Epstein-Barr Virus in an American Patient With Burkitt's Lymphoma: Detection of Viral Genome in Tumor Tissue and Establishment of a Tumor-Derived Cell Line (NAB)2

Gravell, Maneth; Levine, P. H.; McIntyre, Rosemary; Land, Vita J.; Pagano, J. S.

doi:10.1093/jnci/56.4.701

Cited by 37 publications

(7 citation statements)

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“…Actively growing, subcon¯uent cultures were used. NAB-2, a Burkitt lymphoma cell line (Gravell et al, 1976) was cultured as previously described (Popescu et al, 1990c).…”

Section: Cell Culturementioning

confidence: 99%

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

1997

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Human Papillomavirus (HPV) type 16 is the most frequently detected HPV in cervical cancer. Although epidemiologic and experimental evidence indicates a prominent role for HPV infection in the development of this disease, other factors are also involved. Altered expression of the ets family transcription factors erg and ets-2 was found associated with the development of cervical carcinoma. Overexpression also occurred in a HPV-16-immortalized cervical cell line, CX16-2, which has HPV integrated at a translocation breakpoint t(19;21) involving 21q22.2-22.3, where these genes have been mapped. Six of 10 cervical carcinoma cell lines overexpressed ets-2 RNA suggesting an association of overexpression with cervical cell neoplasia. A clonally related pair of cervical carcinoma cell lines, C-4I and C-4II, showed di erential expression of erg and ets-2. C-4I overexpressed ets-2 RNA compared to normal cervical cells and C-4II. C-4II expressed a 5.3 kb erg transcript not seen in C-4I, ectocervical cells or other cervical carcinoma cell lines examined. Pulsed ®eld gel electrophoresis was used to analyse changes in DNA fragments related to structural changes and to construct a physical map encompassing erg and ets-2. Alterations in erg and ets-2 RNA expression in each of three di erent cell lines examined were associated with translocations. Association between altered expression of erg and ets-2 and altered regional structural suggests that these genes are important targets in cervical carcinogenesis.

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“…Actively growing, subcon¯uent cultures were used. NAB-2, a Burkitt lymphoma cell line (Gravell et al, 1976) was cultured as previously described (Popescu et al, 1990c).…”

Section: Cell Culturementioning

confidence: 99%

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

1997

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“…11 The NAB-2 line, a clonal derivative of the original culture, exhibited t(8;22) (q22;q11-12) involving the c-myc gene and lambda light chain of immunoglobulin genes. 12 NAB-2 was reported to contain one copy of EBV DNA, which is integrated into the host genome.…”

mentioning

confidence: 99%

Epstein–Barr virus is integrated between REL and BCL-11A in American Burkitt lymphoma cell line (NAB-2)

Luo

Takakuwa

Ham

et al. 2004

Laboratory Investigation

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Epstein-Barr virus (EBV) initially isolated from the cultured Burkitt lymphoma (BL) cells, is one of the wellknown oncogenic virus. The NAB-2 line, which was established from a North American Burkitt's tumor, was indicated to contain one copy of EBV DNA as the integrated form into chromosome 2p13 of the host genome. To demonstrate the integration site of EBV directly, and to clarify the relation between the integration sites and the oncogenes, fragments containing the nucleotide sequence of NAB-2 integration sites were cloned. EBV was integrated via the terminal repeats (TR), and integration sites located in the clone RP11-440P5 on chromosome 2, between two oncogenes, REL and BCL11A, which is apart from approximately 350 kbp from each other. Expression level of REL in NAB-2 was increased. The flanking region of chromosome 2 at the bilateral junction sites showed no homology to the junction sites of EBV. The integration site 2p13 overlaps with common fragile site, FRA2E. NAB-2 cells expressed almost all latent genes but LMP-2A that flanks the TR, indicating the type III of latent infection of EBV. Integration event in NAB-2 might alter the regulation of the oncogenes and provide advantage for continuous cell proliferation.

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“…As compared to African patients, EBV-associated American or European cases of BL cover an older age range, including young adults. These patients show a spectrum and titers of EBV-specific antibodies characteristic of their African counterparts IAndersson-Anvret et al, 1976;Gravell et al, 1976). Even a cluster of four young adult cases of EBV-associated BL living within 30 miles of each other has been observed in Pennsylvania within 1 year .…”

Section: B Sporadic Bl In Caucasian Patientsmentioning

confidence: 99%

The Herpesviruses

1982

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Epstein-Barr Virus in an American Patient With Burkitt's Lymphoma: Detection of Viral Genome in Tumor Tissue and Establishment of a Tumor-Derived Cell Line (NAB)2

Cited by 37 publications

References 0 publications

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

Epstein–Barr virus is integrated between REL and BCL-11A in American Burkitt lymphoma cell line (NAB-2)

The Herpesviruses

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