Equilibrium on reactive extraction of glabridin in a quaternary solvent system containing SBE-β-CD

Wang, Wanru; Yang, Yong; Tang, Kewen

doi:10.1016/j.cherd.2022.05.040

Cited by 3 publications

(2 citation statements)

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“…For the product samples, the glabridin concentration ranged between 0.65 and 7.21 μg/g dry weight. Moreover, many studies developed methods of extraction to increase glabridin yield, such as using a deep eutectic solvent with sonication, 23 using an organic solvent with cyclodextrin derivatives, and varying solvent polarity 24 …”

Section: Resultsmentioning

confidence: 99%

“…Moreover, many studies developed methods of extraction to increase glabridin yield, such as using a deep eutectic solvent with sonication, 23 using an organic solvent with cyclodextrin derivatives, and varying solvent polarity. 24 To compare whether the results of HPLC and ELISA corresponded, Pearson's correlation was equal to 0.985 [95% confidence interval (CI) = 0.951-0.995], indicating highly correlated results.…”

Section: Validation Of the Developed Elisa Methodsmentioning

confidence: 99%

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Construction of a monoclonal antibody against glabridin (2G4) and development of an enzyme‐linked immunosorbent assay

Krittanai,

Choonong,

Butdapheng

et al. 2023

Phytochemical Analysis

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Introduction Glabridin is a unique isoflavonoid found only in Glycyrrhiza glabra L. The pharmacological effects of glabridin are well established, especially for beauty‐ and wellness‐related uses, such as antioxidant, anti‐inflammatory, ultraviolet (UV) protection, and skin‐lightening effects. Therefore, glabridin is often found in commercial products such as creams, lotions, and dietary supplements. Objective This study aimed to develop an enzyme‐linked immunosorbent assay (ELISA) using a glabridin‐specific antibody. Method Immunogen conjugation of glabridin‐bovine serum albumin was performed via the Mannich reaction, and the resulting conjugates were injected into BALB/c mice. Subsequently, hybridomas were produced. An ELISA method for glabridin determination was developed and validated. Result A highly specific antibody against glabridin was produced using clone 2G4. The assay range for the determination of glabridin was 0.28–7.02 μg/ml, with a detection limit of 0.16 μg/ml. The validation parameters in terms of accuracy and precision met the acceptable criteria. Standard curves of glabridin in various matrices were compared to evaluate the matrix effect on human serum using ELISA. Standard curves of the human serum and water matrix were obtained in the same manner, and the measurement range was 0.41–10.57 μg/ml. Conclusion The developed ELISA method was used to quantify glabridin in plant materials and products with high sensitivity and specificity, and has potential applications in quantifying compounds in plant‐derived products and human serum samples.

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Section: Resultsmentioning

confidence: 99%

Section: Validation Of the Developed Elisa Methodsmentioning

confidence: 99%