Erectogenic and Aphrodisiac Property ofMoringa oleifera: Involvement of Soluble Epoxide Hydrolase Enzyme

Abstract

Soluble epoxide hydrolase (sEH) inhibitors have been reported to improve penile erection; therefore, sEH could be useful for management of erectile dysfunction. Methanolic and aqueous extracts of 30 Indian medicinal plants were screened for their sEH inhibition potential. Fifteen extracts showed >50% inhibition when screened at 50 µg/mL in sEH inhibition assay. Methanolic extract of Moringa oleifera Lam. (Moringaceae) seeds (MEMO) was most potent with IC50 1.7 ± 0.1 µg/mL and was selected for in vitro studies on isolated rat corpus cavernosum smooth muscle and in vivo sexual behaviour studies on healthy and diabetic rats. Rats were divided into five groups, each containing six animals and treated orally with either water, vehicle (1% Tween-20), MEMO (45 and 90 mg/kg/day for 21 days), and standard drug, sildenafil (5 mg/kg/day for 7 days). An equal number of female rats were used, and the effect of MEMO and sildenafil was compared with that of vehicle. MEMO significantly relaxed isolated rat corpus cavernosum smooth muscle at 0.1-100 µg/mL in vitro and significantly increased (p < 0.05) sexual activity, intracavernous pressure/mean arterial pressure in normal and diabetic rats. The increase in erectile function of rats by MEMO could be because of its sEH inhibitory activity. Copyright © 2016 John Wiley & Sons, Ltd.

Modulatory effects of moringa (Moringa oleiferaL.) leaves infested with African mistletoe (Tapinanthus bangwensisL.) on the antioxidant, antidiabetic, and neurochemical indices in high sucrose diet‐induced diabetic‐like phenotype in fruit flies (Drosophila melanogasterM.)

“…This result is in agreement with the report of Pasco and Leopold (2012) where high sugar diet was reported to induce the observable features of type‐2 diabetes in flies. These effects were mitigated by the varieties of moringa leaf in the diet, this might be due to the ability of moringa to inhibit soluble epoxide hydrolase and inhibition of soluble epoxide hydrolase has been reported to ameliorate diabetes and its complications (Goswami et al., 2016; Nathani, Rema, Bruce, & Sumanta, 2018).…”

Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

“…Another study also demonstrated that oral administration of aqueous extract of MO leaves (100, 200 and 500 mg/kg)to male rats for 21 days enhanced male sexual behaviour and libido activity (Zade et al., 2013), an indication of the function of testosterone in the male reproductive system. Also, administration of methanolic extract of MO seeds (45 and 90 mg kg ‐1 day ‐1 ) for 21 days to normal and diabetic male rats enhanced sexual activities (Goswami et al., 2016). Oral administration of ethanol extract of MO leaves (100, 200 and 400 mg/kg) for 30 days significantly increased LH, FSH and testosterone levels (Dafaalla et al., 2017).…”

Seed extracts from Myristica fragrans (Nutmeg) and Moringa oleifera (Drumstick tree) inhibits enzymes relevant to erectile dysfunction and metal-induced oxidative damage in rats' penile tissues

“…It is a medicinal plant with several pharmacological activities which include antidiabetic, antihypertensive, antioxidant, and hypolipidemic activities (Chumark et al ; Verma, Vijayakumar, Mathela, & Rao, ). Extract of drumstick seed and leaves were also reported to exhibit aphrodisiac properties via suppression of soluble epoxide hydrolase (Goswami et al, ) oxidative stress, monoamine oxidase, and phosphodiesterase activities as well as increase in serum testosterone levels (Prabsattroo et al, ). Previous reports have shown that a Drumstick leaf extract inhibited arginase and ACE activities while Nutmeg and Drumstick seeds have been reported to possess aphrodisiac properties (Muchtaridi et al, ; Oboh, Ademiluyi, et al, ; Prabsattroo et al, ).…”