Equine Bone Marrow and Adipose Tissue Mesenchymal Stem Cells: Cytofluorimetric Characterization, In Vitro Differentiation, and Clinical Application

Iacono, Eleonora; Merlo, Barbara; Romagnoli, Noemi; Rossi, Barbara; Ricci, Francesca; Spadari, Alessandro

doi:10.1016/j.jevs.2014.12.010

Cited by 12 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of surface marker expression patterns provided further insights into the characteristics of human MSCs, but only one antibody of the panel available was cross-reacting with equine epitopes. This study confirms what already observed by others in the horse (9,10,29), and adds to the list a new antibody that does not recognize the corresponding equine epitope (CD 19, clone LT-19).…”

Section: Discussionsupporting

confidence: 90%

Wharton’s Jelly Derived Mesenchymal Stem Cells: Comparing Human and Horse

Merlo

Teti

Mazzotti

et al. 2018

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

Wharton's jelly (WJ) is an important source of mesenchymal stem cells (MSCs) both in human and other animals. The aim of this study was to compare human and equine WJMSCs. Human and equine WJMSCs were isolated and cultured using the same protocols and culture media. Cells were characterized by analysing morphology, growth rate, migration and adhesion capability, immunophenotype, differentiation potential and ultrastructure. Results showed that human and equine WJMSCs have similar ultrastructural details connected with intense synthetic and metabolic activity, but differ in growth, migration, adhesion capability and differentiation potential. In fact, at the scratch assay and transwell migration assay, the migration ability of human WJMSCs was higher (P < 0.05) than that of equine cells, while the volume of spheroids obtained after 48 h of culture in hanging drop was larger than the volume of equine ones (P < 0.05), demonstrating a lower cell adhesion ability. This can also revealed in the lower doubling time of equine cells (3.5 ± 2.4 days) as compared to human (6.5 ± 4.3 days) (P < 0.05), and subsequently in the higher number of cell doubling after 44 days of culture observed for the equine (20.3 ± 1.7) as compared to human cells (8.7 ± 2.4) (P < 0.05), and to the higher (P < 0.05) ability to form fibroblast colonies at P3. Even if in both species tri-lineage differentiation was achieved, equine cells showed an higher chondrogenic and osteogenic differentiation ability (P < 0.05). Our findings indicate that, although the ultrastructure demonstrated a staminal phenotype in human and equine WJMSCs, they showed different properties reflecting the different sources of MSCs.

show abstract

Section: Discussionsupporting

confidence: 90%

Wharton’s Jelly Derived Mesenchymal Stem Cells: Comparing Human and Horse

Merlo

Teti

Mazzotti

et al. 2018

Stem Cell Rev and Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…All of our results showed that these cells have the capacity to synthesize a specific protein representative of hyaline articular cartilage in collagen sponges in the presence of chondrogenic factors. The comparison with other studies is not straightforward because other protocols perform chondrocyte differentiation in monolayer cultures [ 52 ], in pellet cultures [ 50 ], or in hydrogels [ 53 ] in the presence of only one growth factor. Equine MSCs derived from adipose tissue cultured in a micromass system at P4 showed only a non-significant induction of type II collagen and aggrecan [ 50 ] and at P2, Alcian blue staining was more intense at the periphery of cell pellets, giving evidence of an incomplete and heterogenous differentiation.…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy

Desancé

Contentin

Bertoni

et al. 2018

IJMS

View full text Add to dashboard Cite

Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair. Here, we characterized equine umbilical cord blood-derived MSCs (eUCB-MSCs) and evaluated their potential for chondrocyte differentiation for use in cartilage repair therapy. Our results show that isolated eUCB-MSCs had high proliferative capacity and differentiated easily into osteoblasts and chondrocytes, but not into adipocytes. A three-dimensional (3D) culture approach with the chondrogenic factors BMP-2 and TGF-β1 potentiated chondrogenic differentiation with a significant increase in cartilage-specific markers at the mRNA level (Col2a1, Acan, Snorc) and the protein level (type II and IIB collagen) without an increase in hypertrophic chondrocyte markers (Col10a1 and Mmp13) in normoxia and in hypoxia. However, these chondrogenic factors caused an increase in type I collagen, which can be reduced using small interfering RNA targeting Col1a2. This study provides robust data on MSCs characterization and demonstrates that eUCB-MSCs have a great potential for cartilage tissue engineering.

show abstract

“…Bone marrow (BM) is the common source of autologous MSCs for clinical applications in equine medicine. Alternatively, adipose tissue-derived MSCs can be used as they have a higher proliferation potential (Iacono et al 2015a). However, for both sources, an invasive procedure is required and a large variability in the cell yield related to the donor was demonstrated (Colleoni et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural characteristics and immune profile of equine MSCs from fetal adnexa

Iacono¹,

Pascucci²,

Rossi³

et al. 2017

Reproduction

Self Cite

View full text Add to dashboard Cite

Both in human and equine species, mesenchymal stem cells (MSCs) from amniotic membrane (AM) and Wharton's jelly (WJ), may be particularly useful for immediate use or in later stages of life, after cryopreservation in cell bank. The aim of this study was to compare equine AM- and WJ-MSCs features that may be relevant for their clinical employment. MSCs were more easily isolated from WJ, even if MSCs derived from AM exhibited more rapid proliferation ( < 0.05). Osteogenic and chondrogenic differentiation were more prominent in MSCs derived from WJ. This is also suggested by the lower adhesion of AM cells, demonstrated by the greater volume of spheroids after hanging drop culture ( < 0.05). Data obtained by PCR confirmed the immunosuppressive function of AM and WJ-MSCs and the presence of active genes specific for anti-inflammatory and angiogenic factors (IL-6, IL 8, IL-β1). For the first time, by means of transmission electron microscopy (TEM), we ascertained that equine WJ-MSCs constitutively contain a very impressive number of large vesicular structures, scattered throughout the cytoplasm. Moreover, an abundant extracellular fibrillar matrix was located in the intercellular spaces among WJ-MSCs. Data recorded in this study reveal that MSCs from different fetal tissues have different characteristics that may drive their therapeutic use. These finding could be noteworthy for horses as well as for other mammalian species, including humans.

show abstract

Equine Bone Marrow and Adipose Tissue Mesenchymal Stem Cells: Cytofluorimetric Characterization, In Vitro Differentiation, and Clinical Application

Cited by 12 publications

References 42 publications

Wharton’s Jelly Derived Mesenchymal Stem Cells: Comparing Human and Horse

Wharton’s Jelly Derived Mesenchymal Stem Cells: Comparing Human and Horse

Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy

Ultrastructural characteristics and immune profile of equine MSCs from fetal adnexa

Contact Info

Product

Resources

About