Erdosteine enhances mucociliary clearance in rats with and without airway inflammation

Hosoe, Hisashi; Kaise, Toshihiko; Ohmori, Kenji

doi:10.1016/s1056-8719(98)00053-7

Cited by 14 publications

(8 citation statements)

References 28 publications

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“…Mycoplasma hyopneumoniae, Aujeszky disease virus, porcine reproductive and respiratory syndrome virus with Bordetella bronchiseptica and crude Actinobacillus pleuropneumoniae cytotoxin are very effective agents to promote PmAinduced pneumonia [5,8,9,22]. In our study, E. coli endotoxins, which have already been shown to inhibit mucociliary clearance in rats and to induce a transient acute lung inflammation in pigs which remains in the lung for a maximum of 48 h, were intratracheally injected to promote PmA infection [20,27]. The lung injuries observed in the present study, especially subacute exudative bronchopneumonia involving migration of neutrophils in alveoli and bronchi, marked alveolar macrophage proliferation, and peribronchiolar and alveolar lymphocytic infiltration were in full agreement with studies devoted to Pasteurella multocida infection [9,15].…”

Section: Discussionmentioning

confidence: 88%

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

et al. 2004

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-The aims of this study were (1) to correlate cough and body temperature (BT) with the severity of bronchopneumonia in pigs, (2) to determine whether these clinical signs can be used to early diagnose bronchopneumonia and (3) to assess the predictive values of cough and BT regarding lung lesions. Bronchopneumonia was induced by administering E. coli endotoxin (LPS) combined with Pasteurella multocida type A (PmA) in the trachea of 13 piglets. Saline-instilled negative controls (n = 8), PmA inoculated (n = 6) and LPS instilled (n = 5) groups were also constituted. Cough and BT were recorded daily while the bronchopneumonia severity was assessed using bronchoalveolar lavage fluid (BALF) cytology, cytokines and measurement of lung lesion volume. Changes in expiratory breathing pattern were also measured (Penh). The combination of LPS and PmA induced a subacute bronchopneumonia characterised by macrophage, neutrophil, and lymphocyte infiltration, changes in Penh and an increase in the mRNA level of IFN-γ while IL8, IL-18 and TNF-α mRNA levels remained unchanged. The daily body weight gain of infected animals was significantly reduced. Cough and BT changes were proportional to the intensity of the lung inflammatory process, functional respiratory changes and to the extent of macroscopic lesions. When comparing the individual values of cough and BT to thresholds defined for both parameters, an early diagnosis of pneumonia was possible. Considering the pooled data of each group, it was possible to define thresholds allowing an early segregation between the groups of diseased and healthy piglets. The daily values of cough and BT were predictive for the volume of lung lesions recorded at the end of the trial. In conclusion, cough and BT appear as potential indicators for the intensity and the evolution of the respiratory disease. They also seem to be good predictors for the magnitude of lung lesions and weight gain recorded at the study endpoint.

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Section: Discussionmentioning

confidence: 88%

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

et al. 2004

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“…On the other hand, no significant changes were observed in SP-B and SP-C expression following AH treatment in our previous study 9 . Therefore, the suppressive effects of AH on lung inflammation might be exerted via mechanisms other than increasing SPs, such as effects on excretion and other processes 27 , 28 . In fact, pleural thickening attributable to foreign body granuloma with infused TISMO tended to be decreased in Group 3 compared with Group 2 in this study, indicating that AH had the potential to excrete biological foreign bodies.…”

Section: Discussionmentioning

confidence: 99%

Effects of the expectorant drug ambroxol hydrochloride on chemically induced lung inflammatory and neoplastic lesions in rodents

et al. 2018

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Ambroxol hydrochloride (AH) is an expectorant drug used to stimulate pulmonary surfactant and serous airway secretion. Surfactant proteins (SPs) are essential for maintaining respiratory structure and function, although SP expression has also been reported in lung inflammatory and proliferative lesions. To determine whether AH exerts modulatory effects on these lung lesions, we examined its effects on pleural thickening induced by intrathoracic administration of dipotassium titanate (TISMO) in A/JJmsSlc (A/J) mice. We also analyzed the modulatory effects of AH on neoplastic lung lesions induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in A/J mice and by N-nitrosobis (2-hydroxypropyl) amine (DHPN) in F344/DuCrlCrj (F344) rats. A/J mice treated with TISMO showed decreased body weight, increased white blood cell (WBC) counts, and pleural thickening caused by pleuritis and poor general condition. However, A/J mice treated with TISMO + 120 ppm showed significant recovery of body weight and WBC counts to the same levels as those of A/J mice not treated with TISMO, although no significant differences were observed in histopathological changes including the immunohistopathological expression of IL-1β in the lung and maximum pleural thickness regardless of AH treatment. In the NNK and DHPN experiments, no significant differences in body weight, hematology, plasma biochemistry, and histopathological changes were associated with AH concentration. These results suggest that AH potentially exerts anti-inflammatory effects but does not have a direct suppressive effect on lung tumorigenesis in rodents.

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“…In addition, 30 and 100 μM NAC decreased both elasticity and viscosity of the secretions from these cells at both 8 and 24 h of incubation (Seagrave et al, 2012). Erdosteine increased mucociliary clearance at doses above 600 mg/kg in normal rats and at 300 mg/kg in LPS-treated rats (Hosoe et al, 1998). Thus, the concentrations used here are intermediate.…”

Section: Discussionmentioning

confidence: 99%

“…In our system, we observe no effects of the mucolytics after 60 min incubation. Reported effects of NAC and erdosteine were observed at three (Seagrave et al, 2012) and two h (Hosoe et al, 1998), respectively. Our incubation time may be too short but we cannot increase incubation times beyond 60 min due to loss of tissue viability.…”

Section: Discussionmentioning

confidence: 99%

Hyper-osmolarity and calcium chelation: Effects on cystic fibrosis mucus

Ermund

Meiss

Gustafsson

et al. 2015

European Journal of Pharmacology

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A non-functional Cystic Fibrosis Transmembrane conductance Regulator (CFTR) leads to the disease cystic fibrosis (CF). Although the CFTR is expressed in multiple organs, pulmonary disease is the major cause of illness and death in patients with CF. Stagnant mucus, causing airway obstruction, bacterial overgrowth, persistent inflammation and tissue destruction characterizes the disease, but how the defect in CFTR function is coupled to the mucus phenotype is still controversial. We have recently shown that bicarbonate ions passing through CFTR are necessary for proper unfolding of the MUC2 mucin, thus highlighting the importance of bicarbonate ion transport via the CFTR and the ability of these ions to raise the pH and chelate calcium bound to the mucin as the important steps in forming normal mucus. In order to find potential CF treatments and expand our knowledge about the usefulness of bicarbonate as an active ingredient in formulations to alleviate mucus plugging, we used an Ussing-type chamber and explants from the F508del-CFTR mutant mouse ileum to test the effect of calcium chelators on mucus attachment, either in isolation or in combination with osmolytes such as mannitol or hypertonic saline. We found that increasing the concentration of bicarbonate, both alone or in combination with increased osmolarity of the solution, detached the otherwise attached CF mucus.

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Erdosteine enhances mucociliary clearance in rats with and without airway inflammation

Cited by 14 publications

References 28 publications

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

Effects of the expectorant drug ambroxol hydrochloride on chemically induced lung inflammatory and neoplastic lesions in rodents

Hyper-osmolarity and calcium chelation: Effects on cystic fibrosis mucus

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