Equisetin biosynthesis in Fusarium heterosporum

Sims, James; Fillmore, John P.; Warner, Douglas D.; Schmidt, Eric W.

doi:10.1039/b413523g

Cited by 154 publications

(167 citation statements)

References 20 publications

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“…Fusion of the cytochrome P450 monooxygenase and NADPH-dependent cytochrome reductase functions in a single protein has also been found in the FUM6 involved in fumonisin biosynthesis in F. verticilloides (Seo et al, 2001). The EQIS containing a PKS-nonribosomal peptide synthase (NRPS) hybrid was found to be involved in equisetin biosynthesis in Fusarium heterosporum (Sims et al, 2005). Similarly, the alt5 gene encoding a polyketide synthase also contains a C-methyltransferase domain required for decaketide alternapyrone biosynthesis in Alternaria solani (Fujii et al, 2005).…”

Section: Discussionmentioning

confidence: 90%

The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin toxin biosynthesis

Dekkers

You

Gowda

et al. 2007

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 90%

The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin toxin biosynthesis

Dekkers

You

Gowda

et al. 2007

Fungal Genetics and Biology

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“…[54] Umgekehrt ist er am C-Terminus lokalisiert, um das Polyketid freizusetzen und z. B. in Tetramsäuren [165] und Pyridone umzuwandeln. [148,166,167] …”

Section: Alternative Verlängerungseinheiten Und Andere Bausteineunclassified

Die biosynthetische Grundlage der Polyketid‐Vielfalt

Hertweck

2009

Angewandte Chemie

205

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Molekulares Lego: Polyketide bilden eine äußerst vielfältige Gruppe von Naturstoffen mit faszinierenden Kohlenstoffgerüsten (siehe Beispiele), die aus einfachen Acyl‐Bausteinen synthetisiert werden. Das Zusammenspiel von Chemie, Biochemie und Genetik lieferte wichtige Einblicke in die Programmierung des Polyketid‐Aufbaus und die beteiligten, komplexen Enzymsysteme. Dieser Aufsatz behandelt aktuelle Entwicklungen auf diesem Forschungsgebiet.magnified imagePolyketide bilden eine der größten Naturstoffklassen. Viele dieser Verbindungen – oder Derivate davon – sind wichtige Therapeutika, aber viele sind auch berüchtigte, Lebensmittel verderbende Toxine oder Virulenzfaktoren. Besonders bemerkenswert ist, dass sich die Verbindungen dieser heterogenen Gruppe, zu denen Polyether, Polyene, Polyphenole, Makrolide und Endiine gehören, hauptsächlich von einem der einfachsten natürlichen Bausteine ableiten: Essigsäure. Chemische, genetische und biochemische Untersuchungen haben Aufschluss über die Biosyntheseprogramme gegeben, die zur enormen Strukturvielfalt von Polyketiden führen. Dieser Aufsatz behandelt kürzlich aufgeklärte Biosynthesemechanismen, nach denen höchst unterschiedliche und komplexe Moleküle synthetisiert werden.

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“…1) have the CON-AMP-PP (nonribosomal peptide synthetase [NRPS] module) in addition to the common PKS domains, except for AtLovB and PcMlcA, the PKSs for the statin-like compounds. Nevertheless, these two PKSs have CON and the N terminus of the AMP domains (38). Here, we isolated 38 potential PKS-NRPS hybrids that were clustered with a high bootstrap support (96%) in reducing clade II ( Fig.…”

Section: Vol 75 2009 Insect-specific Pkss In Tropical Fungi 3727mentioning

confidence: 94%

Insect-Specific Polyketide Synthases (PKSs), Potential PKS-Nonribosomal Peptide Synthetase Hybrids, and Novel PKS Clades in Tropical Fungi

Amnuaykanjanasin

Phonghanpot

Sengpanich

et al. 2009

Appl Environ Microbiol

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Polyketides draw much attention because of their potential use in pharmaceutical and biotechnological applications. This study identifies an abundant pool of polyketide synthase (PKS) genes from local isolates of tropical fungi found in Thailand in three different ecological niches: insect pathogens, marine inhabitants, and lichen mutualists. We detected 149 PKS genes from 48 fungi using PCR with PKS-specific degenerate primers. We identified and classified 283 additional PKS genes from 13 fungal genomes. Phylogenetic analysis of all these PKS sequences the comprising ketosynthase (KS) conserved region and the KS-acyltransferase interdomain region yielded results very similar to those for phylogenies of the KS domain and suggested a number of remarkable points. (i) Twelve PKS genes amplified from 12 different insect-pathogenic fungi form a tight cluster, although along with two PKS genes extracted from genomes of Aspergillus niger and Aspergillus terreus, in reducing clade III. Some of these insect-specific fungal PKSs are nearly identical. (ii) We identified 38 new PKS-nonribosomal peptide synthetase hybrid genes in reducing clade II. (iii) Four distinct clades were discovered with more than 75% bootstrap support. We propose to designate the novel clade D1 with 100% bootstrap support "reducing clade V." The newly cloned PKS genes from these tropical fungi should provide useful and diverse genetic resources for future research on the characterization of polyketide compounds synthesized by these enzymes.

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Equisetin biosynthesis in Fusarium heterosporum

Abstract: An iterative polyketide synthase-peptide synthetase hybrid assembles the HIV-1 integrase inhibitory tetramic acid, equisetin, in the filamentous fungus Fusarium heterosporum.

Cited by 154 publications

References 20 publications

The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin toxin biosynthesis

The Cercospora nicotianae gene encoding dual O-methyltransferase and FAD-dependent monooxygenase domains mediates cercosporin toxin biosynthesis

Die biosynthetische Grundlage der Polyketid‐Vielfalt

Insect-Specific Polyketide Synthases (PKSs), Potential PKS-Nonribosomal Peptide Synthetase Hybrids, and Novel PKS Clades in Tropical Fungi

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