EPR and Mössbauer Characteristics of Aqueous Solutions of 57Fe-Dinitrosyl Iron Complexes with Glutathione and Hydroxyl Ligands

Prusakov, Valery E.; Maksimov, Yury V.; Burbaev, D. Sh.; Сереженков, В. А.; Borodulin, R. R.; Tkachev, N. A.; Mikoyan, V. D.; Vanin, Anatoly F.

doi:10.1007/s00723-019-1112-8

Cited by 7 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reasons of contradictory functions of NO include synthesis by different NO-synthases as a main source of NO [6,14], presence of nitroreductase component of NO cycle [4,9], and significant number of depot for NO which interact with iron-containing complexes (e.g. haem structures) with thiols and other compounds [10,31,32].…”

Section: Study Results and Their Discussionmentioning

confidence: 99%

Application of EPR spectroscopy to study the content of NO and copper in the frontal lobes, hippocampus and liver of rats after brain ischemia

et al. 2022

View full text Add to dashboard Cite

Electron paramagnetic resonance (EPR) spectroscopy was used to record the content of nitric oxide (NO) and copper in brain tissues (frontal lobes and hippocampus) and liver of healthy rats and rats after ischemia modeling. Ischemia was simulated by ligation of the carotid arteries, followed by taking 3 ml of blood from the common carotid artery. Signals from triple complexes (DETC) were recorded by EPR spectroscopy of complexes (DETC)2-Fe2+-(NO) and Cu(DETC)2. Based on direct measurements by EPR spectroscopy, it was shown that a day after the modeling of ischemia, NO production in the hippocampus decreases by an average of 30% and there is a tendency to decrease NO in the frontal lobes and liver. The copper content decreased by an average of 3 times in the frontal lobes and the hippocampus by an average of 20% a day after ischemia modeling, and a tendency to decrease was noted in the liver. Thus, brain hypoxia is accompanied not only by a decrease in NO production, but also by signs of weakening of the antioxidant system in the hippocampus and frontal lobes, which further worsens the functional state of the homeostasis system. Keywords: electron paramagnetic resonance, spin trap, nitric oxide, cerebral ischemia, frontal lobes, hippocampus.

show abstract