ERG–SOX4 interaction promotes epithelial–mesenchymal transition in prostate cancer cells

Wang, Lin; Li, Yanjiang; Yang, Xiaoqing; Yuan, Huipin; Li, Xiangzhi; Qi, Mei; Chang, Y W Y; Wang, Chunni; Fu, Weiwei; Yang, Muyi; Zhang, Juan; Han, Bo

doi:10.1002/pros.22783

Cited by 52 publications

(48 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SOX4 was reported to be upregulated in leiomyoma microarray studies previously 16,18,19 . Increased SOX4 gene expression was also reported in prostate, endometrial, colon, breast, and gastric cancer, as well as in leukemia [20][21][22][23] . SOX4 encodes a member of the SOX family of transcription factors that is involved in the regulation of embryonic development.…”

Section: Discussionmentioning

confidence: 86%

Gene expression analysis of uterine smooth muscle cells exposed to bisphenol A

Kang

Song

Cho

2014

Toxicol. Environ. Health Sci.

View full text Add to dashboard Cite

To evaluate the effect of bisphenol A (BPA) on gene expression changes in uterine myometrium, uterine smooth muscle cells were incubated with 2.28 ng/mL BPA for 48 h. Total RNA was extracted and quantified spectrophotometrically. Gene expression changes were analyzed using oligonucleotide microarray hybridization comparing the treated and control groups (1.5-fold change, p⁄ ⁄0.05). Data indicated that 560 genes were upregulated and 231 were downregulated in response to BPA exposure. The biological processes positively associated with gene upregulation included the regulation of cell differentiation, cellular metabolic processes, cell proliferation and smooth muscle contraction. In contrast, genes involved in mitosis, lipid metabolism, regulation of muscle cell differentiation and cell adhesion were significantly downregulated. In the KEGG pathway analysis, MAPK signaling pathway, insulin signaling pathway and extracellular matrix (ECM)-mediated interactions were positively associated with gene upregulation. Results Gene Expression Profiles Induced by BPA in Uterine Smooth Muscle CellGene expression changes were analyzed by comparing the treated and control groups using statistical criteria (1.5-fold change, p⁄0.05). Three independent samples were analyzed. Results indicated that 560 genes were upregulated in response to BPA exposure and 231 were downregulated.The 560 upregulated and 231 downregulated genes were classified according to the GO terms of biological processes and KEGG pathway analysis, to investigate the molecular mechanisms related to BPA exposure. EASE analysis (http://david.abcc.ncifcrf.gov/) was also performed. The key biological processes that were significantly affected (Fisher's exact test, p⁄

show abstract

Section: Discussionmentioning

confidence: 86%

Gene expression analysis of uterine smooth muscle cells exposed to bisphenol A

Kang

Song

Cho

2014

Toxicol. Environ. Health Sci.

View full text Add to dashboard Cite

show abstract

“…This is suggested by multiple lines of evidence. First, its expression was significantly elevated in multiple human cancers, including tumors of the lung, breast and prostate [16,17,18]. Second, its overexpression was closely correlated with tumor progression and metastasis [19,20].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Luο

Tang

Gao

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Multiple MicroRNAs (miRNAs) have been identified in the development and progression of osteosarcoma. However, the expression and roles of miR-212 in osteosarcoma remain largely undefined. Methods: Real-time PCR assays were used to detect the expression of miR-212 in human osteosarcoma tissues. MiR-212 mimics were introduced into MG63 and U2OS cells. Bioinformatic prediction was used to identify the potential targets of miR-212. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-212. Results: miR-212 was significantly down-regulated in human osteosarcoma tissues, compared with adjacent normal tissues. Introduction of miR-212 mimics into MG63 and U2OS cells inhibited cell proliferation and invasion. Besides, miR-212 overexpression could also inhibit tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the sex-determining region Y-box 4 (Sox4) is a target gene of miR-212. Sox4 inhibition phenocopied the roles of miR-212, while restored expression of Sox4 dampened miR-212-mediated suppression of tumor progression. Conclusion: The miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.

show abstract

“…ETS + patients with particularly high expression of these genes may respond more favorably in future targeted therapy. ERG promotes SOX4 expression to stimulate epithelial to mesenchymal transition in PCa [78], and these mouse data suggest that ETV1 may promote SOX4 expression as a common component of ETS-driven oncogenesis.…”

Section: Discussionmentioning

confidence: 99%

Interaction of the Androgen Receptor, ETV1, and PTEN Pathways in Mouse Prostate Varies with Pathological Stage and Predicts Cancer Progression

et al. 2015

View full text Add to dashboard Cite

To examine the impact of common somatic mutations in prostate cancer (PCa) on androgen receptor (AR) signaling, mouse models were designed to perturb sequentially the AR, ETV1 and PTEN pathways. Mice with "humanized" AR (hAR) alleles that modified AR transcriptional strength by varying polyglutamine tract (Q-tract) length were crossed with mice expressing a prostate-specific, AR-responsive ETV1 transgene (ETV1Tg). While hAR allele did not grossly affect ETV1-induced neoplasia, ETV1 strongly antagonized global AR regulation and repressed critical androgen-induced differentiation and tumor suppressor genes, such as Nkx3-1 and Hoxb13. When Pten was varied to determine its impact on disease progression, mice lacking one Pten allele (Pten+/−) developed more frequent prostatic intraepithelial neoplasia (PIN). Yet only those with the ETV1 transgene progressed to invasive adenocarcinoma. Furthermore, progression was more frequent with the short Q-tract (stronger) AR, suggesting that the AR, ETV1 and PTEN pathways cooperate in aggressive disease. On the Pten+/− background, ETV1 had markedly less effect on AR target genes. However, a strong inflammatory gene expression signature, notably upregulation of Cxcl16, was induced by ETV1. Comparison of mouse and human patient data stratified by presence of ETS fusion genes highlighted additional factors, some not previously associated with prostate cancer but for which targeted therapies are in development for other diseases. In sum, concerted use of these mouse models illuminates the complex interplay of AR, ETV1 and PTEN pathways in pre-cancerous neoplasia and early tumorigenesis, disease stages difficult to analyze in man.

show abstract

ERG–SOX4 interaction promotes epithelial–mesenchymal transition in prostate cancer cells

Abstract: Our findings define a key role for ERG/SOX4 in the development of a subset of PCa and highlight the clinical importance of identifying molecularly defined tumor subgroups.

Cited by 52 publications

References 37 publications

Gene expression analysis of uterine smooth muscle cells exposed to bisphenol A

Gene expression analysis of uterine smooth muscle cells exposed to bisphenol A

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Interaction of the Androgen Receptor, ETV1, and PTEN Pathways in Mouse Prostate Varies with Pathological Stage and Predicts Cancer Progression

Contact Info

Product

Resources

About