2009
DOI: 10.1007/s11120-009-9461-z
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Equilibration kinetics in isolated and membrane-bound photosynthetic reaction centers upon illumination: a method to determine the photoexcitation rate

Abstract: Kinetics of electron transfer, following variation of actinic light intensity, for photosynthetic reaction centers (RCs) of purple bacteria (isolated and membrane-bound) were analyzed by measuring absorbance changes in the primary photoelectron donor absorption band at 865 nm. The bleaching of the primary photoelectron donor absorption band in RCs, following a sudden increase of illumination from the dark to an actinic light intensity of I exp , obeys a simple exponential law with the rate constant ðaI exp þ k… Show more

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Cited by 2 publications
(2 citation statements)
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“…Transient absorption experiments were carried out as described previously . Briefly, absorbance changes of RC samples in a quartz cuvette were probed with a monitoring light beam from a quartz tungsten–halogen (QTH) lamp filtered by monochromators set at 865 nm (slit bandwidth = 20 nm) and placed before and after the cuvette.…”
Section: Methodsmentioning
confidence: 99%
“…Transient absorption experiments were carried out as described previously . Briefly, absorbance changes of RC samples in a quartz cuvette were probed with a monitoring light beam from a quartz tungsten–halogen (QTH) lamp filtered by monochromators set at 865 nm (slit bandwidth = 20 nm) and placed before and after the cuvette.…”
Section: Methodsmentioning
confidence: 99%
“…Accordingly, Manzo et al (2009) have recently proposed a method to measure a based on the use of an additional orthogonal light beam. Alternatively, Eq.…”
Section: Discussionmentioning
confidence: 99%