Equol promotes rat osteoblast proliferation and differentiation through activating estrogen receptor

Wang, J.; Xu, Jianzhong; Wang, B.; Shu, Furong; Chen, K.; Mi, Mantian

doi:10.4238/2014.july.4.21

Cited by 31 publications

(17 citation statements)

References 36 publications

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“…Setchell et al [ 26 ] report that S-equol, but not R-equol, has a relatively high affinity for ERs. Furthermore, Wang et al [ 27 ] report that equol enhances ALP activity via ERs in primary osteoblasts. Because we used the racemic form of equol, the effect of equol on osteoblast differentiation may be attenuated by its form.…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Nishide

Tousen

Miki

et al. 2015

IJERPH

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Soy isoflavones, genistein, daidzein and its metabolite equol, as well as β-carotene have been reported to be effective for maintaining bone health. However, it remains to be elucidated whether combining soy isoflavones with β-carotene is beneficial to bone formation. This study investigated the combined effect of soy isoflavones and β-carotene on the differentiation of MC3T3-E1 preosteoblastic cells. Daidzein and genistein alone did not affect cell growth but increased alkaline phosphatase (ALP) activity. Beta-carotene alone inhibited cell growth and markedly enhanced ALP activity. Soy isoflavones combined with β-carotene resulted in higher ALP activity than treatment with isoflavones or β-carotene alone. We observed significant main effects of β-carotene on the enhanced expression of Runx2, ALP, and ostepontin mRNA, whereas there was a significant main effect of soy isoflavones on the expression of osterix mRNA. To investigate how β-carotene affected osteoblast differentiation, MC3T3-E1 cells were treated with retinoic acid receptor (RAR) pan-antagonist combined with β-carotene. Osteopontin and ALP mRNA expression levels, which were increased following treatment with β-carotene, were significantly suppressed by the RAR pan-antagonist. This suggests treatment with β-carotene enhanced early osteoblastic differentiation, at least in part via RAR signaling. These results indicate that a combination of isoflavones and β-carotene may be useful for maintaining a positive balance of bone turnover by inducing osteoblast differentiation.

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Section: Discussionmentioning

confidence: 99%

Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Nishide

Tousen

Miki

et al. 2015

IJERPH

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“…Moreover, in addition to its carcinogenic potential, TPA also promoted resistance to tamoxifen induced apoptosis (Luciani-Torres et al, 2015). These chemicals were found also in animal foodstuff, with most samples showing significant estrogenic activities and high BPA contents (Wang et al, 2014). …”

Section: Synthetic Xenoestrogens: Endocrine Disrupting Chemicals (mentioning

confidence: 99%

Risks and benefits related to alimentary exposure to xenoestrogens

Paterni

Granchi

Minutolo

2017

Critical Reviews in Food Science and Nutrition

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Xenoestrogens are widely diffused in the environment and in food, thus a large portions of human population is worldwide exposed to them. Among alimentary xenoestrogens, phytoestrogens (PhyEs) are increasingly being consumed because of their potential health benefits, although there are also important risks associated to their ingestion. Furthermore, other xenoestrogens that may be present in food are represented by other chemicals possessing estrogenic activities, that are commonly defined as endocrine disrupting chemicals (EDCs). EDCs pose a serious health concern since they may cause a wide range of health problems, starting from pre-birth till adult lifelong exposure. We herein provide an overview of the main classes of xenoestrogens, which are classified on the basis of their origin, their structures and their occurrence in the food chain. Furthermore, their either beneficial or toxic effects on human health are discussed in this review.

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“…Estriol stimulated MG-63 cells proliferation via ER and prevented bone loss (Luo & Liao 2003). Equol, a phytoestrogen, promoted the bone formation increasing ALP activity and the MG-63 cells proliferation through ER activation (Wang et al 2014). Phytoestrogens stimulated transcriptional activity of an ERE-luciferase reporter gene via ERb and had osteogenic activity (Tang et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of porcine placenta in a menopausal ovariectomized mouse

et al. 2015

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Menopause is a significant physiological phase that occurs as women's ovaries stop producing ovum and the production of estrogen declines. Human placenta and some amino acids are known to improve menopausal symptoms. In this study, we investigated that porcine placenta extract (PPE) and arginine (Arg), a main amino acid of PPE, would have estrogenic activities in ovariectomized (OVX) mice as a menopause mouse model, human breast cancer cell line (MCF-7) cells, and human osteoblast cell line (MG-63) cells. PPE or Arg significantly inhibited the body weight and increased the vagina weight compared to the OVX mice. PPE or Arg ameliorated the vaginal atrophy in the OVX mice. The levels of 17b-estradiol and the activities of alkaline phosphatase (ALP) were significantly increased by PPE or Arg in the serum of OVX mice. Trabecular bone parameters such as bone mineral density and porosity were also improved by PPE or Arg in the OVX mice. In the MCF-7 and MG-63 cells, PPE or Arg significantly increased the cell proliferation, estrogen receptor b mRNA expression, and estrogen-response elements luciferase activity. Finally, PPE or Arg increased the activations of ALP and extracellular signalregulated kinase 1/2 in the MG-63 cells. These results indicate that PPE or Arg would have estrogenic and osteoblastic activity. Therefore, PPE or Arg may be useful as new pharmacological tools for treating menopausal symptoms including osteoporosis. Free Korean abstract: A Korean translation of this abstract is freely available at

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Equol promotes rat osteoblast proliferation and differentiation through activating estrogen receptor

Cited by 31 publications

References 36 publications

Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Combined Effects of Soy Isoflavones and β-Carotene on Osteoblast Differentiation

Risks and benefits related to alimentary exposure to xenoestrogens

Protective effect of porcine placenta in a menopausal ovariectomized mouse

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