Erg, an Ets-FamiEly member, differentially regulates human collagenase1 (MMP1) and stromelysin1 (MMP3) gene expression by physically interacting with the Fos/Jun complex

Butticè, Giovanna; Duterque-Coquillaud, Martine; Basuyaux, J.P.; Carrère, Séverine; Kurkinen, Markku; Stéhelin, D

doi:10.1016/s0945-053x(97)90078-0

Cited by 86 publications

(112 citation statements)

References 0 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…Although MMP-1 and MMP-3 share homologous gene sequences and similar promoter elements, they do show differential expression in response to several stimuli, probably by recruiting different sets of transcription Ž . factors Buttice et al, 1996 .…”

Section: Discussionmentioning

confidence: 99%

Multiple changes in gene expression in chronic human Achilles tendinopathy

et al. 2001

View full text Add to dashboard Cite

TMŽ . Atlas cDNA cell interaction arrays CLONTECH were used to examine degenerate tissue from four patients with Achilles tendon disorders, in order to identify changes in expression of genes important in cell᎐cell and cell᎐matrix Ž . interactions. The greatest difference between normal post-mortem and degenerate tissue samples was in the level of MMP-3 Ž . stromelysin mRNA, which was down-regulated in all the degenerate samples. Quantitative RT-PCR assay of RNA extracted from paired 'normal' and degenerate Achilles tendon tissue samples taken from tendons during surgery mirrored the results of the arrays. Levels of MMP-3 mRNA were lower, whereas levels of type-I and type-III collagen mRNAs were significantly higher, in the degenerate compared to the 'normal' samples. Immunoblotting of proteins extracted from the same tendon samples showed that three of four normal tissue samples taken from individuals without apparent tendon disorder had much higher levels of MMP-3 protein than 'normal' or degenerate samples from patients with tendinosis. We suggest that MMP-3 may play an important role in the regulation of tendon extracellular matrix degradation and tissue remodelling. ᮊ

show abstract

Section: Discussionmentioning

confidence: 99%

Multiple changes in gene expression in chronic human Achilles tendinopathy

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Finally, the spi-1/Pu-1 and¯i-1 genes are overexpressed as a consequence of retroviral insertion in erythroleukemia (Moreau-Gachelin et al, 1989;BenDavid et al, 1991). The transcription factors of the ets gene family have been the subject of extensive investigation because of their ability to regulate various kinds of genes, including immune response genes (Pongubala et al,1992;Rivera et al, 1993), other oncogenes or genes encoding matrix-degrading proteases (Rorth et al, 1990;Wasylyk et al, 1991;ButticeÂ et al, 1996). Most of the Ets transcriptional factors have been described as transactivators except Erf (ETS2 Repressor factor) and Net which exhibit transcriptional repression properties (Sgouras et al, 1995;Maira et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Most of the Ets transcriptional factors have been described as transactivators except Erf (ETS2 Repressor factor) and Net which exhibit transcriptional repression properties (Sgouras et al, 1995;Maira et al, 1996). Interestingly, some members of the Ets protein family appear to regulate positively or negatively other transcription factor activities (ButticeÂ et al, 1996). Indeed, it has been shown that transcriptional activities of Ets family members require protein-protein interaction, and for example the Elk and Sap proteins interact with p67Srf (Hipskind et al, 1991;Dalton and Treisman, 1992;Price et al, 1995), whereas the Ets-1, Ets-2, Erg, Fli-1 and Pu-1 proteins interact with AP-1 complex (Bassuk and Leiden, 1995;ButticeÂ et al, 1996, Basuyaux et al, 1997, CarreÁ re et al, 1998.…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, some members of the Ets protein family appear to regulate positively or negatively other transcription factor activities (ButticeÂ et al, 1996). Indeed, it has been shown that transcriptional activities of Ets family members require protein-protein interaction, and for example the Elk and Sap proteins interact with p67Srf (Hipskind et al, 1991;Dalton and Treisman, 1992;Price et al, 1995), whereas the Ets-1, Ets-2, Erg, Fli-1 and Pu-1 proteins interact with AP-1 complex (Bassuk and Leiden, 1995;ButticeÂ et al, 1996, Basuyaux et al, 1997, CarreÁ re et al, 1998. Thus, the Ets proteins are important factors in the network of protein-protein interactions that govern transcriptional regulation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular phylogeny of the ETS gene family

et al. 1999

View full text Add to dashboard Cite

We have constructed a molecular phylogeny of the ETS gene family. By distance and parsimony analysis of the ETS conserved domains we show that the family containing so far 29 di erent genes in vertebrates can be divided into 13 groups of genes namely ETS, ER71, GABP, PEA3, ERG, ERF, ELK, DETS4, ELF, ESE, TEL, YAN, SPI. Since the three dimensional structure of the ETS domain has revealed a similarity with the winged-helix ± turn ± helix proteins, we used two of them (CAP and HSF) to root the tree. This allowed us to show that the family can be divided into ®ve subfamilies: ETS, DETS4, ELF, TEL and SPI. The ETS subfamily comprises the ETS, ER71, GABP, PEA3, ERG, ERF and the ELK groups which appear more related to each other than to any other ETS family members. The fact that some members of these subfamilies were identi®ed in early metazoans such as diploblasts and sponges suggests that the diversi®cation of ETS family genes predates the diversi®cation of metazoans. By the combined analysis of both the ETS and the PNT domains, which are conserved in some members of the family, we showed that the GABP group, and not the ERG group, is the one most closely related to the ETS group. We also observed that the speed of accumulation of mutations in the various genes of the family is highly variable. Noticeably, paralogous members of the ELK group exhibit strikingly di erent evolutionary speed suggesting that the evolutionary pressure they support is very di erent.

show abstract

“…Expression of Spi1 and Fli1 can be activated by position speci®c integration of the Friend murine leukemia virus in murine erythroleukemias (BenDavid et al, 1991). Also, ETS1, ETS2 and ERG regulate the expression of metalloproteinase genes, such as stromelysin and collagenase (Buttice and Kurkinen, 1993;Buttice et al, 1996;Wasylyk et al, 1991), which are important for extracellular matrix degradation concomitant with tumor vascularization (angiogenesis) and metastasis.…”

Section: Introductionmentioning

confidence: 99%

A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13–15, a region subject to LOH and rearrangement in human carcinoma cell lines

Zhou

Tymms

et al. 1998

Oncogene

View full text Add to dashboard Cite

The ETS transcription factors are a large family implicated in the control of cellular proliferation and tumorigenesis. In addition, chromosomal translocations involving ETS family members are associated with a range of di erent human cancers. Given the extensive involvement of ETS factors in tumorigenesis, it becomes important to identify any additional ETS genes that may also play oncogenic roles. We identify a novel gene, ELF5, that appears to belong to the ELF (E74-likefactor) subfamily of the ETS transcription factor family, based upon similarity within the`ETS domain'. ELF5 displays a similar, but more restricted, expression pattern to that of the newly isolated epithelium-speci®c ETS gene, ELF3. Unlike most other ETS family members, ELF5 is not expressed in hematopoietic compartments, but is restricted to organs such as lung, stomach, kidney, prostate, bladder and mammary gland. ELF5 is localized to human chromosome 11p13 ± 15, a region that frequently undergoes loss of heterozygosity (LOH) in several types of carcinoma, including those of breast, kidney and prostate. We ®nd that ELF5 expression is not detectable in a number of carcinoma cell lines, some of which display loss or rearrangement of an ELF5 allele. Similar to other ETS family members, ELF5 displays speci®c binding to DNA sequences containing a GGAA-core. In addition, ELF5 is able to transactivate through these ETS sequences, present upstream from a minimal promoter. Our data suggest that ELF5 may play roles in mammary, lung, prostate and/or kidney function, and possibly also in tumorigenesis.

show abstract

Erg, an Ets-FamiEly member, differentially regulates human collagenase1 (MMP1) and stromelysin1 (MMP3) gene expression by physically interacting with the Fos/Jun complex

Cited by 86 publications

References 0 publications

Multiple changes in gene expression in chronic human Achilles tendinopathy

Multiple changes in gene expression in chronic human Achilles tendinopathy

Molecular phylogeny of the ETS gene family

A novel transcription factor, ELF5, belongs to the ELF subfamily of ETS genes and maps to human chromosome 11p13–15, a region subject to LOH and rearrangement in human carcinoma cell lines

Contact Info

Product

Resources

About