Eradication of PVS from potato clones through excision of meristems from in vitro, heat-treated shoot tips

Brown, Charles R.; Kwiatkowski, Stephen; Martin, M. W.; Thomas, P. E.

doi:10.1007/bf02854831

Cited by 17 publications

(7 citation statements)

References 9 publications

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“…Meristem-tip culture has been used widely for the production of virus-free plant material in many species propagated mainly or exclusively by vegetative means (Kartha and Gamborg 1975;Brown et al 1988;Ayabe and Sumi 2001), but in woody species and particularly in peaches the available reports are rather limited (Navarro 1988;Shu and Timon 1993). Given that there are no available antivirus remedies, thermotherapy combined with meristem-tip culture is the main way of producing virus-free plants (Walkey 1980), since sources of virus resistance among peach and nectarine cultivars are rather limited (Escalettes et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine

et al. 2003

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The plum pox virus (PPV) and prunus necrotic ringspot virus (PNRSV) cause serious disease problems in stone-fruit trees. In this work, the possibility of obtaining plant material free from these viruses through thermotherapy and meristem-tip culture from infected nectarine shoots (Prunus persica var. nectarina Max, cv. 'Arm King') was studied. In addition, the detection of these viruses in in vitro cultures and young acclimatized plantlets with double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was studied. Meristem-tip explants (0.8-1.3 mm) derived from sprouted buds of winter wood and spring shoots from field grown plants had a 2-5% regeneration response. However, application of thermotherapy to potted nectarine trees (3 weeks at a maximum temperature of 35 degrees C) facilitated excision of longer meristem tips (1.3-2.0 mm) that resulted in a significantly higher regeneration response (38%) in woody plant medium (WPM) without plant growth regulators. Such explants formed multiple shoots with the addition of 8 microM benzylaminopurine and 0.8 microM indoleacetic acid. When they were tested for the presence of PPV and PNRSV, 86% and 81% were found to be virus-free as detected by DAS-ELISA and multiplex RT-PCR, respectively. Individual shoots excised from virus-free cultures readily rooted in vitro (half-strength WPM plus 2 microM indolebutyric acid) and grew to plantlets. The combination of an efficient protocol for virus elimination and the establishment of highly sensitive diagnostics resulted in the production of nectarine plants free from PPV and PNRSV.

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Section: Introductionmentioning

confidence: 99%

Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine

et al. 2003

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“…Healthy germplasm with the ability of storing long term is the fundamental for effective conservation and utilization APGRs (Brown et al 1988, Engelmann 1997, Hasan and Tagaki 1995. Infected propagules deteriorate quickly in storage conditions; therefore it is important to store only healthy samples in the genebanks.…”

Section: Pathogen Free Plants Productionmentioning

confidence: 99%

“…Thermotherapy and meristem-tip culture are most widely used for production of virus free collections in the in-vitro genebank at the International Center for Tropical Agriculture (CIAT) (IPGRI/CIAT 1994). Other successful elimination of viruses from important crop species are cassava mosaic virus from cassava (Kartha and Gamborg 1975), exocortis viroid from Citrus species (Greno et al 1990), potato virus S from potato (Brown et al 1988) and sweet potato yellow dwarf virus from sweet potato plants (Green and Lo 1989).…”

Section: Pathogen Free Plants Productionmentioning

confidence: 99%

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

Joshi

2017

J. Nep. Agric. Res. Counc.

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Agricultural biodiversity is the basis of human life and food security. Nepal with 577 cultivated species possesses huge diversity at varietal as well as landrace levels. In most agricultural crops the rapid genetic erosion due to several reasons is a common phenomenon. Thus, considering the importance of agricultural biodiversity declared by Convention on Biological Diversity for sustainable food production, National Agriculture Genetic Resources Center (NAGRC) has been established for conservation and sustainable utilization of agricultural biodiversity. This paper thus delineates the application of biotechnological tools adopted by NAGRC for effective and efficient conservation and use of agricultural plant genetic resources (APGRs). Among the adopted technologies, tissue bank using shoot tip culture of vegetatively propagating and recalcitrant crops eg potato, sugarcane, banana, sweet potato, etc are in function. Under the molecular marker technology, currently random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers have been used for developing DNA profiles, identifying duplicates in the collections, assessing genetic diversity and screening accessions against economic traits. DNA bank has also been created for storing DNA of indigenous crops and these DNA can be accessed for research and study. Genotypic database has been developed for chayote, finger millet, wheat and maize for identification and selection of the accessions.

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“…Various Agrobacterium-mediated transformations have produced potato lines expressing genes that confer pathogen-derived resistance to viruses. Transgenic lines have been developed that are highly resistant, but not immune, to infection by PLRV, PVY, and PVX [92] . While aphids can still acquire virus from low titre plants, efficiency of transmission is greatly reduced [64] .…”

Section: Genetic Controlmentioning

confidence: 99%

Innovative Approaches towards Aphid Resistance Prevention in Brassica Crops

Jain¹,

Tiwari²

2017

Int. J. Life. Sci. Scienti. Res.

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ABSTRACT-Brassica juncea, the Indian mustard supplies a big amount of edible oil demand in India. Biotic and Abiotic factors were responsible for serious reductions in B. juncea production in India. Several control measurements had been taken to prevent the losses in crop. Biochemical control, Biological control and Genetic control are some of the preventive methods used in this study to evaluate the yield loss in Indian Mustard. In India around 39 million hectares of land was under mustard cultivation with a production of 10 million tonnes. It was estimated that the demand for oilseed in India will be around 34 million tonnes by the year 2020. 41% of this demand (14 million tonnes) had to be met by mustard alone. Apart from its use as oil, it has got some medicinal properties too. Among the oilseed crops grown in India, mustard (Brassica sp.) was one the most important ones. In India around 26.11 million hectares of land was under mustard cultivation during the year 2009-2010 (4 th advanced estimates released on 19.07.2010 by Ministry of Agriculture, Govt. of India). It was estimated that the demand for oilseed in India will be around 34 million tonnes by the year 2020. 41% of this demand 14 million tonnes must be met by mustard alone. The production of Indian mustard was severely affected by mustard aphids. In this review, we had studied some of the control method to avoid aphid infestation, which will severely affect the crop production.

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Eradication of PVS from potato clones through excision of meristems from in vitro, heat-treated shoot tips

Cited by 17 publications

References 9 publications

Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine

Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

Innovative Approaches towards Aphid Resistance Prevention in Brassica Crops

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