Epstein-Barr Virus DNA Sequences in Precursor Monocyte-Macrophage Cell Lines Established from the Bone Marrow of Children with Maturation Defects of Haematopoiesis

Abstract

SUMMARYEpstein-Barr virus (EBV) DNA sequences were detected in four established monoblast or early monocytic cell lines (CM-S, ROV-S, CV-S and AD-S) obtained from bone marrow of children suffering from maturation defects of haematopoiesis. EBV is present in these cells in a latent state. The viral DNA in these cell lines was analysed by Southern blot hybridization, using a set of cloned EBV DNA fragments from the EBV strain B95-8 as probes. A common spectrum of highly related but distinguishable EBV DNA restriction enzyme sequences was found, suggesting some genomic diversity. Propagation of the cells in long-term culture revealed a gradual decrease of EBV copies per cell in all lines with some minor changes in the restriction pattern of the EBV DNA. These findings demonstrate that human precursor monocyte cells may be susceptible to infection by EBV.

Immature and Transitional B Cells Are Latency Reservoirs for a Gammaherpesvirus

“…Other, more tenuous links to human disease have been reported for EBV following detection of viral genome in the bone marrow. For example, latent EBV genome was detected in precursor monocyte-macrophage cell lines that were established from the bone marrow of children with maturation defects of hematopoiesis (30), and EBV sequences were identified in bone marrow mononuclear cells in 51% of samples from various bone marrow hematopoietic malignancies, including multiple myeloma, acute myelocytic leukemia, chronic myelocytic leukemia, and acute lymphocytic leukemia (39).…”

Fatal Aplastic Anemia Caused by Epstein-Barr Virus Infection after Autologous Bone Marrow Transplantation for Non-Hodgkin Malignant Lymphoma.

“…EB virus nuclear antigen (EBNA) is detected by immunofluorescence and EB virus DNAis detected by in situ hybridization in B cell-depleted bone marrow cells (3 , 4). EBvirus DNAis also observed in the monocyte-macrophage precursors (12). The typical strains of EB virus are not inhibitory of normal hematopoiesis in vitro, but EB virus-exposed cells may be more susceptible to T cell-mediated cytotoxicity (3).…”

“…While the B cells are the recognized target ofEB virus infection, other cells mayalso harbor this virus (e.g., nasopharyngeal epithelial cells). Somehematopoietic cells may also be infected by EB virus (3,4,12). EB virus nuclear antigen (EBNA) is detected by immunofluorescence and EB virus DNAis detected by in situ hybridization in B cell-depleted bone marrow cells (3 , 4).…”

Expression of Epstein--Barr virus latent membrane protein influences self-renewal and differentiation in a multipotential murine haemopoietic 'stem cell' line

“…Recently it has been proposed that expression of EBV proteins in myeloid cells may be associated with defects in their maturation (Revoltella et al, 1989), and this led us to examine the effects of LMP expression in a murine myeloid progenitor cell line.…”

“…Recent evidence suggests that EBV may also have a role to play in some myelopoietic disorders, in that the EBV genome and some EBV-encoded proteins have been detected in cell lines of the monocyte/macrophage lineage isolated from children with maturation defects in myelopoiesis (Revoltella et al, 1989). Although no causal relationship has been established between EBV and these various defects, it is tempting to speculate that the same genes which are primary candidates for mediating the effects of EBV on B lymphocytes and 0001-1155 © 1993 SGM epithelial cells might also contribute to the pathology of these maturation defects.…”