Equine herpesvirus 1 HVS25A isolated from an aborted foetus produces disease in Balb/C mice

Csellner, H.; Jm, Whalley; Dn, Love

doi:10.1111/j.1751-0813.1995.tb15336.x

Cited by 13 publications

(1 citation statement)

References 11 publications

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“…O desenvolvimento de um modelo murino levou a um considerável número de estudos com o objetivo de investigar a patogênese e a resposta imune do EHV-1 em camundongos (FIELD et al, 1992;FIELD, 1993a,b;INAZU et al, 1993;ALBER et al, 1995;AWAN et al, 1995;BAXI et al, 1995;CSELLNER et al, 1995;FIELD, 1997;BARTELS et al, 1998;SMITH et al, 1998b;WALKER et al, 1998a,b). Do mesmo modo, esse modelo animal tem sido usado para investigar a eficiência de imunógenos frente ao EHV-1 (TEWARI et al, 1994(TEWARI et al, , 1995OSTERRIEDER et al, 1995;STOKES et al, 1996STOKES et al, , 1997KUKREJA et al, 1998a,b;PACKIARAJAH et al, 1998;RUITENBERG et al, 1998), o efeito de antivirais na infecção por EHV-1 (FIELD; GIBSON et al, 1992b;, e a patogenicidade de diferentes estirpes de EHV-1 (VAN WOENSEL et al, 1995;COLLE et al, 1996).…”

Section: Sinais Clínicosunclassified

<b>Avaliação da resposta inflamatória no sistema nervoso central causada pelo herpesvírus equino tipo 1 utilizando um modelo murino de neuroinfecção</b>

Tonietti¹

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Ao meu noivo Lucas Garcia Rios À querida Rebeca (in memoriam) À querida Pérola EPÍGRAFE "Há uma força motriz mais poderosa que o vapor, a eletricidade e a energia atômica: a vontade." Albert Einstein AGRADECIMENTOS A Deus, por iluminar meus caminhos e sempre me fortalecer em todos os momentos de minha vida. Aos meus queridos pais, Amauri Tonietti e Roseli de Oliveira Tonietti, pela amizade, apoio, carinho em todas as fases da minha vida e principalmente pelo exemplo de dignidade e respeito. Ao meu noivo Lucas Garcia Rios, pela paciência, carinho e atenção em todos os momentos. Ao Prof. Dr. Paulo César Maiorka, pela orientação, oportunidade de crescimento, confiança, amizade, conselhos, e grandes ensinamentos, proporcionando a execução deste trabalho.

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Section: Sinais Clínicosunclassified

<b>Avaliação da resposta inflamatória no sistema nervoso central causada pelo herpesvírus equino tipo 1 utilizando um modelo murino de neuroinfecção</b>

Tonietti¹

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Effects of equid herpesvirus 1 (EHV-1) AR8 and HH1 strains on BALB-c mice

et al. 2013

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Here, we used a murine model to describe and compare the pathogenic potential of the Argentinean equid herpesvirus 1 (EHV-1) AR8 strain with the Japanese HH1 reference strain. In AR8-inoculated animals, clinical signs began earlier, but the viremic phase was shorter. Virus isolation and DNA detection in the lungs, liver and spleen were positive for both strains at different times postinfection (pi). Infection foci produced by both strains were immunohistochemically detected in lungs from day 1 to day 4 pi. We conclude that whichever EHV-1 strain is selected to experimentally reproduce the disease, it needs appropriate standardization in order to provide valid conclusions.

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An equine herpesvirus 1 mutant with a lacZ insertion between open reading frames 62 and 63 is replication competent and causes disease in the murine respiratory model

et al. 1998

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An equine herpesvirus 1 (EHV-1) mutant was constructed by inserting a lacZ expression cassette into the intergenic region upstream of gene 62 (glycoprotein L; gL) and downstream of gene 63 (a homologue of the herpes simplex virus transcriptional activator ICP0). The recombinant lacZ62/63-EHV-1 had similar growth kinetics in cell culture to those of the parental wild type (wt) virus, with indistinguishable cytopathic effects and plaque morphology. Reverse transcriptase PCR confirmed that the lacZ insertion did not interfere with transcription of gL and immunoblot analysis indicated there was no modification to late gene expression as monitored by synthesis of EHV-1 glycoproteins C and D. The parental EHV-1 isolate HVS25A used here had almost identical nucleotide sequence to that published for isolate Ab4, in a 1200 bp region surrounding the insert, but lacked a HindIII site corresponding to Ab4 position 109,048. The lacZ62/63-EHV-1 caused respiratory disease in BALB/c mice with clinical signs, histopathology and virus titres in lungs throughout days 1-5 post infection similar to those induced by wt EHV-1. X-gal staining for beta-galactosidase expression in murine lungs clearly demonstrated EHV-1 infection in cells of the bronchiolar epithelium and pulmonary parenchyma, with a peak of infection evident at day 2 post infection, when up to 50% of bronchioles demonstrated blue-staining and thus virus-infected epithelial cells. The construction of this replication competent virus carrying a reporter gene identifies a site for insertion of foreign genes and will facilitate studies on the pathogenesis of EHV-1.

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Equine herpesvirus 1 HVS25A isolated from an aborted foetus produces disease in Balb/C mice

Cited by 13 publications

References 11 publications

<b>Avaliação da resposta inflamatória no sistema nervoso central causada pelo herpesvírus equino tipo 1 utilizando um modelo murino de neuroinfecção</b>

<b>Avaliação da resposta inflamatória no sistema nervoso central causada pelo herpesvírus equino tipo 1 utilizando um modelo murino de neuroinfecção</b>

Effects of equid herpesvirus 1 (EHV-1) AR8 and HH1 strains on BALB-c mice

An equine herpesvirus 1 mutant with a lacZ insertion between open reading frames 62 and 63 is replication competent and causes disease in the murine respiratory model

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