Pavlov [Adv. Math.295 (2016), 250–270; Nonlinearity32 (2019), 2441–2466] studied the measures of maximal entropy for dynamical systems with weak versions of specification property and found the existence of intrinsic ergodicity would be influenced by the assumptions of the gap functions. Inspired by these, in this article, we study the dynamical systems with non-uniform specification property. We give some basic properties these systems have and give an assumption for the gap functions to ensure the systems have the following five properties: CO-measures are dense in invariant measures; for every non-empty compact connected subset of invariant measures, its saturated set is dense in the total space; ergodic measures are residual in invariant measures; ergodic measures are connected; and entropy-dense. In addition, we will give examples to show the assumption is optimal.