EPR Detection of Reactive Oxygen Species in Hemolymph of Galleria mellonella and Dendrolimus superans sibiricus (Lepidoptera) Larvae

Slepneva, Irina A.; Глупов, В. В.; Sergeeva, Svetlana V.; Khramtsov, Valery V.

doi:10.1006/bbrc.1999.1504

Cited by 60 publications

(42 citation statements)

References 17 publications

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“…NADPH oxidase and NO synthase pathways have been observed in the selfdefense system of Mytilus galloprovincialis, 18) and production of ROS has also been detected in hemocytes; evidence of both O 2 À 19) and its dismutation product, H 2 O 2 , has been found in plasmatocytes of Drosophila melanogaster larvae 20) and Galleria melonella. 21) This protein might play an important role in the defense system of the silkworm against BmNPV. It is quite possible that it might function to destroy the virus in other insects or other animals.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice ofBombyx mori

Selot

Kumar

Shukla

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice ofBombyx mori

Selot

Kumar

Shukla

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…Key features involved in innate immunity are found to be homologous in insects and vertebrates [12,13]. The insect cellular processes such as phagocytosis [14], production of reactive oxygen species [15] and anti-microbial peptides, bear strong similarity to those produced by phagocytes of mammals, giving evidence of an ancient immune system found in the ancestor of all bilaterian animals. Most immunological data for insects have been reported from Drosophila melanogaster [16] however in the Greater Wax moth Galleria mellonella, various immunorelevant protein molecules have been described and this system is being increasingly used as a model for assessing the virulence of a range of microorganisms [17,18].…”

Section: Introductionmentioning

confidence: 99%

Correlation between Gliotoxin Production and Virulence of Aspergillus fumigatus in Galleria mellonella

et al. 2004

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Aspergillus fumigatus is a pathogenic fungus capable of causing both allergic lung disease and invasive aspergillosis, a serious, life-threatening condition in neutropenic patients. Aspergilli express an array of mycotoxins and enzymes which may facilitate fungal colonisation of host tissue. In this study we investigated the possibility of using the insect, Galleria mellonella, for in vivo pathogenicity testing of Aspergillus species. Four clinical isolates of Aspergillus fumigatus and a single strain of Aspergillus niger were characterised for catalase and elastase activity and for the production of gliotoxin. Gliotoxin is an immunosuppressive agent previously implicated in assisting tissue penetration. Results illustrated a strain dependent difference in elastase activity but no significant difference in catalase activity. Gliotoxin production was detected in vitro and in vivo by Reversed Phase-High Performance Liquid Chromatography, with highest amounts being produced by A. fumigatus ATCC 26933 (350 ng/mg hyphae). Survival probability plots (Kaplan-Meier) of experimental groups infected with Aspergillus conidia indicate that G. mellonella is more susceptible to fungal infection by A. fumigatus ATCC 26933, implicating a critical role for gliotoxin production rather than growth rate or enzymatic activity in the virulence of A. fumigatus in this model.

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“…At least six types of hemocytes have been identified in insects such as Galleria mellonella (the greater wax moth) (7), and plasmatocytes and granulocytes are the most abundant phagocytic cell types. NADPH oxidase and NO synthase pathways have been observed in the self-defense system of Mytilus galloprovincialis (1), and production of ROS has also been detected in hemocytes; evidence of both O 2 Ϫ (32) and its dismutation product, H 2 O 2 , has been found in plasmatocytes of Drosophila melanogaster larvae (51) and G. mellonella (62).…”

mentioning

confidence: 99%

Superoxide Production in Galleria mellonella Hemocytes: Identification of Proteins Homologous to the NADPH Oxidase Complex of Human Neutrophils

et al. 2005

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The insect immune response has a number of structural and functional similarities to the innate immune response of mammals. The objective of the work presented here was to establish the mechanism by which insect hemocytes produce superoxide and to ascertain whether the proteins involved in superoxide production are similar to those involved in the NADPH oxidase-induced superoxide production in human neutrophils. Hemocytes of the greater wax moth (Galleria mellonella) were shown to be capable of phagocytosing bacterial and fungal cells. The kinetics of phagocytosis and microbial killing were similar in the insect hemocytes and human neutrophils. Superoxide production and microbial killing by both cell types were inhibited in the presence of the NADPH oxidase inhibitor diphenyleneiodonium chloride. Immunoblotting of G. mellonella hemocytes with antibodies raised against human neutrophil phox proteins revealed the presence of proteins homologous to gp91 phox , p67 phox , p47 phox , and the GTP-binding protein rac 2. A protein equivalent to p40

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EPR Detection of Reactive Oxygen Species in Hemolymph of Galleria mellonella and Dendrolimus superans sibiricus (Lepidoptera) Larvae

Cited by 60 publications

References 17 publications

Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice ofBombyx mori

Identification of a Soluble NADPH Oxidoreductase (BmNOX) with Antiviral Activites in the Gut Juice ofBombyx mori

Correlation between Gliotoxin Production and Virulence of Aspergillus fumigatus in Galleria mellonella

Superoxide Production in Galleria mellonella Hemocytes: Identification of Proteins Homologous to the NADPH Oxidase Complex of Human Neutrophils

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