Erasure of CpG methylation in
            <i>Arabidopsis</i>
            alters patterns of histone H3 methylation in heterochromatin

Tariq, Muhammad; Saze, Hidetoshi; Probst, Aline V.; Lichota, Jacek; Habu, Yoshiki; Paszkowski, Jerzy

doi:10.1073/pnas.1432939100

Cited by 279 publications

(237 citation statements)

References 33 publications

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“…The cDNA clones of HL2b, Y2b, and CAT3-FLAG were inserted into the cloning site in pBE2113 (Mitsuhara et al, 1996), and the control GFP gene was also cloned in pE2113. These protoplasts were fixed with 2% paraformaldehyde on L-poly-Lys-coated slides (Tariq et al, 2003;Yoo et al, 2007) 24 h after transfection. After rehydration in phosphate-buffered saline (PBS), the fixed protoplasts on the slides were blocked in 2% bovine serum albumin in PBS (30 min, room temperature) and incubated for 1 h at room temperature in 0.5% bovine serum albumin in PBS containing primary antibodies.…”

Section: Immunostaining By In Situ Plamentioning

confidence: 99%

Virus-Induced Necrosis Is a Consequence of Direct Protein-Protein Interaction between a Viral RNA-Silencing Suppressor and a Host Catalase

et al. 2011

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Many plant host factors are known to interact with viral proteins during pathogenesis, but how a plant virus induces a specific disease symptom still needs further research. A lily strain of Cucumber mosaic virus (CMV-HL) can induce discrete necrotic spots on infected Arabidopsis (Arabidopsis thaliana) plants; other CMV strains can induce similar spots, but they are not as distinct as those induced by CMV-HL. The CMV 2b protein (2b), a known RNA-silencing suppressor, is involved in viral movement and symptom induction. Using in situ proximity ligation assay immunostaining and the protoplast assays, we report here that CMV 2b interacts directly with Catalase3 (CAT3) in infected tissues, a key enzyme in the breakdown of toxic hydrogen peroxide. Interestingly, CAT3, normally localized in the cytoplasm (glyoxysome), was recruited to the nucleus by an interaction between 2b and CAT3. Although overexpression of CAT3 in transgenic plants decreased the accumulation of CMV and delayed viral symptom development to some extent, 2b seems to neutralize the cellular catalase contributing to the host defense response, thus favoring viral infection. Our results thus provide evidence that, in addition to altering the type of symptom by disturbing microRNA pathways, 2b can directly bind to a host factor that is important in scavenging cellular hydrogen peroxide and thus interfere specifically with that host factor, leading to the induction of a specific necrosis.Cucumber mosaic virus (CMV) belongs to the genus Cucumovirus and contains tripartite, single-stranded sense RNAs designated RNA1 to RNA3 in decreasing order of molecular mass. RNA1 and RNA2 encode replicase proteins 1a and 2a, respectively, and RNA3 encodes the movement protein 3a. A subgenomic RNA,

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Section: Immunostaining By In Situ Plamentioning

confidence: 99%

Virus-Induced Necrosis Is a Consequence of Direct Protein-Protein Interaction between a Viral RNA-Silencing Suppressor and a Host Catalase

et al. 2011

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“…has been observed in diverse biological systems (Tamaru and Selker, 2001;Fahrner et al, 2002;Jackson et al, 2002;Fuks et al, 2003;Lehnertz et al, 2003;Tariq et al, 2003;Freitag et al, 2004). Therefore, the different H3-m 3 K9 states in the two species prompted us to investigate DNA methylation states in sperm-derived chromatins.…”

Section: Appearance Of H3-k9 Methylation In Pig But Not In Mouse Spmentioning

confidence: 99%

Gradual development of a genome‐wide H3‐K9 trimethylation pattern in paternally derived pig pronucleus

Jeong

Yeo

Park

et al. 2007

Developmental Dynamics

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The cytoplasm of a mature oocyte contains many protein complexes that are programmed to restructure incoming sperm chromatins on fertilization. Of the complicated biochemical events that these functional machineries control, the most impressive and important is epigenetic reprogramming. Despite its importance in epigenetic resetting, or "de-differentiation," of gamete genomes back to an incipient status, the mechanisms of epigenetic reprogramming do not seem to be conserved among mammals. Here, we report that, unlike in the mouse, the pig sperm-derived pronucleus is markedly trimethylated at lysine 9 of histone H3 (H3-m 3 K9), which might be associated with preservation of paternally derived cytosine methylation in pig zygotes. The male H3-m 3 K9 pattern is gradually established during pronucleus development, and this process occurs independently of DNA replication. Considering these unique epigenetic features, the pig zygote is, we believe, suited to serve as another model of epigenetic reprogramming that is antithetical to the well-characterized mouse model. Developmental Dynamics 236:1509 -1516, 2007.

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“…The METs maintain CpG methylation (Cao and Jacobsen, 2002b;Tariq et al, 2003), CMTs maintain non-CpG methylation (Cao and Jacobsen, 2002b), and DRMs initiate de novo cytosine methylation at CpG, CpNpG, and asymmetric sites (Cao and Jacobsen, 2002a). DRMs also play a role in maintenance of methylation at CpNpG and asymmetric sites (Cao and Jacobsen, 2002b).…”

Section: Evidence For Epigenetic Modifications In Habituated Callus Cmentioning

confidence: 99%

A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture

Pischke¹,

Huttlin²,

Hegeman³

et al. 2006

Plant Physiology

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For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated calli, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of shoot structures are all cytokinin-dependent processes. Habituation refers to a naturally occurring phenomenon whereby callus cultures, upon continued passage, lose their requirement for cytokinin. Earlier studies of calli with a higher-than-normal cytokinin content indicate that overproduction of cytokinin by the culture tissues is a possible explanation for this acquired cytokinin independence. A transcriptome-based analysis of a well established habituated Arabidopsis (Arabidopsis thaliana) cell culture line was undertaken, to explore genome-wide expression changes underlying the phenomenon of habituation. Increased levels of expression of the cytokinin receptor CRE1, as well as altered levels of expression of several other genes involved in cytokinin signaling, indicated that naturally acquired deregulation of cytokinin-signaling components could play a previously unrecognized role in habituation. Up-regulation of several cytokinin oxidases, downregulation of several known cytokinin-inducible genes, and a lack of regulation of the cytokinin synthases indicated that increases in hormone concentration may not be required for habituation. In addition, up-regulation of the homeodomain transcription factor FWA, transposon-related elements, and several DNA-and chromatin-modifying enzymes indicated that epigenetic changes contribute to the acquisition of cytokinin habituation.

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Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin

Cited by 279 publications

References 33 publications

Virus-Induced Necrosis Is a Consequence of Direct Protein-Protein Interaction between a Viral RNA-Silencing Suppressor and a Host Catalase

Virus-Induced Necrosis Is a Consequence of Direct Protein-Protein Interaction between a Viral RNA-Silencing Suppressor and a Host Catalase

Gradual development of a genome‐wide H3‐K9 trimethylation pattern in paternally derived pig pronucleus

A Transcriptome-Based Characterization of Habituation in Plant Tissue Culture

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