Equilibrium partitioning of Ficoll in composite hydrogels

Kosto, Kimberly B.; Panuganti, Swapna; Deen, William M.

doi:10.1016/j.jcis.2004.04.063

Cited by 11 publications

(14 citation statements)

References 37 publications

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“…Fluorescence correlation spectroscopy (FCS)[82, 100] and fluorescence recovery after photobleaching (FRAP)[12, 20, 31, 50, 52, 80, 101, 102] have been employed to visualize proteins and peptides, viruses, nano and micro polymeric particles, and flexible polymers such as Ficoll, Dextran and DNA. Saltzman et al reported a study of antibody diffusion in human cervical mucus using epi-fluorescent imaging and FRAP.…”

Section: Measuring Diffusion Coefficients: Materials Methods and mentioning

confidence: 99%

Mathematical modeling of molecular diffusion through mucus

Saltzman

2009

Advanced Drug Delivery Reviews

108

114

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The rate of molecular transport through the mucus gel can be an important determinant of efficacy for therapeutic agents delivered by oral, intranasal, intravaginal/rectal, and intraocular routes. Transport through mucus can be described by mathematical models based on principles of physical chemistry and known characteristics of the mucus gel, its constituents, and of the drug itself. In this paper, we review mathematical models of molecular diffusion in mucus, as well as the techniques commonly used to measure diffusion of solutes in the mucus gel, mucus gel mimics, and mucosal epithelia.

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Section: Measuring Diffusion Coefficients: Materials Methods and mentioning

confidence: 99%

Mathematical modeling of molecular diffusion through mucus

Saltzman

2009

Advanced Drug Delivery Reviews

108

114

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“…But, they considered that the transport of such a small molecule as BSA (7.2 nm) was comparable to nanocarriers of about 20-200 nm in size. The diffusivity of nanoparticles in porous media was reduced when compared to that of small molecules in solution because of a combination of hydrodynamic and steric factors [105,106].…”

Section: Osmolaritymentioning

confidence: 99%

Convection-enhanced delivery of nanocarriers for the treatment of brain tumors

2009

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“…For point solutes, the partition coefficient must reduce to the volume fraction of water in the gel, i.e., k = 1 – φ, where φ is volume fraction of polymer in the gel. − The ratio k /(1 – φ) can be considered an enhancement factor E ≡ k / ( 1 − φ ) Solutes that bind specifically with the polymer chains exhibit enhancement factors significantly greater than unity. These may be thought of as adsorbed onto the polymer chains.…”

Section: Introductionmentioning

confidence: 99%

“…Quantifying mesh size is clearly important in determining how gel matrices absorb solutes and how such impregnated gels function in a variety of applications ranging from drug delivery to separation processes. In many applications, solute size is comparable with the average mesh size of a hydrogel. ,− ,,, In such cases, excluded-volume interactions of the solutes with the gel matrix are crucial.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Solute Partitioning and Mesh Size in HEMA/MAA Hydrogels

et al. 2012

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Using two-photon confocal microscopy, equilibrium partition coefficients, k, were measured for aqueous Na-fluorescein, fluorescently labeled dextrans with molecular masses ranging from 4 to 20 kDa, two fluorescently labeled proteins with opposite charges, anionic bovine serum albumin (BSA), and cationic avidin in anionic 70 wt % hydroxyethyl methacrylate (HEMA)/30 wt % methacrylic acid (MAA) gels saturated with aqueous phosphate buffer solution. Cross-linking density with ethylene glycol–dimethacrylate (EGDMA) ranged from 0 to 1 wt %. All partition coefficients, except for avidin, were considerably less than unity and diminished strongly with increasing Stokes–Einstein diameter of the free aqueous solute. The average mesh size of the wet gels, obtained from the zero-frequency oscillatory shear-storage gel modulus, ranged from 3.6 to 8.3 nm over the cross-link ratios studied. Except for Na-fluorescein, solute hydrodynamic diameters were larger than the smallest average gel mesh size. Yet, all solutes permeated the gels but with small partition coefficients less than about 0.001 for the largest diameter solutes in the small mesh size gels. To express deviation from ideal partitioning, we define an enhancement (or exclusion) factor, E ≡ k/(1 – φ), where φ is the polymer volume fraction in the gel and E is unity for point solutes. A hard-sphere excluded-volume Ogston mesh size distribution is adopted to predict a priori the measured enhancement factors as a function of average gel mesh size for those solutes that do not interact specifically with the anionic gel (i.e., for solutes with E < 1). Agreement between the extended Ogston distribution and experiment is qualitative for both enhancement factors and water content of the gels. The cationic protein, Fl-avidin, exhibits a large enhancement factor in the anionic gels due to strong specific interaction with the charged carboxylate groups of MAA. In this case, consideration must be given to both hard-sphere size exclusion and specific complexation with the polymer strands.

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Equilibrium partitioning of Ficoll in composite hydrogels

Cited by 11 publications

References 37 publications

Mathematical modeling of molecular diffusion through mucus

Mathematical modeling of molecular diffusion through mucus

Convection-enhanced delivery of nanocarriers for the treatment of brain tumors

Aqueous Solute Partitioning and Mesh Size in HEMA/MAA Hydrogels

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