2016
DOI: 10.1155/2016/3214105
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Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway

Abstract: Epstein-Barr virus-induced gene 3 (EBI3) is a member of the interleukin-12 (IL-12) family structural subunit and can form a heterodimer with IL-27p28 and IL-12p35 subunit to build IL-27 and IL-35, respectively. However, IL-27 stimulates whereas IL-35 inhibits antitumor T cell responses. To date, little is known about the role of EBI3 in tumor microenvironment. In this study, firstly we assessed EBI3, IL-27p28, IL-12p35, gp130, and p-STAT3 expression with clinicopathological parameters of colorectal cancer (CRC… Show more

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Cited by 29 publications
(30 citation statements)
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“…As our previously reported [49,50], the tumor tissue were fixed in formalin, embedded in paraffin, sectioned and then heat-immobilized, quenched the endogenous peroxidase activity and retrieved antigenic epitopes. For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).…”
Section: Immunohistochemistry (Ihc)mentioning
confidence: 99%
See 1 more Smart Citation
“…As our previously reported [49,50], the tumor tissue were fixed in formalin, embedded in paraffin, sectioned and then heat-immobilized, quenched the endogenous peroxidase activity and retrieved antigenic epitopes. For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).…”
Section: Immunohistochemistry (Ihc)mentioning
confidence: 99%
“…Cell apoptosis was evaluated by flow cytometric analysis following staining with the APC Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, CA, USA) according to manufacturer's protocol as described before [50,51]. The BD FACS Calibur II (San Jose, CA, USA) platform was used to acquire data.…”
Section: Cell Apoptosis Assaymentioning
confidence: 99%
“…Induced in B lymphocytes by the Epstein-Barr virus (EBV) infection, EBI3 has been found in EBV-associated tumors, nasopharyngeal carcinoma, and Hodgkin lymphoma to inhibit an effective antitumor immune response, independent of its association to IL-30 [58,59]. EBI3 has revealed growth-promoting activity in lung cancer [60] and in colorectal cancer, by stimulating cell proliferation, via the gp130/STAT3 axis, and by restraining tumor infiltrating granzyme B + CTLs and IFNγ + CTLs [61], thus, allowing the cancer to escape immune surveillance.…”
Section: Ebi3 Il-35 and Il-39 Involvement In Cancer-myeloid Cell Crmentioning
confidence: 99%
“…Then cells were stained with PE‐conjugated anti‐mouse IgG (MOPC‐21, BioLegend, USA) for 30 minutes after wash twice with PBS. Finally, CT83‐positive cells were analyzed by flow cytometry (BD FACS Calibur II, San Jose, CA, USA) as we previously described . Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., USA).…”
Section: Methodsmentioning
confidence: 99%