Equine seminal plasma derived lactoferrin regulates binding of polymorphonuclear neutrophils (PMNs) to spermatozoa

Troedsson, M.H.T.; Esteller-Vico, A.; Scoggin, Kirsten E.; Woodward, E.M.; Squires, E.L.; Ball, Barry A.; Maxwell, Herris S.

doi:10.1016/j.jevs.2013.10.029

Cited by 11 publications

(4 citation statements)

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“…The seminal plasma protein, lactoferrin, specifically altered the pro‐inflammatory response (decrease of TNF‐α) in these susceptible mares (Fedorka et al., ) whereas no changes in cytokine response were observed in resistant mares (Fedorka et al., ). Lactoferrin, or a lactoferrin‐associated protein, also increases the phagocytosis of non‐viable spermatozoa by PMNs and may thereby play an important role in the inflammatory response of the uterus post‐breeding (Troedsson et al., ). These recent studies investigating the immune modulating properties of seminal plasma proteins contributes to the clinical observation that the duration of breeding‐induced inflammation is shortened when seminal plasma is included in the insemination dose.…”

Section: The Inflammatory Response To Breedingmentioning

confidence: 99%

Inflammation and fertility in the mare

Christoffersen

Troedsson

2017

Reprod Domestic Animals

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Contents A transient uterine inflammation post‐breeding is a normal physiological reaction in the mare, and it is believed that the inflammatory response is necessary to eliminate bacteria and excess spermatozoa introduced into the uterine lumen. A tight balance between multiple pro‐ and anti‐inflammatory factors is required for resolving the breeding‐induced inflammation within 24–36 hr in the reproductively healthy mare, whereas a subpopulation of mares is susceptible to development of a persistent infection that can interfere with fertility. The aetiology of persistent endometritis can be either bacterial or semen‐induced and both scenarios can threaten the establishment of pregnancy. Several factors associated with susceptibility to persistent endometritis have been identified including altered innate immune response in the early inflammatory process, reduced myometrial contractions and impaired opsonization; however, the pathogenesis to susceptibility has not been fully elucidated. Current research focuses on the initial hours of uterine inflammatory responses to semen and bacteria, and potential treatments to modify this altered innate immune response. An increased understanding of the mechanisms involved in the disease progression is necessary to improve the treatment and management of these mares. This review attempts to summarize the current knowledge of the uterine inflammatory and immunological responses to breeding‐induced endometritis, persistent breeding‐induced endometritis (PBIE) and bacterial endometritis in the mare.

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Section: The Inflammatory Response To Breedingmentioning

confidence: 99%

Inflammation and fertility in the mare

Christoffersen

Troedsson

2017

Reprod Domestic Animals

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“…These mares also have increased neutrophilic migration into the lumen of the uterus at 48 h post‐breeding in comparison with resistant mares (Watson & Thomson, ). Lactoferrin is thought to act as a first line of defence in the innate immune response, mediating both of these factors, the expression of cytokines and neutrophil recruitment and signalling (Li et al., ; Troedsson et al., ). In the current study, the addition of exogenous lactoferrin to the insemination dose significantly suppressed the mRNA expression of the pro‐inflammatory cytokine TNF in comparison with the negative control of LRS, as well as the treatment of CRISP‐3.…”

Section: Discussionmentioning

confidence: 99%

“…Lactoferrin also modulates the innate immune response in other tissues by suppression of the pro‐inflammatory cytokines TNF and granulocyte‐macrophage colony‐stimulating factor, as well as the inflammatory‐modulating cytokine IL‐6 (Crouch, Slater, & Fletcher, ; Li et al., ; Machnicki, Zimecki, & Zagulski, ; Slater & Fletcher, ). Lactoferrin promotes the binding of PMNs to non‐viable spermatozoa in vitro , possibly contributing to their digestion and elimination (Troedsson et al., ). Our research appears to be the first to examine the effects of exogenous lactoferrin on the innate immune response in the uterus of susceptible mares.…”

Section: Discussionmentioning

confidence: 99%

“…Seminal plasma also modulates the binding of PMNs to spermatozoa, and recently, the specific proteins involved in this interaction were elucidated. Cysteine‐rich secretory protein‐3 (CRISP‐3) was found to suppress the binding of PMNs to live sperm, while lactoferrin appears to increase the number of dead sperm bound to PMNs in vitro (Doty et al., ; Troedsson et al., ). However, the mechanism by which this occurs is unknown.…”

Section: Introductionmentioning

confidence: 99%

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The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating‐induced endometritis

Fedorka

Scoggin

Woodward

et al. 2016

Reprod Domestic Animals

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In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating-induced endometritis PMIE Select seminal plasma proteins cysteine-rich secretory protein-3 (CRISP-3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP-3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Six mares classified as susceptible to PMIE were inseminated during four consecutive oestrous cycles with treatments in randomized order of: 1 mg/ml CRISP-3, 150 μg/ml lactoferrin, seminal plasma (positive control) or lactated Ringer's solution (LRS; negative control) to a total volume of 10 ml combined with 1 × 10 spermatozoa pooled from two stallions. Six hours after treatment, an endometrial biopsy was obtained for qPCR analysis of selected genes associated with inflammation (pro-inflammatory cytokines interleukin (IL)-1β, IL-8, tumour necrosis factor (TNF)-α, interferon (INF)-γ, anti-inflammatory cytokines IL-1RN and IL-10, and inflammatory-modulating cytokine IL-6). Seminal plasma treatment increased the mRNA expression of IL-1β (p = .019) and IL-8 (p = .0068), while suppressing the mRNA expression of TNF (p = .0013). Lactoferrin also suppressed the mRNA expression of TNF (p = .0013). In conclusion, exogenous lactoferrin may be considered as one modulator of the complex series of events resulting in the poorly regulated pro-inflammatory response seen in susceptible mares.

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