Equilibrium and kinetics of thermal unfolding of yeast 5.8S ribosomal RNA in aqueous salt solutions

Maruyama, Satoshi; Akazaki, Shuichi; Nitta, Katsutoshi; Sugai, Shintaro

doi:10.1016/0141-8130(83)90074-0

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…In Table 2, AH for the local structure 1 in yeast 5S RNA is shown at I0.03. Activation energy for formation of the structure 1 resulted in a negative value, which indicates the nucleation proceeds for folding of double helical structure 1 in the RNA, as shown for double helical structures in tRNAs (30-32) and yeast 5.8S RNA (23). Values of AH and activation energies of the local structure 1 are reasonable for a double helix to coil transition.…”

Section: (B)mentioning

confidence: 64%

“…Ind.,Ltd., and Nakarai Chem.,Ltd.). Differential thermal melting was obtained at both 260 and 280 nm in a spectrophotometer (Union SM-401), as described previously (23). Heating was performed at a rate of -0.50C minm1 from 10 to 95°C.…”

Section: Methodsmentioning

confidence: 99%

“…The temperature jump measurements were carried out in a rapid reaction analyser (Union RA-410), as described previously (23,24). A temperature jump size of 4.50C was used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…However, the parameters of the structure 1 could be estimated from the kinetic melting curve shown in Fig. 5(A), as shown for yeast 5.8S RNA (23). Scarce dependence of enthalpy change of the transition, AH, on I in its range between 0.03 and 1.0 may be assumed, as for the local structures in yeast 5.8S RNA (23).…”

Section: (B)mentioning

confidence: 99%

“…5(A), as shown for yeast 5.8S RNA (23). Scarce dependence of enthalpy change of the transition, AH, on I in its range between 0.03 and 1.0 may be assumed, as for the local structures in yeast 5.8S RNA (23). In Table 2, AH for the local structure 1 in yeast 5S RNA is shown at I0.03.…”

Section: (B)mentioning

confidence: 99%

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Melting of local ordered structures in yeast 5s ribosomsal RNA in aqueous salts

Ohta¹,

Maruyama²,

Nitta³

et al. 1983

Nucl Acids Res

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Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each of melting temperatures T1m and T2m and log I was found at I=0.01-0.5 in the Mg2+-free solution. The local structures were found to be stabilized considerably by Mg2+. The temperature jump measurements gave the kinetic melting curve of the structure 1 at I=0.03 without Mg2+ or with 0.5 mM Mg2+. The kinetic Tm coincided well with the corresponding static Tm. For the structure 1, various parameters were calculated from the kinetic data, which indicated a double helical character of the structure 1. In terms of the values of Tm, G-C content, and enthalpy change of the transition of the structure 1 or 2, appropriateness of each of the secondary structure models of eukaryotic 5S RNA proposed previously was discussed.

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Section: (B)mentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

“…The temperature jump measurements were carried out in a rapid reaction analyser (Union RA-410), as described previously (23,24). A temperature jump size of 4.50C was used in this study.…”

Section: Methodsmentioning

confidence: 99%

Section: (B)mentioning

confidence: 99%

Section: (B)mentioning

confidence: 99%

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