Er-Miao-San, a traditional herbal formula containing Rhizoma Atractylodis and Cortex Phellodendri inhibits inflammatory mediators in LPS-stimulated RAW264.7 macrophages through inhibition of NF-κB pathway and MAPKs activation

Chen, Gang; Li, Kai-Kai; Fung, Chak‐Hei; Liu, Cheuk‐Lun; Wong, Hai Ming; Leung, Ping‐Chung; Ko, Chun‐Hay

doi:10.1016/j.jep.2014.04.042

Cited by 65 publications

(37 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, numerous studies have shown that the NF-κB pathway contributes to transcriptional upregulation of inflammatory factors in the pathogenesis of renal dysfunction (20 –23). Activation of the NF-κB is initiated by the signal-induced degradation of IκB proteins (24), which occurs primarily via activation of a kinase called IκB kinase (IKK) (25).…”

Section: Discussionmentioning

confidence: 99%

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation

Gan

Huang

Jiang

et al. 2018

Braz J Med Biol Res

View full text Add to dashboard Cite

Hypertensive renal damage generally occurs during the middle and late stages of hypertension, which is typically characterized by proteinuria and renal inflammation. Captopril, an angiotensin-converting enzyme (ACE) inhibitor, has been widely used for therapy of arterial hypertension and cardiovascular diseases. However, the protective effects of captopril on hypertension-induced organ damage remain elusive. The present study was designed to explore the renoprotective action of captopril in spontaneously hypertensive rats (SHR). The 6-week-old male SHR and age-matched Wistar-Kyoto rats were randomized into long-term captopril-treated (34 mg/kg) and vehicle-treated groups. The results showed that in SHR there was obvious renal injury characterized by the increased levels of urine albumin, total protein, serum creatinine, blood urea nitrogen, renal inflammation manifested by the increased mRNA and protein expression of inflammatory factors including tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and inducible nitric oxide synthase, and enhanced nuclear factor-κB (NF-κB) activation. Captopril treatment could lower blood pressure, improve renal injury, and suppress renal inflammation and NF-κB activation in SHR rats. In conclusion, captopril ameliorates renal injury and inflammation in SHR possibly via inactivation of NF-κB signaling.

show abstract

Section: Discussionmentioning

confidence: 99%

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation

Gan

Huang

Jiang

et al. 2018

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Of these, IL-1β, IL-6, PGE 2 are regarded as endogenous inflammatory mediators, which is associated with fever. TNF-α plays a key role in inflammation due to reactions by activating T cells and macrophages through upregulating other pro-inflammatory cytokines and endothelial adhesion molecules, which enhance the recruitment of leukocytes to the site of inflammation (Andreakos et al, 2002;Chen et al, 2014a). Overproduction of NO triggers a number of cell functions including production of numerous pro-inflammatory mediators, signal transduction and apoptosis of local cells contributing to the tissue damage (Chen et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

“…TNF-α plays a key role in inflammation due to reactions by activating T cells and macrophages through upregulating other pro-inflammatory cytokines and endothelial adhesion molecules, which enhance the recruitment of leukocytes to the site of inflammation (Andreakos et al, 2002;Chen et al, 2014a). Overproduction of NO triggers a number of cell functions including production of numerous pro-inflammatory mediators, signal transduction and apoptosis of local cells contributing to the tissue damage (Chen et al, 2014a). In this work, RDN dramatically reduced the production of NO, PGE 2 , IL-6 and IL-1β at a dose-dependent manner in LPS-induced macrophages, and the results revealed that RDN possessed the anti-inflammatory effect through inhibiting the production of inflammatory mediators.…”

Section: Discussionmentioning

confidence: 99%

“…Network analysis predicted that RDN could regulate MAPK pathway (ERK1/2, p38 and JNK), and further influence the expression of chemokines and cytokines (Chen et al, 2014a). Likely, MAPKs and NF-κB are well known to two pivotal pathways in the expression of inflammatory mediators (Choi et al, 2012;Corriveau and Danner, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Many natural and synthetic agents, which were reported to inhibit NF-κB activation, target different steps in the NF-κB signal pathway including phosphorylation, ubiquitination, degradation, nuclear translocation and DNA binding (Zhang et al, 2012). p-p65 at serine 536 (Ser 536 ) has been showed to be important in initiating transcription of NF-κB in macrophages, and the phosphorylation on Ser 536 increased the p65 transcription activity (Chen et al, 2014a). In our study, the level of p-p65 (Ser 536 ) significantly increased by the stimulation of LPS in 20 min.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Insight into the molecular mechanism of a herbal injection by integrating network pharmacology and in vitro

Zhang

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

The compatibility of six alkaloids in ermiao pill explored by a comparative pharmacokinetic and network pharmacological study

Xia

et al. 2019

Biomedical Chromatography

View full text Add to dashboard Cite

Ermiao Pill (EMW), a traditional Chines medicine (TCM), composed of a two‐herb pair, Phellodendri Cortex (PC) and Atractylodis Rhizome (AR), is used for the treatment of pelvic inflammatory disease and inflammatory‐related diseases. However, the underlying mechanism is still unknown. Compatibility plays a crucial role in the complex drugs such as those used in traditional Chinese medicine. We propose a compositive strategy, which integrated pharmacokinetics and network pharmacology to explore the compatibility in EMW. Firstly, a simple, rapid, and selective method based on UPLC–MS/MS was established and validated for simultaneous qualification of six alkaloids in rat plasma, which was used for a comparative pharmacokinetic study of EMW and its constituent herb PC. The concentration–time profiles suggested that AR might reduce the toxicity of some alkaloids in EMW. Secondly, network pharmacology analysis showed that the key protein PTGS2 was targeted by four alkaloids, and that the competition among them might be allevited by AR. Thirdly, molecular docking exhibited interactions between the alkaloids and PTGS2 through H and π–π bonds, and the same residue formed interactions with different alkaloids, which account for the toxicity of these alkaloids, and these were confirmed by the cell viability assay. The combination of pharmacokinetics and network pharmacology clarified the compatibility in EMW.

show abstract

Er-Miao-San, a traditional herbal formula containing Rhizoma Atractylodis and Cortex Phellodendri inhibits inflammatory mediators in LPS-stimulated RAW264.7 macrophages through inhibition of NF-κB pathway and MAPKs activation

Cited by 65 publications

References 35 publications

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation

Captopril alleviates hypertension-induced renal damage, inflammation, and NF-κB activation

Insight into the molecular mechanism of a herbal injection by integrating network pharmacology and in vitro

The compatibility of six alkaloids in ermiao pill explored by a comparative pharmacokinetic and network pharmacological study

Contact Info

Product

Resources

About