Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová, Martina; Stádník, Luděk; Biniová, Zuzana; Ducháček, Jaromír; Stupka, Roman

doi:10.17221/23/2016-cjas

Cited by 13 publications

(16 citation statements)

References 34 publications

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“…The factors of bull's individuality or thawing method were detected as important in presented study as well as in many previous (Beran et al, 2013;Paldusová et al, 2016;Doležalová et al, 2016). Both these criteria should be therefore tested and proven in specified conditions to optimize methods of IDs processing.…”

Section: Discussionmentioning

confidence: 62%

“…Our results monitored immediately after thawing are ambiguous in this connection, when both too slow thawing method as well as rapid thawing method reached better spermatozoa motility than control method of thawing. The individuality of bull and also the semen processing influenced the results of spermatozoa motility after thawing (Robbins et al, 1976;Moore et al, 2006;Beran et al, 2011;Doležalová et al, 2016). Therefore, these factors should serve as possible explanation of various results presented in this study with those of opposite.…”

Section: Discussionmentioning

confidence: 74%

“…Therefore, the quality of produced insemination doses (ID) is directly related to fertility (Pugliesi et al, 2014). The spermatozoa survivability after cryopreservation is influenced by many factors such as type of semen extender (Moore et al, 2006), packing method, the length of equilibration, freezing rate (Rodriguez et al, 1975;Robbins et al, 1976;Doležalová et al, 2016) and finally thawing rate and handling of IDs just before using (Moore et al, 2006). Spermatozoa having reached elevated thawed temperatures may be damaged by "cold-shock" in cold or overheating in hot ambient climatic conditions (Kaproth et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Thawing Methods on Bull's Semen Characteristics

Doležalová

Ptáček

Stádník

et al. 2017

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of the study was to evaluate the influence of different thawing methods on bull's semen characteristics. The semen was collected and processed from 8 bulls (Holstein, n = 4; Czech Fleckvieh, n = 4) kept in private Sire insemination Centre. Four different thawing methods were tested: control thawing methods (temperature = 38.5 °C, time = 30 seconds), slow thawing method (temperature = 30 °C, time = 50 seconds), moderate thawing method (temperature = 50 °C, time = 15 seconds), rapid thawing rate (temperature = 70 °C, time = 3 seconds). The percentage rate of total and progressive motile spermatozoa above head as well as movement characteristics of straight-line velocity (VSL, µm/s) and linearity (LIN, %) were recorded using CASA system immediately after thawing and after 2 hrs. of heat incubation (±38 °C). Subsequently the differences between semen characteristics measured immediately after thawing and after 2 hours of incubation were calculated. The data were analyzed with SAS software. All the evaluated semen traits reached significantly lowest values in total motility (−8 % to −10.5 %, P < 0.05), progressive motility (−5.6 % to −10 %, P < 0.05) VSL (−3.9 µm/s to −9 µm/s, P < 0.05) and LIN (−1.5 % to −4.8 %, P < 0.05) in control thawing method immediately after the thawing compared to others. However, these differences were negligible after 2 hrs. of incubation. The highest values of progressive motility and movement characteristics after thawing and after 2 hours incubation were detected using slow thawing method. Moreover, the rapid method of thawing showed the significantly the lowest results in progressive motility (−3.6 % to −6.6 %), VSL (−3.2 to −5.6 µm/s) and LIN (−3 % to −4.6 %) characteristics assessed after 2 hrs. of incubation compared the others (P < 0.05). Control method of thawing was most stable during the incubation and showed the significantly lowest decrease of total motility (−7.6 % to −11.8 %, P < 0.05), progressive spermatozoa motility above head (−7.5 % to −9.8 %, P < 0.05) and VSL characteristics (−2.4 to −9.7 µm/s, P < 0.05) during incubation. Therefore, this thawing protocol can be recommended at this study based on the lowest spermatozoa characteristics decline during incubation for using artificial insemination.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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Effect of Different Thawing Methods on Bull's Semen Characteristics

Doležalová

Ptáček

Stádník

et al. 2017

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…The amount of annually produced cryopreserved ID exceeds several hundred millions, whereas production of fresh doses reaches only one-fifth of that number (Gordon, 2017). A standard preservation procedure consists of several different steps, namely, semen collection, evaluation, dilution, cooling, equilibration and freezing-thawing process in case of cryopreservation (Doležalová et al, 2016;Khalil et al, 2018). Both aforementioned ID production methods provide their own advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Pregnancy Rate of Dairy Cows Inseminated with Fresh or Frozen-Thawed Semen

Pytlík¹,

Stádník²,

Ducháček³

et al. 2020

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of this study was to investigate conception rates (CR) achieved with artificial insemination of Holstein cows using fresh or frozen insemination doses (ID). The synchronization protocols (Double-Ovsynch, Ovsynch, ReSynch) and pedometers were used to secure proper timing of artificial inseminations (AI). Total of 3979 inseminations data were recorded. Statistical analysis was performed using the SAS 9.3 package. For the evaluation of a CR, the effects of ID type, season, year, sire breed, lactation number and oestrus detection type, were analysed. Cows inseminated with fresh ID performed higher CR (+4.86%; P < 0.05). Best pregnancy per artificial insemination (P/AI) success was observed during the winter season (+11.82%; P < 0.01). Parity affected P/AI with primiparous having a greater P/AI than multiparous cows (P < 0.01). The least likelihood of pregnancy success matched up with ReSynch and the highest with Ovsynch protocol (+7.7%; P < 0.05). In conclusion, fresh ID manifested superior CR over frozen-thawed semen under common conditions of Czech dairy farming. Similar or better P/AI was achieved when an automated activity monitoring were used rather than timed AI based on Ovsynch protocol, indicating a possible reduction in hormonal treatment applications without affecting fertility of high producing dairy cows.

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“…Semen cooling rate induces irreversible changes in membrane composition and functionality (Watson 2000). Other factors affecting the sperm motility and sperm membrane integrity are time of equilibration, freezing rate, and interactions between equilibration time and freezing rate (Dolezalova et al 2016). Cryopreservation process also affects acrosomal integrity and capacitation (Pons-Rejraji et al 2009).…”

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confidence: 99%

Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

Biniová¹,

Stádník²,

Doležalová³

et al. 2018

Czech J. Anim. Sci.

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The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed.

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Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Cited by 13 publications

References 34 publications

Effect of Different Thawing Methods on Bull's Semen Characteristics

Effect of Different Thawing Methods on Bull's Semen Characteristics

Comparative Study of Pregnancy Rate of Dairy Cows Inseminated with Fresh or Frozen-Thawed Semen

Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

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