Background Nurses’ physical and mental well-being is crucial for prompt patient-care service. While research has been conducted on the specific causes of musculoskeletal disorders (MSDs) and occupational stress among Indian nurses, a comprehensive picture considering all these variables is still lacking. This study investigated MSD prevalence and psychosocial stress levels among female nurses in West Bengal, India, and the connections between these factors and MSDs. Methods The study was performed in nine different hospitals and nursing homes in West Bengal, India. The modified Nordic questionnaire and Copenhagen Psychosocial Questionnaire (COPSOQ) were used. The body height, body weight, BMI, pulse rate and blood pressure of the subjects were measured using an anthropometer, a weighing machine and a digital blood pressure monitor, respectively. Results 80.9% of subjects reported that they had discomfort in the past 12 months, with the highest percentage (52.1%) of complaints of low-back pain. From COPSOQ, concerning mean values were found in the demand scales and general health. The presence of different discomforts was found to be significantly related to various scales of COPSOQ. Conclusion Increased cognitive, quantitative and sensorial demands were found to be major contributors to the high prevalence of MSDs among nurses in this study. As the well-being of nurses is pivotal for maintaining well-ordered health systems, regular follow-ups on their physical and psychological health are necessary.