Eradication of PPV and PNRSV Viruses from Three Peach Cultivars Using Thermotherapy In Vitro, Including Optimization of Microshoots’ Multiplication and Rooting Medium

Abstract

Peach cultivars (’Elberta’, ‘Red Top’, and ’Dixie Red’) were studied for their in vitro adoptability and performance in producing virus-free plantlets. The thermotherapy method with increasing temperatures (25 °C to 37 °C) was applied for the elimination of famous peach infecting plum pox viruses (PPVs) and prunus necrotic ringspot virus (PNRSV), and the DS-ELISA test and RT-PCR technique were performed to confirm the production of virus-free microshoots. The application of 30 mg L−1 of Fe-EDDHA treatment resulted in the best performance for culture establishment of all cultivars, where the best subsequent morphological performance in terms of branch and leaf numbers was recorded for the ’Dixie Red’ cultivar in MS medium, supplemented with 0.5 mgL−1 of gibberellic acid (GA3) and 0.5 mg L−1 of 6-Benzylaminopurine (BAP). At the regeneration stage, the highest (26.96 mm) and lowest (18.43 mm) shoot lengths were obtained from the ’Dixie Red’ cultivar treated with GA3 (2 mg L−1) + thidiazuron (TDZ) (2.5 mg L−1) and the ’Red Top’ cultivar treated with GA3 (1 mg L−1) + TDZ (1 mg L−1), respectively. The leaf numbers were affected by the application of growth regulators, where the ’Elberta’ cultivar under GA3 (2 mg L−1) + TDZ (2.5 mg L−1) treatment showed the highest numbers and the ’Red Top’ cultivar under GA3 (1 mg L−1) + TDZ (1 mg L−1) showed the lowest mean values. The thermotherapy treatment and micropropagation of shoot tips resulted in 100% virus-free plantlets, as confirmed by both applied diagnostic methods. The result of the application of the rooting stage with growth regulators on ’Elberta’ plantlets showed the best performance (90%) in ½ MS medium supplemented with 0.5–1 mg L−1 of IBA, which was significantly higher than the same treatment in MS medium. The obtained results should constitute the basis for further optimization of the multiplication and rooting of virus-free peach plantlets to be served for nurseries and planation orchards.

Elimination of cherry virus A from Prunus domestica ‘Besztercei Bt. 2’ using in vitro techniques

“…Abdullahi and Lawrence (2022) reported that 7 days of heat treatment at 37/32°C prior to meristem preparation was sufficient to eliminate apple chlorotic leaf spot virus (ACLSV) from Shiro plum, while Koubouris et al (2007) applied heat treatment for 20 days at 35-37 °C to eliminate PPV from the apricot cultivar 'Bebecou'. Hesari et al (2022) applied heat treatment at 37 °C for 4 weeks and this resulted in 100% PPV-free and Prunus necrotic ring spot virus (PNRSV)-free peach plants. A 45-day-long period at 37/35°C was suitable for eliminating PPV, PNRSV and prune dwarf virus (PDV) from apricot cultivars (Křižan and Ondrušiková 2009).…”