Epsin 3 potentiates the NF‑κB signaling pathway to regulate apoptosis in breast cancer

Wu, Qianxue; Li, Qing; Zhu, Wenming; Zhang, Xiang; Li, Hongyuan

doi:10.3892/mmr.2021.12531

Cited by 3 publications

(9 citation statements)

References 30 publications

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“…The expression patterns of SYF2 and CPN2 in other breast cancer studies and in studies of other tumor types were both opposite to the findings of this study [ 22 , 23 , 24 , 25 ]. In addition, EPN3 was upregulated in other breast cancer-related studies, similar to this study, but was downregulated in gastric cancer and upregulated in glioblastoma, with the opposite results [ 26 , 27 , 28 ]. SPINT2 showed various expression patterns in tumors.…”

Section: Discussionsupporting

confidence: 79%

Discovering Breast Cancer Biomarkers Candidates through mRNA Expression Analysis Based on The Cancer Genome Atlas Database

Kim

Lee

2022

JPM

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Background: Research on the discovery of tumor biomarkers based on big data analysis is actively being conducted. This study aimed to secure foundational data for identifying new biomarkers of breast cancer via breast cancer datasets in The Cancer Genome Atlas (TCGA). Methods: The mRNA profiles of 526 breast cancer and 60 adjacent non-cancerous breast tissues collected from TCGA datasets were analyzed via MultiExperiment Viewer and GraphPad Prism. Diagnostic performance was analyzed by identifying the pathological grades of the selected differentially expressed (DE) mRNAs and the expression patterns of molecular subtypes. Results: Via DE mRNA profile analysis, we selected 14 mRNAs with downregulated expression (HADH, CPN2, ADAM33, TDRD10, SNF1LK2, HBA2, KCNIP2, EPB42, PYGM, CEP68, ING3, EMCN, SYF2, and DTWD1) and six mRNAs with upregulated expression (ZNF8, TOMM40, EVPL, EPN3, AP1M2, and SPINT2) in breast cancer tissues compared to that in non-cancerous tissues (p < 0.001). Conclusions: In total, 20 DE mRNAs had an area under cover of 0.9 or higher, demonstrating excellent diagnostic performance in breast cancer. Therefore, the results of this study will provide foundational data for planning preliminary studies to identify new tumor biomarkers.

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Section: Discussionsupporting

confidence: 79%

Discovering Breast Cancer Biomarkers Candidates through mRNA Expression Analysis Based on The Cancer Genome Atlas Database

Kim

Lee

2022

JPM

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“…48 Previous study has suggested that EPN3 silencing could suppress the progression of breast cancer via inhibiting the NF-κB pathway. 10 This was the first time that EPN3 silencing was demonstrated to inhibit the progression of NSCLC by inactivating the JAK1/2-STAT3 pathway. This discovery provided favorable molecular evidence for EPN3 to be a target for NSCLC treatment.…”

Section: Loss Of Epn3 Might Suppress the In Vivo Growth Of Nsclc Cell...mentioning

confidence: 98%

“…In some human tumors, such as breast cancer and glioma, EPN3 acts as a cancer-promoting gene. 10,11 However, it exerts cancer suppression role in gastric cancer. 13 This study demonstrated that EPN3 was an oncogene in NSCLC.…”

Section: Loss Of Epn3 Might Suppress the In Vivo Growth Of Nsclc Cell...mentioning

confidence: 99%

“…9 Epsin 3 (EPN3) is a member of the Epsin family. 10 Recently, EPN3 was discovered to induce migration, invasion and epithelial-mesenchymal transition (EMT) in glioma cells. 11 EPN3 was revealed to be over-expressed in clinical breast cancer tissues.…”

mentioning

confidence: 99%

“…Loss of EPN3 repressed the proliferation and induced the apoptosis in breast cancer cells. 10 Moreover, the highly expressed EPN3 was related to the increased risk of breast cancer late metastasis. 12 However, the function of EPN3 varies depending on the type of cancer.…”

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confidence: 99%

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EPN3 plays oncogenic role in non‐small cell lung cancer by activating the JAK1/2‐STAT3 pathway

Meng

Zhang

Zhu

et al. 2023

Environmental Toxicology

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The effect of Epsin 3 (EPN3) on non‐small cell lung cancer (NSCLC) has not yet been clearly elucidated. This study identified the exact function of EPN3 on NSCLC progression. EPN3 expression in NSCLC patients were analyzed based on the Cancer Genome Atlas database. Kaplan–Meier analysis was implemented to research the effect of EPN3 on patients' survival. EPN3 expression in clinical tissues of 62 NSCLC cases was monitored by real‐time quantitative reverse transcription polymerase chain reaction, immunohistochemistry and Western blot. A549 and H1299 cells were transfected with EPN3 shRNA and treated by RO8191 (20 μM). Proliferation was researched by cell counting kit‐8 and 5‐ethnyl‐2 deoxyuridine assays. Apoptosis was monitored by flow cytometry. Migration and invasion was assessed by Transwell experiment. EPN3 effect on A549 cell in vivo growth was researched using nude mice. RO8191 (200 μg) was intratumoral injected into mice. Immunohistochemistry and Western blot was implemented to monitor protein expression in cells and xenograft tumor tissues. EPN3 was abnormally up‐regulated in NSCLC patients and cells, indicating a lower overall survival. Loss of EPN3 weakened proliferation, migration and invasion, induced apoptosis, and repressed epithelial‐mesenchymal transition in NSCLC cells. Loss of EPN3 inactivated the JAK1/2‐STAT3 pathway in NSCLC cells. RO8191 treatment reversed the inhibition of EPN3 knockdown on the malignant phenotype of NSCLC cells. RO8191 intratumoral injection reversed the suppression of EPN3 silencing on NSCLC cell in vivo growth. EPN3 acted as an oncogene in NSCLC via activating the JAK1/2‐STAT3 pathway. EPN3 may be a promising target for NSCLC treatment.

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Tumor-associated inflammation: The role and research progress in tumor therapy

Xu,

Zhang

et al. 2024

Journal of Drug Delivery Science and Technology

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Epsin 3 potentiates the NF‑κB signaling pathway to regulate apoptosis in breast cancer

Cited by 3 publications

References 30 publications

Discovering Breast Cancer Biomarkers Candidates through mRNA Expression Analysis Based on The Cancer Genome Atlas Database

Discovering Breast Cancer Biomarkers Candidates through mRNA Expression Analysis Based on The Cancer Genome Atlas Database

EPN3 plays oncogenic role in non‐small cell lung cancer by activating the JAK1/2‐STAT3 pathway

Tumor-associated inflammation: The role and research progress in tumor therapy

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