Equine versus Bovine Pericardium in Transmeatal Underlay Myringoplasty

Albera, Roberto; Dagna, Federico; Lacilla, Michelangelo; Canale, Andrea

doi:10.1177/000348940911800409

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…Decellularized xenogeneic pericardia of different species [18,19,21,30], preferentially bovine, have been demonstrated to offer similar advantages and overcome the restrictions of human and GA-treated heterologous tissues in reconstructive or substitutive surgery [22][23][24][25][26][27][28][29]. Yet, no comparative studies have thoroughly investigated the decellularization effects on tissues from different species.…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, the clinical experience with heterologous acellular pericardium is still restricted, with few indications being available on the real long-term performance. Xenogeneic commercial products, as Tutopatch TM [18], Cova TM [19], Jason® [8], Integra® [20], and Osteokor pericardia [21], were released to treat several defects, requiring dura mater plastics, myringoplasty, digestive tissue reconstructions, bone and dental surgeries, cardiac corrections, or soft tissue augmentation to ameliorate facial and breast esthetics [22][23][24][25][26][27][28][29]. To prevent zoonosis risks, as Creutzfeldt-Jacobs disease, bovine tissues are derived from prion-free certified herd.…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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“…In a hypothetical biocompatibility classification, this assessment puts the EP tissue well behind the bovine and porcine pericardium for high pressure cardiovascular and orthopaedic purposes. Conversely good results were obtained for venous conduits reconstruction [65], treatment of diabetic foot wounds [62] and tympanic perforation [66].…”

Section: Equine Pericardiummentioning

confidence: 99%

Biocompatibility Evaluation Criteria for Novel Xenograft Materials: Distribution and Quantification of Remnant Nucleic Acid and Alpha-Gal Epitope

Naso¹,

Andrea²,

Iop³

2013

J Stem Cell Res Ther

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Objective: Commercially decellularized xenogeneic scaffolds are currently employed as for the healing of diseased tissues. Strangely enough their use is permitted even in absence of any assessment of the elimination of xenogeneic cell material, as the alpha-Gal epitopes. In addition, the decellularization procedures are not monitored to prove the elimination of the calcific potential associated to the nucleic acids remnants. The currently treatment with glutaraldehyde is unable to grant a complete immuno-tolerance of implanted xenogeneic tissues, reducing but not eliminating the immunogenicity particularly for the alpha-Gal epitope (the major hindrance for the success of xenotransplantation). Recently, our group has extensively reported studies focused on the evaluation of biocompatibility properties of xenogeneic tissues. In this report we are performing this investigation and nucleic acid detection to novel xenogeneic tissues that have shown very promising preclinical/clinical results in different areas of application. Methods:The alpha-Gal quantification was conducted by an ELISA test previously developed and patented by our research team which involves the use of the monoclonal anti apha-Gal antibody M86. Immunofluorescence analysis was performed for the visual distribution of both xenogeneic epitopes and nucleic acids residues. Finally for the total DNA quantification a commercially available kit was adopted.Results: While the amount and distribution of the alpha-Gal epitopes was found different between the investigated biomaterials, the presence of nucleic acid remnants has been revealed as common feature, even in those tissues delivered as acellular by the manufacturer. Conclusion:Insufficient quantitative evaluations performed at preclinical level about the residual content of xenogeneic epitopes, detergents and nucleic acid materials in scaffolds have led to disappointing and disastrous results. The risk of these dramatic accidents reoccurring remains very high unless safety parameters, among which the complete removal of major xenogeneic determinants (alpha-Gal) and calcification-prone nucleic acid residues, are identified and introduced in manufacturing practices.

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“…A utilização de malha sintética mostrou-se efetiva com excelentes resultados, mas o custo do material é um dos fatores limitantes para o seu uso corriqueiro em medicina veterinária 4 . O uso do pericárdio equino na forma de implantes são mencionados por vários autores, sobretudo na medicina humana 15,16,17,18 . Resultados positivos também foram obtidos quando se empregou peritônio bovino conservado em glicerina a 98% 19,20 , tendo como principal complicação pós-operatória, a infecção no sítio cirúrgico 5 A glicerina a 98% tem sido utilizada na esterilização e conservação das membranas biológicas 20,21,22,23 , por reduzir a antigenicidade, preservar a textura das peças e apresenta poder antisséptico com amplo espectro de ação, com exceção das formas bacterianas esporuladas 21,24,25,26 .…”

Section: Evaluation Of Perineal Hernia Surgical Treatment In Dogs Witunclassified

Avaliação do tratamento cirúrgico da hérnia perineal em cães com o reforço de membrana de pericárdio equino preservado em glicerina a 98%

Zerwes

Stopiglia

Matera

et al. 2011

Braz. J. Vet. Res. Anim. Sci.

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A utilização de membrana biológica constitui-se método alternativo na reparação de hérnia perineal, e avaliar sua eficiência como reforço após a herniorrafia pela técnica de elevação do músculo obturador interno consiste no objetivo deste trabalho. Para tanto, utilizaram-se dez cães atendidos no Serviço de Cirurgia de Pequenos Animais do Hospital Veterinário da FMVZ/USP. Os animais foram distribuídos em dois grupos, GI e GII, com cinco representantes em cada um. A hérnia perineal foi corrigida pela técnica de elevação do músculo obturador interno em ambos os grupos, sendo que o grupo GII ganhou reforço no diafragma pélvico com enxerto de retalho de pericárdio equino Puro Sangue Inglês, conservado em glicerina a 98%. Os animais foram avaliados por até 90 dias de pós-operatório, mediante análises clínica, ultrassonográfica e radiográfica da região perineal. Os resultados obtidos demonstraram que houve reforço no local da herniorrafia ao se fixar o pericárdio de equino conservado em glicerina a 98%, diminuindo a ocorrência de deiscência de pontos e ruptura do diafragma operado.

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Equine versus Bovine Pericardium in Transmeatal Underlay Myringoplasty

Cited by 13 publications

References 11 publications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

Biocompatibility Evaluation Criteria for Novel Xenograft Materials: Distribution and Quantification of Remnant Nucleic Acid and Alpha-Gal Epitope

Avaliação do tratamento cirúrgico da hérnia perineal em cães com o reforço de membrana de pericárdio equino preservado em glicerina a 98%

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