, suggests that the geographical distribution of YOKV is not limited to the islands of Japan and that it can also exist in the inland areas of Asia. However, there are large differences between the Chinese and Japanese YOKV strains in viral genome.

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Epizootology and experimental infection of Yokose virus in bats

Cited by 9 publications

References 28 publications

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Experimental Inoculation of Artibeus jamaicensis Bats with Dengue Virus Serotypes 1 or 4 Showed No Evidence of Sustained Replication

Detection of a new bat gammaherpesvirus in the Philippines

Genetic diversity of the Yokose virus, XYBX1332, isolated from bats (Myotis daubentonii) in China

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