EPR spin labeling measurements of thylakoid membrane fluidity during barley leaf senescence

Jajić, Ivan; Wiśniewska-Becker, Anna; Sarna, Tadeusz; Jemioła-Rzemińska, Małgorzata; Strzałka, Kazimierz

doi:10.1016/j.jplph.2014.03.017

Cited by 13 publications

(15 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At later stage of senescence, when thylakoid membranes become more fluid and more leaky, the effect of uncoupling although still present, is not so pronounced. The results indicate that H 2 O 2 formation in chloroplasts is strongly connected with the activity of thephotosynthetic electron transport and further confirm conclusions from our previous study where increase in membrane fluidity during senescence of barley was observed (Jajić et al, 2014). The estimation of O • 2 − by the EPR-spin trapping method showed that the production of O • 2 − is detectable as early as 18 DAS, reaching the highest values at 29 DAS.…”

Section: Discussionsupporting

confidence: 91%

“…This study is a continuation of our previous work, in which we investigated the physical properties of barley thylakoid membranes during the course of senescence, and how changes in the levels of different carotenoids and proteins impact these changes (Jajić et al, 2014). In this article, we investigate the production of three main ROS; O • 2 − , H 2 O 2 , and 1 O 2 during the development and senescence of barley leaves by using highly advanced methods for the specific detection of ROS.…”

Section: Introductionmentioning

confidence: 78%

See 1 more Smart Citation

Changes in production of reactive oxygen species in illuminated thylakoids isolated during development and senescence of barley

Jajić

Sarna

Szewczyk

et al. 2015

Journal of Plant Physiology

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 78%

Changes in production of reactive oxygen species in illuminated thylakoids isolated during development and senescence of barley

Jajić

Sarna

Szewczyk

et al. 2015

Journal of Plant Physiology

Self Cite

View full text Add to dashboard Cite

“…The FT-IR analysis of the thylakoid membranes showed an increased lipid:protein ratio in thylakoid membranes of T. pseudonana cells cultured at higher temperature (Fig. 8), what also affected the membrane fluidity [39]. Additionally, we also observed an increase of the protein:chlorophyll as well as the protein:OD600 ratio in intact T. pseudonana cells cultured at lower temperatures (Fig.…”

Section: Resultsmentioning

confidence: 60%

Acclimatization of Thalassiosira pseudonana Photosynthetic Membranes to Environmental Temperature Changes

Bojko¹,

Olchawa-Pajor²,

Chyc³

et al. 2017

Proceedings of the 2nd World Congress on New Technologies

View full text Add to dashboard Cite

-The greenhouse effect results in warming the planet's surface and a higher ocean heat content. It causes changes of the water circulation frequency and turbulent mixing, delivering warm water, phytoplankton nutrients and CO2 from deeper layers of water to the water surface. All of these changes can have an impact on Earth's ecosystems. In the present study the effect of changing environmental temperature on a model diatom species, Thalassiosira pseudonana was tested under laboratory conditions. The purpose of the work was to analyze the temperature effect on fluidity and chemical composition of the thylakoid membranes. The photosynthetic activity and photosynthetic pigment contents of diatoms adapted to different temperatures were also determined. Our result show decreases of the growth rate and chlorophylls concentration in diatom cells cultured at lower temperature. It was also detected that increases of the polyunsaturated and decreases of saturated fatty acids, as well as changes in lipids:proteins ratio, resulted in stabilization of the thylakoid membranes fluidity and photosynthetic efficiency (Fv/Fm) in a physiological range of the temperature. The result show that the regulation of the concentration and interaction of fatty acids, proteins and pigments is the most important factor in the adaptation strategies of diatoms to the environmental temperature changes. Additionally, the regulation of membrane fluidity was demonstrated as important mechanism of the diatom adaptation to greenhouse effects. We also postulate that the adaptive mechanisms to temperature changes are differently expressed in the polar or hydrophobic regions of photosynthetic membranes.

show abstract

“…The influence of organo-metallic complexes on the surface layer of membranes and theoretical considerations have been described [27,28]. Experiments with trans-membrane voltage confirmed significant differences between the effects of HA-A and HA-B on the membranes.…”

Section: Discussionmentioning

confidence: 94%

“…Presumably, a membrane -based liquid crystal can not 'see' HAs molecules as clearly as when they are in the gel phase. Computer simulations have shown [26][27][28] that in the liquid crystal phase, the membrane may have numerous gaps in the hydrophilic part. This is related to the mobility of molecules, and therefore the ability to dissolve the admixtures in a given phase.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Natural and Model Forms of Humic Acids on the Dynamic Parameters of Model Membranes

Pisarek

Pytel

Filipiak³

et al. 2016

Ecological Chemistry and Engineering S

View full text Add to dashboard Cite

Abstract:In this paper the influence of humic acid concentrations extracted from Histosols (HA-A) and their model forms (HA-B) separated from humic substances commercially produced by Carl Roth GmbH + Co.KG on the dynamic properties of liposome membranes was determined. Differences in the quality of the humic acids (HA-A and HA-B) were determined by the 1 HNMR and FTIR methods. Liposomes from the sonication of egg yolk lecithin (EYL) in an aqueous solution and synthetic Dipalmitoylphosphatidylcholine (DPPC) were used. Fluidity of liposome membranes was determined by the EPR technique with spin probes (TEMPO, 16DOIXYL). The electrical parameters of membranes were found using a Keithley 6517 electrometer. Our study showed significant differences in the influence of HA-A and HA-B on the membranes. In the bilayer membranes of the liposomes of HA-A admixture there was slightly more stiffening of the interior of the membrane in comparison to HA-B. A similar effect was observed in the surface layer of the liposome membranes. This difference is particularly evident for DPPC liposomes, however, the EYL liposomes admixture with HA-B slightly increased the fluidity of the surface layer. Electrical study confirmed this effect. The study shows that natural and model forms of humic acids differ in their effects on the activity of tested membrane models. The strong differences in the interaction of HA-A and HA-B on parameter F in DPPC liposomes can be result from the transport of humic acids connected to the metal ions inside the membranes (xenobiotics present in the environment).

show abstract

EPR spin labeling measurements of thylakoid membrane fluidity during barley leaf senescence

Cited by 13 publications

References 42 publications

Changes in production of reactive oxygen species in illuminated thylakoids isolated during development and senescence of barley

Changes in production of reactive oxygen species in illuminated thylakoids isolated during development and senescence of barley

Acclimatization of Thalassiosira pseudonana Photosynthetic Membranes to Environmental Temperature Changes

The Influence of Natural and Model Forms of Humic Acids on the Dynamic Parameters of Model Membranes

Contact Info

Product

Resources

About