Epstein‐Barr virus involvement is mainly restricted to lymphoepithelial type of gastric carcinoma among various epithelial neoplasms

Kijima, Yuko; Hokita, Shuichi; Takao, Sonshin; Baba, Masamichi; Natsugoe, Shoji; Yoshinaka, Heiji; Aridome, Kuniaki; Otsuji, T; Itoh, Tetsuhiko; Tokunaga, Masayoshi; Eizuru, Yoshito; Aikou, Takashi

doi:10.1002/jmv.1079

Cited by 76 publications

(66 citation statements)

References 26 publications

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“…In our study, 1 case of adenoma complicated with dysplasia showed positive signals for EBER. This finding was different from the observation of Kijima et al, Yuen et al and Cho et al [21,22,24] . Moreover, detection of EBV in 1 case of dysplastic adenoma suggested that EBV infection occurred in the dysplastic phase before the occurrence of colorectal carcinoma, further indicating that EBV may play a role in tumor progression.…”

Section: Discussioncontrasting

confidence: 90%

“…Yuen et al [22] investigated for the presence of EBV in 74 cases of gastric adenocarcinoma and 36 cases of colorectal adenocarcinoma from Chinese patients by in situ hybridization (ISH) using an antisense EBER probe, but none of the colorectal carcinomas showed a positive signal. Kijima et al [21] demonstrated the association of Epstein-Barr virus (EBV) with primary epithelial neoplasm in the south part of Kyushu, Japan, they found that there were no positive signals in 102 cases of colorectal cancer using EBER in situ hybridization. Cho et al [24] reported the same result that EBV was not associated with colorectal tumors.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of Epstein-barr virus in Chinese colorectal tumors

Liu¹,

Ding²,

Li³

et al. 2003

WJG

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AIM:To elucidate the association of Epstein-Barr virus (EBV) with colorectal tumors and to demonstrate whether infection of EBV existed in different stages of colorectal tumors involves in the carcinogenesis. METHODS:One hundred and thirty paraffin-embedded tissues of colorectal tumors were classified into 5 groups: 26 adenomas, 23 adenomas complicated with dysplasia, 22 adenomas complicated with carcinomatous, 36 colon carcinoma and 23 HNPCC, were examined by PCR, IHC and ISH, respectively. RESULTS: EBV DNA was detected by PCR in 26 cases out of the 130 specimens, including 5 cases of adenomas, 5 adenomas complicated with dysplasia, 5 adenomas complicated with carcinomatous, 7 colorectal carcinoma and 4 HNPCC. IHC detection showed the expression of LMP1 in 7 cases, including 1 adenoma, 1 adenoma with dysplasia, 1 HNPPC, 2 adenomas complicated with carcinomatous, and 2 colorectal carcinomas. The expression of EBER1 detected by ISH was positive in 6 cases, including 1 adenoma with dysplasia, 2 adenomas complicated with carcinomatous and 3 colorectal carcinomas. There were no significant differences among the results of PCR, IHC and ISH in the 5 groups. In all cases of HNPCC, none of the tumor cells showed positive signals of EBER1, but some EBV-positive tumor infiltrating lymphocytes were found in 2 of 23 cases. CONCLUSION:Our results showed that infection of EBV exists in human colorectal tumors, which indicates that EBV may be involved in the carcinogenesis of colorectal tumors but does not play an important role. The mechanisms need to be clarified further.Liu HX, Ding YQ, Li X, Yao KT. Investigation of Epstein-barr virus in Chinese colorectal tumors.

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Section: Discussioncontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Investigation of Epstein-barr virus in Chinese colorectal tumors

Liu¹,

Ding²,

Li³

et al. 2003

WJG

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“…Therefore, significant differences are observed in terms of cell differentiation and viral gene expression in epithelial malignancies associated with EBV [8]. In addition, it appears that the relationship between EBV and certain epithelial neoplasia depends on the patients' regional or ethnic backgrounds [7,25]. Perhaps one reason for the lower prevalence of EBV in patients with CRC compared to patients with gastric carcinoma is the preferential residence of EBV in the upper gastrointestinal tract and lympho-hematopoietic tissues compared to the colorectal region [26].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the lack of a significant association between EBV detection and the incidence of cancer, relevant research has demonstrated the contribution of this virus to the progression of adenocarcinoma and carcinogenesis [25,31,33,35,36].…”

Section: Discussionmentioning

confidence: 99%

Original paper Epstein-Barr virus DNA in colorectal carcinoma in Iranian patients

Tafvizi¹,

Fard²,

Assareh³

2015

pjp

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Colorectal cancer is an often fatal cancer with a rapidly increasing incidence. Current mortality is estimated to be approximately 600,000 per year, and both environmental and genetic factors are involved in its etiology. Viral and bacterial factors have a proven role in the incidence of approximately 20% of cancers. In the present study, the Epstein-Barr virus (EBV) was detected in 50 colorectal adenocarcinomas, 12 colon adenomas, and 38 control tissue samples using polymerase chain reaction (PCR). Epstein-Barr virus DNA was identified in 19 of the adenocarcinoma tissues, 1 adenoma tissue and 24 control specimens. In total, 15.8% (3/18) of the colorectal samples in the well-differentiated grade, 79% (15/30) in the moderately differentiated, and 5.2% (1/2) in the poorly differentiated grade tested positive for viral infection. Epstein-Barr virus was more prevalent in the moderately differentiated grade. Statistical analysis did not suggest a significant association between EBV and the incidence of colorectal cancer. However, it appears that the virus stimulates progression of the malignancy.

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“…The infection rate of EBV was significantly higher in HCC patients than in control patients. [20][21][22][23] , esophageal cancer [24,25] , gastric cancer and colorectal cancer. It has been reported that the correlation between EBV and tumor incidence is stronger in Japan and Taiwan than in the western countries.…”

Section: Discussionmentioning

confidence: 99%

Epstein-Barr virus in hepatocellular carcinogenesis

Bao-an²,

Zeng³

et al. 2004

WJG

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AIM:In recent years, studies have suggested that EpsteinBarr virus (EBV) is associated with HCC. The present study was to determine the prevalence of EBV in HCC patients, and whether EBV acted synergistically with hepatitis viruses in HCC carcinogenesis. METHODS:Liver tissue 115 HCC patients and 26 noncarcinoma patients were studied. Polymerase chain reaction (PCR) was performed to detect EBV BamHI W DNA, EBV LMP1 DNA, HBV X DNA, and HBV S DNA. Reverse transcription PCR (RT-PCR) was performed to detect HCV RNA and HDV RNA. Immunohistochemistry was performed to detect LMP1, HBsAg, HBcAg and HCV. The positive ratios were compared between HCC group and control group by χ 2 test. RESULTS:Totally, 78 HCC samples whose β-globulin DNA was positively detected by amplified PCR were selected. PCR was performed in all cases for EBV DNA and HBV DNA.RT-PCR was performed in 18 cases for HCV RNA and HDV RNA. EBV BamHI W and EBV LMP1 were positive in 18 and 6 cases, respectively. HBV X gene and HBV S gene were positive in 42 and 27 cases respectively. HCV was positive in one of the 18 cases, and none was positive for HDV. The positive rates were 28.2% (22 of 78) for EBV DNA (BamHI W and/or LMP1) and 56.4% (44 of 78) for HBV DNA (X gene and/or S gene) respectively. In addition, 12 cases were positive for both EBV DNA and HBV DNA. Among the 26 cases in the control group, 2 cases were positive for EBV BamHI W, 4 positive for HBV X gene and 3 positive for HBV S gene. The positive rates were 8.0% (2 of 26) and 23.1% (6 of 26), respectively, for EBV DNA and HBV DNA. The result of DNA sequencing of BamHI W was 100% homologous with the corresponding sequence of B95-8. There was significant difference in EBV infection rate between HCC patients and controls (χ 2 = 4.622, P<0.05). The difference in HBV infection rate was also significant (χ 2 = 8.681, P<0.05). However, there was no obvious correlation between HBV and EBV in HCC patients (χ 2 = 0.835, P>0.05). LMP1, HBV (HBsAg, HBcAg) and HCV were detected positively in 25, 45 and 6 of 78 cases of HCC tissues respectively. In the 26 control cases, the corresponding positive cases were 2, 4 and 0. The difference in EBV infection rate between HCC patients and control cases was statistically significant (χ 2 = 6.02, P<0.05). The difference in HBV infection rate was also statistically significant (χ 2 = 10.03, P<0.05). In the 25 cases with positive LMP1 expression, 6 were in the nuclei of tumor cells, 9 in the cytoplasm of tumor cells and 10 in mesenchymal lymphocyte cytoplasm. CONCLUSION:The existence of EBV infection in HCC tissues suggests that EBV may be involved in the hepatocellular carcinogenesis in China. HBV infection may be a major cause of HCC. There is no correlation between EBV and HBV in the development of HCC. The prevalence of HCV infection is low in our area, and HDV appears not to play a direct role in hepatocellular carcinogenesis.

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Epstein‐Barr virus involvement is mainly restricted to lymphoepithelial type of gastric carcinoma among various epithelial neoplasms

Cited by 76 publications

References 26 publications

Investigation of Epstein-barr virus in Chinese colorectal tumors

Investigation of Epstein-barr virus in Chinese colorectal tumors

Original paper Epstein-Barr virus DNA in colorectal carcinoma in Iranian patients

Epstein-Barr virus in hepatocellular carcinogenesis

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