Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM)

Miyazaki, Junji; Tan, Beng H.; Errington, Steve

doi:10.1007/s11240-010-9741-5

Cited by 32 publications

(17 citation statements)

References 27 publications

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“…PPM is a relatively new, broad-spectrum biocide used in plant tissue culture and can effectively prevent microbial contamination of plant cell and tissue culture without affecting the seed germination and callus regeneration as reported for various plants such as The data are means ±SE (n = 3), and the different letters indicate significant differences at P < 0.05. cauliflower, chrysanthemum, and petunia, etc. (George and Tripepi, 2001;Miyazaki et al, 2010;Niedz, 1998;Rihan et al, 2012). As shown in Fig.…”

Section: Resultsmentioning

confidence: 94%

Effects of Cultivar and Ethanol Disinfection on Aseptic Germination of Loquat (Eriobotrya japonica) Seeds

Dong

Zhao

et al. 2017

horts

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Loquat (Eriobotrya japonica Lindl.) is an economically important subtropical fruit tree, originating and commercially produced mainly in China and Japan. The aseptic seed germination of 13 loquat cultivars, including 9 white-fleshed cultivars and 4 red-fleshed ones, was studied, and the relationship between the germination rate and the content of endogenous ABA in seeds was explored. The germination rate and the seedling height at 80 days after sowing of white-fleshed cultivars were generally higher than that of red-fleshed ones. The ABA content in seeds was generally lower in white-fleshed cultivars, and the ABA content negatively correlated with the germination rate at 80 days after sowing. A moderate detrimental effect of 75% ethanol disinfection on aseptic germination of loquat seeds was observed, especially, for the cultivar Dahongpao, and the germination rate and seedling height were reduced by around half and one-third, respectively. The addition of plant preservative mixture (PPM) to the germination medium at a final concentration of 25 mg·L−1 effectively controlled contamination when the 75% ethanol disinfection procedure was omitted.

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Section: Resultsmentioning

confidence: 94%

Effects of Cultivar and Ethanol Disinfection on Aseptic Germination of Loquat (Eriobotrya japonica) Seeds

Dong

Zhao

et al. 2017

horts

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“…Therefore, only the bacterial isolate found in T7 was identified by molecular analyzes. The presence of endophytic bacteria in apparently decontaminated plant material may be explained by the fact that these contaminants are located in the intercellular spaces, what prevents biocide penetration, making it difficult to eradicate endophytic microorganisms (Miyazaki et al 2010).…”

Section: Resultsmentioning

confidence: 99%

In vitro establishment of Bambusa oldhamii Munro from field-grown matrices and molecular identification of endophytic bactéria

Pasqualini

Schneider

Fraga

et al. 2019

Pesqui. Agropecu. Trop.

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In plant micropropagation, the establishment stage is difficult, due to the presence of microorganisms in tissues from field-grown matrices, especially for bamboo. This study aimed to establish an efficient asepsis protocol for Bambusa oldhamii explants from field plants, as well as to carry out the molecular identification of a possible endophytic bacterial isolate. The explants were exposed to 70 % alcohol, 1 % sodium hypochlorite (NaOCl), 0.1 % mercuric chloride (HgCl2), thiophanate-methyl (Cercobin®) and chlorhexidine digluconate (2 % Riohex®) in different combinations, and introduced into Murashige and Skoog culture medium (solid or liquid), supplemented or not with 4 mL L-1 of Plant Preservative Mixture (PPMTM), totaling seven treatments. The asepsis and immersion of the explants in the liquid culture medium containing 4 mL L-1 of PPMTM visually inhibited the bacterial and fungal growth, allowed the development of shoots with a mean length of 2.2 cm and posterior subcultures, being the best treatment used for the in vitro establishment of B. oldhamii. The molecular identification of an endophytic bacterium performed by 16S rDNA sequencing allowed to identify the bacterial isolate as Ralstonia sp., with 100 % of similarity, and the phylogenetic analysis grouped it with Ralstonia pickettii. In addition, the bacterial isolate showed to be sensitive to 4 mL L-1 of PPMTM by the minimum inhibitory concentration test.

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“…Plants in culture were maintained in a temperature-controlled room at 23 • C with a 16 h light/8 h dark photoperiod using 36-watt white fluorescent tubes. Plants were grown in half-strength Murashige and Skoog medium (Sigma, St Louis, MO USA) with the addition of 3% (w/v) sucrose and 1% (v/v) plant preservative mixture (PPM) (Plant Cell Technology, Washington, DC, USA) to inhibit fungal contamination [35]. Plants were re-flasked every 3 months in this medium.…”

Section: Plant Materialsmentioning

confidence: 99%

The Induction and Roles Played by Phi Thickenings in Orchid Roots

Idris

Collings

2019

Plants

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Phi thickenings are specialised secondary wall thickenings present in the root cortex of many plant species, including both angiosperms and gymnosperms. While environmental stresses induce phi thickenings, their role(s) in the root remain unclear. Suggested functions include regulation of transport through the apoplast in a manner similar to the Casparian strip, limiting fungal infections, and providing mechanical support to the root. We investigated phi thickening induction and function in Miltoniopsis sp., an epiphytic orchid. As movement of a fluorescent tracer through the apoplast was not blocked by phi thickenings, and as phi thickenings developed in the roots of sterile cultures in the absence of fungus and did not prevent fungal colonisation of cortical cells, the phi thickenings in Miltoniopsis did not function as a barrier. Phi thickenings, absent in roots grown on agar, remained absent when plants were transplanted to moist soil, but were induced when plants were transplanted to well-drained media, and by the application of water stress. We suggest that it is likely that phi thickenings stabilise to the root during water stress. Nevertheless, the varied phi thickening induction responses present in different plant species suggest that the phi thickenings may play multiple adaptive roles depending on species.

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Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM)

Cited by 32 publications

References 27 publications

Effects of Cultivar and Ethanol Disinfection on Aseptic Germination of Loquat (Eriobotrya japonica) Seeds

Effects of Cultivar and Ethanol Disinfection on Aseptic Germination of Loquat (Eriobotrya japonica) Seeds

In vitro establishment of Bambusa oldhamii Munro from field-grown matrices and molecular identification of endophytic bactéria

The Induction and Roles Played by Phi Thickenings in Orchid Roots

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