Equine hoof canker: a clinical trial of topical cisplatin chemotherapy

Apprich, Veronika; Licka, Theresia

doi:10.1136/vr.101359

Cited by 6 publications

(6 citation statements)

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“…The success of local cisplatin chemotherapy for equine hoof canker is comparable to the success of intralesional cisplatin chemotherapy for papillomavirus-induced equine sarcoids, which is a well-documented therapy for this disease. 1,7,34,35 The increased expression of PCNA protein in hoof canker–associated keratinocytes is another parallel with symptoms of viral infection, given that in human keratinocytes in vitro, PCNA expression is down-regulated by growth inhibition via transforming growth factor β or calcium-induced differentiation, while in HPV-transformed keratinocytes this effect was reduced and PCNA expression was still high. 8 These observations support the concept of papillomaviruses being involved in the development of equine hoof canker, although the causal association with onset and progression remains to be established.…”

Section: Discussionmentioning

confidence: 99%

“…Hoof canker was diagnosed based on its typical clinical appearance. 1,23,24 Tissue samples were taken from superficial regions of canker affected areas with a hoof knife on the first day of treatment and from deep regions including the dermo-epithelial junction during surgery under regional anesthesia on the following day. Tissue sampling and evaluation, as well as anonymized publication of the data obtained, were carried out with the written consent of the horse owners, given on admission of the horse to the clinic.…”

Section: Sample Collectionmentioning

confidence: 99%

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Equine Hoof Canker: Cell Proliferation and Morphology

et al. 2017

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Hoof canker is described as progressive pododermatitis of the equine hoof with absent epidermal cornification and extensive proliferation of the dermal papillary body; however, in-depth research on the type of proliferative activity has not yet been reported. The aim of the present study was to determine cell-specific proliferation patterns together with morphological analysis of hoof canker tissue.Tissues removed during surgery from 19 horses presented for treatment of canker were compared to similar postmortem tissues of healthy hooves of 10 horses.Morphological alterations visible in light microscopy were assessed semiquantitatively and graded for severity. Proliferative activity was evaluated by means of anti-PCNA (proliferative cell nuclear antigen) and anti-Ki67 immunohistochemistry.Histologically, canker tissue showed five major morphological alterations, the presence of lacunae, vacuoles, giant cells, hemorrhage and inflammation, not seen in control tissue. Also, there was a notable koilocytotic appearance of keratinocytes in canker tissue. Immunohistochemistry revealed increased levels of PCNA protein expression in keratinocytes and fibroblasts of canker tissue compared to control tissue. In control tissue, keratinocytes showed higher levels of Ki67 compared to canker tissue, while the dermal fibroblasts of both groups showed similar levels of Ki67, indicating similar proliferative activity of less than 3% of total dermal fibroblasts.These results demonstrate that, in contrast to previous reports, there is no evidence for increased proliferative activity of the dermal papillary body associated with hoof canker. Increased levels of PCNA protein expression and morphological alterations indicate dysregulation of keratinocyte differentiation constitutes a key event in equine hoof canker development. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 3,9,19,20,28 However, for any of these, a causal association with the development of disease remains to be established. Morphological studies of hoof canker tissues are rare. 24, 26Ballooning of the keratinocytes, mainly in the Stratum germinativum of the frog, has been reported together with the occurrence of perinuclear vacuoles in the cells of deep layers of the Stratum spongiosum; the cells of the corium are covered by a thin layer of defective epidermis, leading to an increased risk of infection and bleeding. 26To our knowledge, the proliferative character of canker has not yet been analyzed in detail. Immunohistochemistry allows characterization of tissue proliferation, as has been shown in a number of potentially metaplastic or neoplastic tissues. 4,1...

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Section: Discussionmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

Equine Hoof Canker: Cell Proliferation and Morphology

et al. 2017

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“…Die Krebstherapie wurde ebenfalls in Betracht gezogen. Apprich und Licka (2011) untersuchten die lokale Anwendung von Cisplatin bei 10 Pferden 11 . Cisplatin ist ein Zytostatikum und hemmt die DNA-Replikation der Zelle.…”

Section: Therapieunclassified

Hufkrebs – Immer wieder aktuell

Sette¹,

Martens²

2023

pferde spiegel

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“…Treatment generally involves surgical debridement of the diseased tissue, topical application of antibiotics, topical application of caustic substances or topical cisplatin chemotherapy. A mix of 10% benzoyl peroxide in acetone and metronidazole powder is frequently applied with some success reported (Jongbloets et al, 2005;Oosterlinck et al, 2011;Apprich & Licka, 2013;Oosterlinck, 2015). Jongbloets et al (2005) raised this (auto-) immune hypothesis with the treatment and cure obtained in a mare that had all four feet affected together with the clinical findings that contributed to this immune reaction hypothesis.…”

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Chronic equine proliferative pododermatitis

Azzolini

Bastos

Barros

2019

Braz. J. Vet. Res. Anim. Sci.

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Chronic equine proliferative pododermatitis (canker) is a chronic hypertrophic disease of the hoof, denoted by an excessive proliferation of the horn-producing tissues, which results from a dyskeratosis of the keratinocytes. It occurs mainly at the frog and sulci regions, but can sometimes also affect the heels, sole, bulbs or hoof wall. The etiology still remains unclear. Infectious agents such as anaerobic bacteria, viruses, spirochetes and fungi have been isolated from diseased tissues, but, moreover, an immune cause has been suggested, possibly related to a genetic predisposition. This report shows a case of a Brazilian Sport Horse, 10 years old, that had been affected on the right hindlimb for more than two years without a concise diagnosis. Success was achieved with the treatment consisting of oral prednisolone and topical Purple Mush®.

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Equine hoof canker: a clinical trial of topical cisplatin chemotherapy

Cited by 6 publications

References 7 publications

Equine Hoof Canker: Cell Proliferation and Morphology

Equine Hoof Canker: Cell Proliferation and Morphology

Hufkrebs – Immer wieder aktuell

Chronic equine proliferative pododermatitis

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