Equipment for investigations of biological nanostructures by diffraction methods using synchrotron radiation

Investigation of the Initial Crystallization Stage in Lysozyme Solutions by Small-Angle X-ray Scattering

“…SAXS Measurements. SAXS experiments were performed on the DICSY synchrotron beamline (NRC Kurchatov Institute, Moscow) 28 at a constant wavelength of 1.62 Å. SAXS patterns were obtained using a Pilatus3 1M detector with a sample-to-detector distance of 300 mm and an exposure time of 5 min. The primary beam was collimated using a three-slit scheme, which provided a cross-sectional beam size of approximately 0.4 × 0.6 mm 2 on the sample.…”

Instrumental and methodical approaches to the study of the transformation of nanostructural parameters in biological objects

“…X ray diffraction stations were equipped with vari ous systems of detection: fast response one coordi nate detectors (9) [16,22]; Image Plate memory V mo X V mi X screens with the corresponding analyzer (10); and two coordinate detectors (11) with cooled CCD matrices, where the images from the X ray fluorescent screens were transferred with the use of optics [22] or fiber optics [23].…”

“…Several versions of this procedure were designed with the set of the pro grams of experimental control and data gathering and processing. In subsequent years, its modernization on various SR sources was performed [16,22]. The exper iments were conducted both at the dynamic and static mode looking through the development of the response of objects over time on various physicochem ical actions.…”

“…In Fig. 3, two X ray patterns from sil ver behenate (C 22 percent intensity ratio of the peaks. The localization of the reflections in the positional respect for the left part of the image is close to 100, 65, 49, 35, and 33% at the dynamic range above 60 dB.…”

“…The development of instrumentation allowed us to create X ray diffraction stations at the operating chan nels of SR sources [10][11][12][13][14][15][16][17][18][19][20][21][22][23] designed for the following experiments: study of the structure and structural dynamics of skeletal muscle and epithelial tissues in normal and pathological states; study of the natural and engineering silk constructions; and the effect of the high frequency electrosurgical welding (HF weld ing) of biological tissues.…”