Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells

Gründemann, Carsten; Lengen, Karin; Sauer, Barbara; Garcia-Käufer, Manuel; Zehl, Martin; Huber, Roman

doi:10.1186/1472-6882-14-283

Cited by 46 publications

(32 citation statements)

References 27 publications

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“…In addition, this study examined if soluble silica has anti-inflammatory properties by analyzing several parameters that are crucial for the activation and function of lymphocytes. Results are in line with our previous study [17] and further demonstrate that suppression of human immune cell function by horsetail preparations, pictured by an inhibition of proliferation and activation of human lymphocytes via an IL-2-dependent mechanism, is dependent on the silica concentration of the horsetail preparation. Although an increase in the number of apoptotic cells was measurable at high concentrations, retardation of T cell proliferation was not solely based on apoptosis induction since effects were still measurable at lower concentrations.…”

Section: Discussionsupporting

confidence: 92%

“…Therein, fields of internal application are defined for inflammatory diseases of the urinary tract and external application for the treatment of poorly healing wounds. The additional putative medicinal properties are supported by a variety of studies that found diuretic [6], antibacterial [7][8][9], and antioxidant effects [10][11][12][13][14] as well as immunomodulatory properties for the treatment of immune dysfunctions [15][16][17]. We assumed that the medicinal properties of horsetail are at least partly mediated by the high silica content, as it can amount to up to 25 % of the dry weight of the plant [3].…”

Section: CDmentioning

confidence: 98%

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In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica

et al. 2017

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, known as common horsetail, is used for the treatment of inflammatory diseases and is the plant with the highest concentration of silica. Yet it is unknown if the medicinal properties are mediated by its silica content. In the current study, optimal conditions for silica-rich horsetail preparations were identified. Bioactivity of the preparations was analyzed using flow cytometry-based activity and functionality profiling of primary human lymphocytes as well as cytokine measurement using a classical ELISA technique. Experiments revealed that horsetail preparations suppress activation and proliferation of lymphocytes by an interleukin-2-dependent mechanism. The effect increased with the silica concentration in the decoctions. Lymphocytes' polyfunctionality was also influenced, shown by a downregulation of IFN-. Analytical profiling by HPLC-UV-MS and bioactivity testing revealed relevant immunosuppressive concentrations of a component that has been identified as isoquercitrin. Our results show that both silica and isoquercitrin are active compounds of horsetail preparations.

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Section: Discussionsupporting

confidence: 92%

Section: CDmentioning

confidence: 98%

In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica

et al. 2017

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“…Equisetum arvense , native to the Arctic and temperate regions of Europe, belongs to the Equisetopsida family, and it is commonly used as a herbal remedy. The phytotherapic properties are anti‐bacterial (Bessa Pereira et al., ; Milovanović, Radulović, Todorović, Stanković, & Stojanović, ), antioxidant (Cetojević‐Simin et al., ; Wu et al., ) and anti‐inflammatory (Gründemann et al., ).…”

Section: Introductionmentioning

confidence: 99%

Effects of a nutritional supplement in dogs affected by osteoarthritis

Musco

Vassalotti²,

Mastellone

et al. 2019

Veterinary Medicine & Sci

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Osteoarthritis is a form of chronic joint inflammation caused by the deterioration of the joint cartilage, accompanied by chronic pain, lameness and stiffness, particularly after prolonged activity. Alternative treatments of canine osteoarthritis would be desirable and, recently nutraceuticals, have been proposed for this purpose. Twenty cross breed adult dogs affected by osteoarthritis were enrolled and equally divided into two groups (control vs. experimental). The nutritional supplement (Dynamopet srl, Verone, Italy) was administered for 90 days to the dogs of the experimental group in order to evaluate its metabolic and locomotor effects. All the clinical signs (lameness, pain on manipulation and palpation, range of motion and joint swelling) significantly (p < 0.01) improved during the trial as regards the experimental group. This group showed a significantly lower joint score than the control group (mean value 7.40 vs. 3.80). With regard to haematology, the mean corpuscular volume resulted significantly (p < 0.01) higher in the experimental group, i.e. alkaline phosphatase, cholesterol and triglycerides values decreased and were significantly (p < 0.01) lower than the control one, thus suggesting an improvement in bone remodelling and lipid metabolism. A decrease in the reactive oxygen metabolites and an increase in the biological antioxidant potential demonstrated an improvement in oxidative stress during the trial in the experimental group compare to the control group. Interleukins 6 decreased in the experimental group, while interleukins 10 resulted in the opposite trend. Moreover, the administration of up to 3 months of the studied supplement was well tolerated in the dogs and caused no adverse effects.

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“…The activation state of T lymphocytes was determined via cellsurface analysis of CD25 and CD69, as previously reported (Gründemann et al, 2014). Briefly, cells were treated for 24 h. Then, cells were washed with PBS and stained with PE-labeled, anti-CD25 mAbs; FITC-labeled, anti-CD69; and, for the differentiation of CD4 + and CD4 -T cells, with APC-labeled, anti-CD4 mAbs (all from eBioscience, Frankfurt, Germany) for 20 min at 4°C.…”

Section: Analysis Of Activation Marker Of T Cellsmentioning

confidence: 99%

Immunosuppressive Activity of Artemisia argyi Extract and Isolated Compounds

et al. 2020

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The need for novel drugs for the treatment of autoimmune diseases is high, since available pharmaceuticals often have substantial side effects and limited efficacy. Natural products are a good starting point in the development of immunosuppressive leads. Since enhanced T cell proliferation is a common feature of autoimmune diseases, we investigated the T cell proliferation inhibitory potential of an extract library of plants used in traditional Chinese medicine. Using a newly established cell-based screening platform, an ethyl acetate extract of Artemisia argyi H.Lév. & Vaniot (Asteraceae, A. argyi) was found to suppress the proliferation of human primary T lymphocytes in vitro in an IL-2-dependent manner. Flow cytometry-and ELISA-based techniques further demonstrated that the A. argyi extract reduced the activation and function of T cells. Transcription factor analysis and flow cytometric calcium influx investigations indicated that the immunomodulatory effect was based on specific modification of T cell signaling in a non-cytotoxic manner which is mediated via the NFAT pathway and a non-sequestrant inhibition of the calcium influx. A series of guaianolide and seco-guaianolide sesquiterpene lactones, as well as a flavonoid, were identified in a previous study as the bioactive compounds in the A. argyi extract. The effects of these bioactive compounds were compared to those of the crude extract. The tested sesquiterpene lactones act via the transcription factor NFAT and NF-kB, thereby exhibiting their immunosuppressive potential, but have an overall effect on T cell biology on a more-downstream level than the crude A. argyi extract.

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Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells

Cited by 46 publications

References 27 publications

In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica

In Vitro Anti-inflammatory Effects of Equisetum arvense Are Not Solely Mediated by Silica

Effects of a nutritional supplement in dogs affected by osteoarthritis

Immunosuppressive Activity of Artemisia argyi Extract and Isolated Compounds

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