Eradicating intracellular MRSA via targeted delivery of lysostaphin and vancomycin with mannose-modified exosomes

Yang, Xiaohong; Xie, Beibei; Peng, Haibo; Shi, Gongming; Banne, Sreenivas; Guo, Jingjie; Wang, Chenhui; He, Yun

doi:10.1016/j.jconrel.2020.11.045

Cited by 63 publications

(54 citation statements)

References 42 publications

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“…Another method is gene editing, where genetic modification of parental cells is used to incorporate therapeutic cargo such as RNA and proteins that cannot be directly incorporated into exosomes [11]. Principle of post-isolation exosomes modification methods, where the desired therapeutic agents can be encapsulated in purified exosomes, directly after their isolation from cells, either through passive (co-incubation) or active incorporation methods (summarized from [14][15][16][79][80][81][82][83][84]).…”

Section: Pre-isolation Modification Methodsmentioning

confidence: 99%

“…After the diffusion of the cargo, the membrane integrity of the exosomes is restored [8]. One of the active incorporation methods is electroporation, in which pores are temporarily formed in the phospholipid bilayer of exosomes due to the electric field in a conductive solution, Principle of post-isolation exosomes modification methods, where the desired therapeutic agents can be encapsulated in purified exosomes, directly after their isolation from cells, either through passive (co-incubation) or active incorporation methods (summarized from [14][15][16][79][80][81][82][83][84]).…”

Section: Pre-isolation Modification Methodsmentioning

confidence: 99%

“…Human chorionic gonadotropin was efficiently loaded (40.55% ± 4.21%) into exosomes isolated from uterine fluid using a sonication process by Hajipour et al [80]. While Yang et al [84] achieved 15.52% ± 2.38% encapsulation efficiency by encapsulating the antibacterial drug lysostaphin into mannosylated exosomes by sonication and 22.15% ± 3.21% by encapsulating vancomycin. Extrusion is a method in which a mixture of exosomes and cargo is extruded through a membrane with a pore size between 100 and 400 nm using a lipid extruder.…”

Section: Post-isolation Modification Methodsmentioning

confidence: 99%

“…Figure 5. Principle of post-isolation exosomes modification methods, where the desired therapeutic agents can be encapsulated in purified exosomes, directly after their isolation from cells, either through passive (co-incubation) or active incorporation methods (summarized from[14][15][16][79][80][81][82][83][84]). …”

mentioning

confidence: 99%

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Exosomes Engineering and Their Roles as Therapy Delivery Tools, Therapeutic Targets, and Biomarkers

Kučuk

Primožič

Knez

et al. 2021

IJMS

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Exosomes are becoming increasingly important therapeutic biomaterials for use in a variety of therapeutic applications due to their unique characteristics, especially due to the ineffectiveness and cytotoxicity of some existing therapies and synthetic therapeutic nanocarriers. They are highly promising as carriers of drugs, genes, and other therapeutic agents that can be incorporated into their interior or onto their surface through various modification techniques to improve their targeting abilities. In addition, they are biocompatible, safe, and stable. The review focuses on different types of exosomes and methods of their preparation, including the incorporation of different kinds of cargo, especially for drug delivery purposes. In particular, their importance and effectiveness as delivery vehicles of various therapeutic agents for a variety of therapeutic applications, including different diseases and disorders such as cancer treatment, cardiovascular and neurodegenerative diseases, are emphasized. Administration routes of exosomes into the body are also included. A novelty in the article is the emphasis on global companies that are already successfully developing and testing such therapeutic biomaterials, with a focus on the most influential ones. Moreover, a comparison of the advantages and disadvantages of the various methods of exosome production is summarized for the first time.

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Section: Pre-isolation Modification Methodsmentioning

confidence: 99%

Section: Pre-isolation Modification Methodsmentioning

confidence: 99%

Section: Post-isolation Modification Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Exosomes Engineering and Their Roles as Therapy Delivery Tools, Therapeutic Targets, and Biomarkers

Kučuk

Primožič

Knez

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…EXO have been isolated from other immune cells to tap into their therapeutic potential, including Tregs [ 180 , 181 , 182 ], B lymphoblasts [ 183 , 184 ], natural killer cells [ 185 ], and mast cells [ 186 ]. Notably, EXO isolated from macrophages have been loaded with antibiotics, including linezolid and vancomycin, to inhibit intracellular infection by methicillin-resistant Staphylococcus aureus (MRSA) [ 187 , 188 ]. EXO derived from muscle cells loaded with viral antigens to be used as a vaccine and induce specific cytotoxic T lymphocyte immunity [ 189 ].…”

Section: Exosome-based Therapiesmentioning

confidence: 99%

Selective Antimicrobial Therapies for Periodontitis: Win the “Battle and the War”

Elashiry

Morandini

Timothius

et al. 2021

IJMS

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Traditional antimicrobial therapies for periodontitis (PD) have long focused on non-selective and direct approaches. Professional cleaning of the subgingival biofilm by instrumentation of dental root surfaces, known as scaling and root planning (SRP), is the mainstay of periodontal therapy and is indisputably effective. Non-physical approaches used as adjuncts to SRP, such as chemical and biological agents, will be the focus of this review. In this regard, traditional agents such as oral antiseptics and antibiotics, delivered either locally or systemically, were briefly reviewed as a backdrop. While generally effective in winning the “battle” against PD in the short term, by reducing its signs and symptoms, patients receiving such therapies are more susceptible to recurrence of PD. Moreover, the long-term consequences of such therapies are still in question. In particular, concern about chronic use of systemic antibiotics and their influence on the oral and gut microbiota is warranted, considering antibiotic resistance plasmids, and potential transfer between oral and non-oral microbes. In the interest of winning the “battle and the war”, new more selective and targeted antimicrobials and biologics for PD are being studied. These are principally indirect, blocking pathways involved in bacterial colonization, nutrient acquisition, inflammation or cellular invasion without directly killing the pathogens. This review will focus on current and prospective antimicrobial therapies for PD, emphasizing therapies that act indirectly on the microbiota, with clearly defined cellular and molecular targets.

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Combination Therapy with Resveratrol and Celastrol Using Folic Acid‐Functionalized Exosomes Enhances the Therapeutic Efficacy of Sepsis

Zheng,

Xing,

Sun

et al. 2023

Adv Healthcare Materials

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Overactivated macrophages are a prominent feature of many inflammatory and autoimmune diseases, including sepsis. Attention and regulation of macrophages activity is of great significance for sepsis treatment. Here we showed that folic acid‐functionalized exosomes accumulated in the lung of septic mice and specifically targeted inflammatory macrophages. We therefore designed FA‐functionalized exosomes co‐loaded with resveratrol (an anti‐inflammatory polyphenol) and celastrol (an immunosuppressive pentacyclic triterpenoid; FA‐Exo/R+C), which exhibited powerful anti‐inflammatory and immunosuppressive activities against LPS‐stimulated macrophages in vitro by regulating NF‐κB and ERK1/2 signaling pathways. Encouraged by these positive data, the efficacy of FA‐Exo/R+C was systematically investigated in an LPS‐induced mouse sepsis model. FA‐Exo/R+C showed striking therapeutic benefits in terms of attenuated cytokine storm, reduced acute lung injury, and increased survival of septic mice by inhibiting the inflammation and proliferation of proinflammatory M1 macrophages. Importantly, multiple administrations of FA‐Exo/R+C significantly enhanced and prolonged the protective effect, and resisted re‐challenge to LPS. Collectively, the strategy of co‐delivering drugs combination through functionalized exosomes offers a new avenue for sepsis treatment.This article is protected by copyright. All rights reserved

show abstract

Eradicating intracellular MRSA via targeted delivery of lysostaphin and vancomycin with mannose-modified exosomes

Cited by 63 publications

References 42 publications

Exosomes Engineering and Their Roles as Therapy Delivery Tools, Therapeutic Targets, and Biomarkers

Exosomes Engineering and Their Roles as Therapy Delivery Tools, Therapeutic Targets, and Biomarkers

Selective Antimicrobial Therapies for Periodontitis: Win the “Battle and the War”

Combination Therapy with Resveratrol and Celastrol Using Folic Acid‐Functionalized Exosomes Enhances the Therapeutic Efficacy of Sepsis

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