Equine Tight Junctions: Tissue-Specific Localization and Expression of Junction Adhesion Molecule-A, Zona Occludens-1, and Occludin

Kang, Hee Young; Lee, Bonn; Lee, Dong‐Oh; Kim, Kipung; Jung, Jun Mo; Ahn, Changhwan; Jeung, Eui‐Bae

doi:10.1016/j.jevs.2016.07.004

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…We identified that 69 signalling pathways were significantly activated ( p < 0.05) with Diana Tools mirPath software (Vlachos et al., 2015 ). The pathways remain diverse, but many of them, such as the TGF‐beta signalling pathway, lysine degradation, ECM‐receptor interactions, adherent junctions and focal adhesions, are involved in the control of normal and OA joints (Kang et al., 2016 ; Mok & Urschel, 2020 ; Shen et al., 2014 ; Van Der Kraan, 2018 ). Other pathways and their roles, including mTOR and HIF‐1 signalling pathways, have been investigated in exercised and sepsis‐related laminitis equine subjects, respectively (Hauss et al., 2021 ; Pawlak et al., 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery

Reynolds

Pan

et al. 2023

J of Extracellular Bio

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The use of mesenchymal stem cells (MSCs) in human and veterinary clinical applications has become a subject of increasing importance due to their roles in immunomodulation and regenerative processes. MSCs are especially relevant in equine medicine because they may have the ability to treat prevalent musculoskeletal disorders, among other conditions. However, recent evidence suggests that the components secreted by MSCs, particularly extracellular vesicles (EVs), are responsible for these properties. EVs contain proteins and nucleic acids, which possess an active role in intercellular communication and can be used as therapeutics. However, because the intersection of equine veterinary medicine with EVs remains a relatively new field, there is a demand to identify biomarkers that can discern and enrich for therapeutic EVs, progressing their clinical efficacy. In this study, we identified and characterized 84 miRNAs, between three equine donors involved in immunomodulation in cell and EV subjects. We discovered distinct groups of shared miRNAs, like miR-21-5p and miR-451a, that are abundant and enriched between the donors' EVs, respectively. By mapping and comparing the MSC-EV miRNA expression, we discovered many pathways that are involved in immunomodulation and tissue regenerative processes related to equine clinical applications. Therefore, the miRNAs highlighted in this article can be used as valuable biomarkers for screening MSC-derived EVs for potential equine therapy.

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Section: Resultsmentioning

confidence: 99%

Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery

Reynolds

Pan

et al. 2023

J of Extracellular Bio

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“…This resistance is influenced by several factors, such as astrocytes ( 142 ), the viscosity of the culture medium, and electrode type ( 143 ). BMECs express high levels of claudin-5 and occludin, involved in tight junction formation ( 144 ); ZO-1, a tight junction protein ( 145 ); GLUT-1, a glucose transporter; and p-glycoprotein, an efflux transporter.…”

Section: Evolution Of Vascularized Tissues and Organoidsmentioning

confidence: 99%

Blood vessels in a dish: the evolution, challenges, and potential of vascularized tissues and organoids

Nwokoye,

Abilez

2024

Front. Cardiovasc. Med.

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Vascular pathologies are prevalent in a broad spectrum of diseases, necessitating a deeper understanding of vascular biology, particularly in overcoming the oxygen and nutrient diffusion limit in tissue constructs. The evolution of vascularized tissues signifies a convergence of multiple scientific disciplines, encompassing the differentiation of human pluripotent stem cells (hPSCs) into vascular cells, the development of advanced three-dimensional (3D) bioprinting techniques, and the refinement of bioinks. These technologies are instrumental in creating intricate vascular networks essential for tissue viability, especially in thick, complex constructs. This review provides broad perspectives on the past, current state, and advancements in key areas, including the differentiation of hPSCs into specific vascular lineages, the potential and challenges of 3D bioprinting methods, and the role of innovative bioinks mimicking the native extracellular matrix. We also explore the integration of biophysical cues in vascularized tissues in vitro, highlighting their importance in stimulating vessel maturation and functionality. In this review, we aim to synthesize these diverse yet interconnected domains, offering a broad, multidisciplinary perspective on tissue vascularization. Advancements in this field will help address the global organ shortage and transform patient care.

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“…As well known, a healthy intestinal tract is crucial for the growth performance and health of fish. The intestinal, mechanical, and immunologic barriers are the primary line of defense against pathogen infection [20][21][22][23]. Moreover, a beneficial intestinal microbiota is essential for maintaining the health of the intestine and host.…”

Section: Introductionmentioning

confidence: 99%

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

Zhao

Pan

Zhang

et al. 2023

Aquaculture Nutrition

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A 12-week feeding trial was conducted to investigate the effect of the same fishmeal protein level replaced by black soldier fly larvae (Hermetia illucens) meal (BSFL) with different lipid contents on the growth performance and intestinal health of juvenile turbot (Scophthalmus maximus L.) (initial body weight 12.64 g). Three isonitrogenous and isolipidic diets were formulated: fish meal-based diet (FM), diets DF and FF, in which 14% fish meal protein of the FM diet was replaced by defatted and full-fat BSFL, respectively. There were no significant differences in growth performance, intestinal morphology, and mucosal barrier function between the DF and the FM group. However, diet FF markedly reduced the growth performance, intestinal perimeter ratio, and the gene expression of anti-inflammatory cytokine TGF-β ( P < 0.05 ). Compared to group FF, the communities of intestinal microbiota in group DF were more similar to group FM. Moreover, diet DF decreased the abundance of some potential pathogenic bacteria and enriched the potential probiotics, such as Bacillus. Diet FF obviously altered the composition of intestinal microbiota and increased the abundance of some potential pathogenic bacteria. These results suggested that the application of defatted BSFL showed more positive effects on fish growth and intestinal health than the full-fat BSFL, and the intestinal microbiota was closely involved in these effects.

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Equine Tight Junctions: Tissue-Specific Localization and Expression of Junction Adhesion Molecule-A, Zona Occludens-1, and Occludin

Cited by 3 publications

References 33 publications

Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery

Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery

Blood vessels in a dish: the evolution, challenges, and potential of vascularized tissues and organoids

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

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