EPR Oxygen Imaging Workflow with MATLAB Image Registration Toolbox

Epel, Boris; Halpern, Howard J.

doi:10.1007/s00723-021-01381-8

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…to obtain the images and image statistics using ArbuzGUI developed by Dr. Boris Epel at the University of Chicago 57 . The typical number of voxels ranged from ~300 to ~800 per well.…”

Section: Data Collectionmentioning

confidence: 99%

Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Hameed,

Viswakarma,

Babakhanova

et al. 2024

npj Imaging

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The use of oxygen by cells is an essential aspect of cell metabolism and a reliable indicator of viable and functional cells. Viable and functional cells are essential for optimizing the therapeutic dose for cell therapy, tissue engineering, drug development, and many other biological processes and products. However, currently, there is no method to assess the cell metabolic activity nondestructively in 3D space and longitudinally as cells proliferate, metabolize, differentiate, or die. Here, we report partial pressure oxygen (pO2) mapping of live cells as a reliable indicator of viable and metabolically active cells. For pO2 imaging, we utilized trityl OX071-based pulse electron paramagnetic resonance oxygen imaging (EPROI), in combination with a 25 mT EPROI instrument, JIVA-25™, that provides 3D oxygen maps in tissues with high spatial and temporal resolution. To perform oxygen imaging in an environment-controlled apparatus using a standard biological lab consumable, that is, a multi-well plate, we developed a novel multi-well-plate incubator-resonator (MWIR) system that could accommodate 3 strips from a 96-well strip-well plate and image the middle 12 wells noninvasively and simultaneously. The MWIR system was able to keep a controlled environment (temperature at 37 °C, relative humidity between 70% - 100%, and a controlled gas-flow environment) during oxygen imaging and could keep cells alive for up to 24 h of measurement, providing a rare previously unseen longitudinal perspective of 3D cell metabolic activities. The robustness of MWIR was tested using an adherent cell line (HEK-293 cells), a nonadherent cell line (Jurkat cells), a cell-biomaterial construct (Jurkat cells seeded in a hydrogel), and a negative control (dead HEK-293 cells). Using MWIR, we demonstrate that EPROI is a versatile and robust method that can be utilized to observe the cell metabolic activity nondestructively, longitudinally, and in 3D. For the first time, we demonstrated that oxygen concentration in a multi-well plate seeded with live cells is inversely proportional to the cell seeding density, even if the cells are exposed to incubator-like gas conditions (95% air and 5% CO2). Additionally, for the first time, we also demonstrate 3D, longitudinal oxygen imaging can be used to assess cells seeded in a hydrogel scaffold. These results demonstrate nondestructive, longitudinal 3D assessment of metabolic activities of cells using EPROI during 2D planar culture and during culture in a 3D scaffold system. The MWIR and EPROI approach may be useful for characterizing cell therapies, tissue engineered medical products and other advanced therapeutics.

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“…to obtain the images and image statistics using ArbuzGUI developed by Dr. Boris Epel at the University of Chicago 57 . The typical number of voxels ranged from ~300 to ~800 per well.…”

Section: Data Collectionmentioning

confidence: 99%

Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Hameed,

Viswakarma,

Babakhanova

et al. 2024

npj Imaging

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“…Using a predetermined calibration obtained for linear fit between pO 2 and R 1 (slope: 112.93 torr/Ms‐1, intercept: 0.1246) in vitro for 1 mM trityl in phosphate‐buffered saline at 37°C, R 1 maps were converted to pO 2 maps. The masks were created from the baseline SPI for each mouse using the ArbuzGUI MATLAB toolbox 68 . For statistics, the mask was transferred to the frame of each image.…”

Section: Methodsmentioning

confidence: 99%

“…The masks were created from the baseline SPI for each mouse using the ArbuzGUI MATLAB toolbox. 68 For statistics, the mask was transferred to the frame of each image. Oxygen concentration in each voxel was computed from the known, calibrated relationship between OX071 spin-lattice relaxation rate and phantoms with known oxygen concentration.…”

Section: Imaging Acquisition and Processing Parametersmentioning

confidence: 99%

Assessment of blood–brain barrier leakage and brain oxygenation in Connexin‐32 knockout mice with systemic neuroinflammation using pulse electron paramagnetic resonance imaging techniques

Epel,

Viswakarma,

Hameed

et al. 2024

Magnetic Resonance in Med

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PurposeThe determination of blood–brain barrier (BBB) integrity and partial pressure of oxygen (pO2) in the brain is of substantial interest in several neurological applications. This study aimed to assess the feasibility of using trityl OX071‐based pulse electron paramagnetic resonance imaging (pEPRI) to provide a quantitative estimate of BBB integrity and pO2 maps in mouse brains as a function of neuroinflammatory disease progression.MethodsFive Connexin‐32 (Cx32)–knockout (KO) mice were injected with lipopolysaccharide to induce neuroinflammation for imaging. Three wild‐type mice were also used to optimize the imaging procedure and as control animals. An additional seven Cx32‐KO mice were used to establish the BBB leakage of trityl using the colorimetric assay. All pEPRI experiments were performed using a preclinical instrument, JIVA‐25 (25 mT/720 MHz), at times t = 0, 4, and 6 h following lipopolysaccharide injection. Two pEPRI imaging techniques were used: (a) single‐point imaging for obtaining spatial maps to outline the brain and calculate BBB leakage using the signal amplitude, and (b) inversion‐recovery electron spin echo for obtaining pO2 maps.ResultsA statistically significant change in BBB leakage was found using pEPRI with the progression of inflammation in Cx32 KO animals. However, the change in pO2 values with the progression of inflammation for these animals was not statistically significant.ConclusionsFor the first time, we show the ability of pEPRI to provide pO2 maps in mouse brains noninvasively, along with a quantitative assessment of BBB leakage. We expect this study to open new queries from the field to explore the pathology of many neurological diseases and provide a path to new treatments.

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“…Besides this process, letters and symbols could directly use ASCII code; however, medical images needed to be interpreted before they could be transmitted by the network. Image Acquisition [30] was employed to divide images into three color channels. The overlapping of the codes between the figure and letter may have allowed additional decoding errors.…”

Section: Transmission Protocolmentioning

confidence: 99%

Distributed Diagnoses Based on Constructing a Private Chain via a Public Network

Li,

Zhan

2023

Entropy

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Secure online consultations can provide convenient medical services to patients who require experts from different regions. Moreover, this process can save time, which is critical in emergency cases, and cut medical costs. However, medical services need a high level of privacy protection that advances the difficulty of a construction method. It is a good idea to construct a virtual private chain through public networks by means of cryptology and identity verification. For this purpose, novel protocols are proposed to finish the package layout, secure transmission, and authorization. By mining the special characteristics of this application, two different kinds of encryption channels were designed to support the proposed protocol to ensure the secure transmission of data. And Hash values and multiple checking were employed in the transmission package to find the incompleteness of data related to network errors or attacks. Besides the secure communication of medical information, the Extended Chinese Remainder Theorem was utilized to finish the approval during a change in committee in emergency situations. Finally, example case was used to verify the effectiveness of the total methods.

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EPR Oxygen Imaging Workflow with MATLAB Image Registration Toolbox

Cited by 8 publications

References 27 publications

Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Nondestructive, longitudinal, 3D oxygen imaging of cells in a multi-well plate using pulse electron paramagnetic resonance imaging

Assessment of blood–brain barrier leakage and brain oxygenation in Connexin‐32 knockout mice with systemic neuroinflammation using pulse electron paramagnetic resonance imaging techniques

Distributed Diagnoses Based on Constructing a Private Chain via a Public Network

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