Ergostane-Type Sterols from King Trumpet Mushroom (Pleurotus eryngii) and Their Inhibitory Effects on Aromatase

Kikuchi, Takashi; Motoyashiki, Naoki; Yamada, Takeshi; Shibatani, Kanae; Ninomiya, Kiyofumi; Morikawa, Toshio; Tanaka, Reiko

doi:10.3390/ijms18112479

Cited by 14 publications

(11 citation statements)

References 30 publications

(31 reference statements)

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“…Some examples are polysaccharides, glycoproteins, proteoglycans, proteins, quinones, cerebrosides, isoflavones, catechols, triacylglycerols, phenolic acids, terpenes, and steroids (Ferreira et al, 2010;Villares et al, 2012). Evidences support the theory of antitumoral properties of sterols, including inhibition of tumor growth and stimulation of apoptosis (Kikuchi et al, 2017). According to Barreira and Ferreira (2015), the mechanism underlying the inhibition of cell growth could be similar to the mechanism associated with protein kinase C activity.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

Finimundy

Abreu

Bonetto

et al. 2018

Food and Chemical Toxicology

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Pleurotus sajor-caju (PSC) is an edible mushroom used in food supplements, presenting antitumor properties through induction of cell death pathways. The PSC potential against colorectal cancer was analyzed by exposing HCT116 cells to different PSC extracts. The PSC n-hexane extract (PSC-hex) showed the highest cytotoxicity effect (IC value 0.05 mg/mL). The observed cytotoxicity was then associated to apoptosis-promoting and cell cycle-arrest pathways. PSC-hex was able to induce apoptosis related to breakdown of mitochondrial membrane potential and ROS generation. The absence of cytotoxicity in HTC116 and HTC116 cells, alongside with an increase in p53, Bax and Caspase-3 expression, and decrease in Bcl-2 expression, supports that the pro-apoptotic effect is probably induced through a p53 associated pathway. PSC-hex induced cell cycle arrest at G2/M in HCT116 without cytotoxicity in HTC116 cells. These findings suggest that a p21/p53 cell cycle regulation pathway is probably disrupted by compounds present on PSC-hex. Identification of the major components was then performed with ergosta-5,7,22-trien-3β-ol representing 30.6% of total weight. In silico docking studies of ergosta-5,7,22-trien-3β against Bcl-2 were performed and results show a credible interaction with the Bcl-2 hydrophobic cleft. The results show that PSC-hex can be used as supplementary food for adjuvant therapy in colorectal carcinoma.

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Section: Introductionmentioning

confidence: 99%

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

Finimundy

Abreu

Bonetto

et al. 2018

Food and Chemical Toxicology

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“…The ergosterol content in P. eryngii was reported as 20 mg/100 g dw, although a higher value was measured in commercial samples (Souilem et al 2017). Kikuchi et al (2017Kikuchi et al ( , 2018 reported the isolation and structure elucidation of ergostane type sterols and bisabolane-type sesquiterpenes from P. eryngii with aromatase and nitric oxide production inhibitory effects, respectively (Table 2). Wang et al (2013a) reported the identification of novel and rare perhydrobenzannulated 5,5-spiroketal sesquiterpenes, named pleurospiroketals A-E from the edible mushroom P. cornucopiae with inhibitory activity against nitric oxide production in lipopolysaccharide-activated macrophages with IC 50 values between 6.8-20.8 lM.…”

Section: Triterpenoids and Sterolsmentioning

confidence: 99%

“…Many sterols such as campesterol, lanosterol, brassicasterol, b-sitosterol, ergosterol were analyzed in the fruiting bodies of different Tuber species Pleurotus ostreatus Ergosterol, ergosta-5,7-dienol, ergosta-7-enol, ergosta-7,22-dienol, oleanolic acid, ursolic acid Sarma et al (2018) and Koutrotsios et al (2017) Pleurotus eryngii Ergosterol Souilem et al (2017) Ergostane-type sterols Kikuchi et al (2017) Strophasterols E and F Kikuchi et al (2019) ( Table 2). The main sterols found in Tuber magnatum Picco and T. melanosporum fruiting bodies were ergosterol and brassicasterol, which amounted to 63.1-66.7% and 15.7-21.3% of the total sterols, respectively.…”

Section: Triterpenoids and Sterolsmentioning

confidence: 99%

Mycochemicals in wild and cultivated mushrooms: nutrition and health

et al. 2021

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The mushrooms have contributed to the development of active ingredients of fundamental importance in the field of pharmaceutical chemistry as well as of important tools in human and animal health, nutrition, and functional food. This review considers studies on the beneficial effects of medicinal mushrooms on the nutrition and health of humans and farm animals. An overview of the chemical structure and composition of mycochemicals is presented in this review with particular reference to phenolic compounds, triterpenoids and sterols, fatty acids and lipids, polysaccharides, proteins, peptides, and lectins. The nutritional value and chemical composition of wild and cultivated mushrooms in Italy is also the subject of this review which also deals with mushrooms as nutraceuticals and the use of mushrooms in functional foods. The nutraceutical benefits of UV irradiation of cultivated species of basidiomycetes to generate high amounts of vitamin D2 is also highlighted and the ability of the muhsrooms to inhibit glycation is analyzed. Finally, attention is paid to studies on bioactivities of some Italian wild and cultivated mushrooms with particular reference to species belonging to the genus Pleurotus. The review highlights the potential of medicinal mushrooms in the production of mycochemicals that represent a source of drugs, nutraceutical, and functional food. Graphic abstract

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“…Many studies have shown that mushrooms have different biological activities. In studies on mushrooms, it has been reported to have many activities such as antioxidant, antitumor, antimicrobial, antiaromatase activity, anti-inflammatory activity, antiproliferative activity and immunomodulatory activity (Akgul et al, 2017;Bal et al, 2017;Kikuchi et al, 2017;Sriramulu and Sumathi, 2017;Wang et al, 2018;Sevindik 2018a;Gürgen et al, 2020;Mushtaq et al, 2020). Turkey has a high diversity in terms of biodiversity.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Element Content of Suillus collinitus

Bal

2021

Turkish JAF Sci.Tech.

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Mushrooms used as food have medicinal importance due to their antioxidant compounds. In this context, it is very important to determine the biological potential of fungi and to reveal these medicinal properties. In this study, it was aimed to determine the element contents, total antioxidant status, total oxidant status, oxidative stress index of Suillus collinitus (Fr.) Kuntze mushroom. In this context, the mushroom samples were extracted with ethanol in the Soxhlet extractor. Element contents were determined using atomic absorption spectrometry. Total antioxidant (TAS) and total oxidant (TOS) levels and oxidative stress index (OSI) were determined using Rel Assay commercial kits. As a result of the study, it was determined that the TAS value of S. collinitus was 2.467 ± 0.145 mmol/L, TOS value was 17.845 ± 0.273 µmol/L and OSI value was 0.677 ± 0.030. In addition, the Fe content (350.72 ± 10.23), Cu content (68.11 ± 2.51), Pb content (11.58 ± 2.43), Zn content (10.46 ± 1.28) and Ni content (1.47 ± 0.21 mg.kg-1) of S. collinitus measured. As a result, S. collinitus mushroom is thought to be a natural source of antioxidants. It has also been observed that the element contents are at normal levels.

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Ergostane-Type Sterols from King Trumpet Mushroom (Pleurotus eryngii) and Their Inhibitory Effects on Aromatase

Cited by 14 publications

References 30 publications

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

Mycochemicals in wild and cultivated mushrooms: nutrition and health

Antioxidant Activity and Element Content of Suillus collinitus

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