Ergosterol peroxide inhibits ovarian cancer cell growth through multiple pathways

Tan, Weiwei; Pan, Meihong; Liu, Hui; Tian, Hequn; Ye, Qing; Liu, Hongda

doi:10.2147/ott.s139009

Cited by 48 publications

(41 citation statements)

References 31 publications

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“…Total mRNA was isolated from fresh-frozen specimens using Trizol reagent (Invitrogen, Carlsbad, USA), following a standard protocol [ 19 ]. RNA was reversely transcribed into cDNA by using the Primer-Script RT Enzyme Mix (Invitrogen, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Increased Expression of Lysine-Specific Demethylase 5B (KDM5B) Promotes Tumor Cell Growth in Hep3B Cells and is an Independent Prognostic Factor in Patients with Hepatocellular Carcinoma

Gong

Yan

et al. 2018

Med Sci Monit

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BackgroundLysine-specific demethylase 5B (KDM5B) is overexpressed in several types of cancer. However, the clinical significance of KDM5B expression in hepatocellular carcinoma (HCC) remains unclear. The aims of the present study were to examine the functional effects of KDM5B in the Hep3B cell line, the expression levels of KDM5B in human HCC tissues, and the association between KDM5B expression and clinical outcome in patients with HCC.Material/MethodsImmunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to examine the expression levels of KDM5B in HCC tissues and adjacent normal liver tissues. In the HCC cell line, Hep3B, the effects of KDM5B on cell proliferation and migration, and KDM5B small interfering RNA (siRNA) were used to study KDM5B knockdown. Univariate and multivariate analysis assessed the prognostic role of KDM5B in HCC patients. Kaplan-Meier analysis and the log-rank test evaluated clinical outcomes.ResultsIn the HCC cell line, Hep3B, KDM5B expression promoted promote tumor cell proliferation and colony formation. Increased expression of KDM5B in HCC tissues, compared with adjacent normal liver tissues, and was associated with larger tumor size, advanced TNM stage, and reduced overall survival in patients with HCC. Multivariate analysis identified KDM5B expression as an independent prognostic factor.ConclusionsIncreased expression of KDM5B was significantly correlated with poorer prognosis in patients with patients with HCC, indicating the possible potential of KDM5B as a novel clinical biomarker and therapeutic target.

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Section: Methodsmentioning

confidence: 99%

Increased Expression of Lysine-Specific Demethylase 5B (KDM5B) Promotes Tumor Cell Growth in Hep3B Cells and is an Independent Prognostic Factor in Patients with Hepatocellular Carcinoma

Gong

Yan

et al. 2018

Med Sci Monit

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“…Lupenone (2) was found in the first time from the plant E. tirucalli growing in China 14 since 2011 but it could be found in many higher plants belonging to the Euphorbia genus. Ferulic acid (5) 17,18 , to the best of our knowledge, two compounds 3 and 4 were isolated from this species for the first time.…”

Section: Resultsmentioning

confidence: 99%

A further investigation on the chemical constituents from Euphorbia tirucalli growing in Binh Thuan province

Duong

2019

Sci. Tech. Dev. J.

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Introduction: Euphorbia tirucalli L. is a medicinal plant popularly distributed in Asian countries. In Vietnam, only one study on the polar extract the plant Euphorbia tirucalli growing in Binh Thuan province, Vietnam was reported, revealing several phenolic components. As of 2019, no chemical reports on the non-polar extract from the Vietnamese plant were found. This research described the isolation and elucidation of compounds isolated from the non-polar extract of E. tirucalli growing in Binh Thuan province. Method: The n-hexane extract of this plant was carried out by using normal phase silica gel column chromatography, thin-layer chromatography, and gel chromatography . Analysis of spectroscopic data and a comparison of the NMR data with that in the literature led to the structural elucidation of isolated compounds. Results: Three terpenoid compounds, euphol (1), lupenone (2), and vomifoliol (3), along with ergosterol peroxide (4), ferulic acid (5), and vanillic acid (6) were isolated and elucidated. Conclusions: Among them, compound 3 and 4 were reported in the first time from E. tirucalli.

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“…Finally, real-time Q-PCR was performed to compare the expressions of EphA8 in OTSCC tissues and adjacent non-tumor tissues. GAPDH was chosen as an internal reference gene [ 22 ]. The primer sequences are as followed: EphA8 forward, 5′-CCACCAGGGTATGTAAATATC-3′; EphA8 reverse, 5′-TGTGCTTTGAAGACCATTT-3′; GAPDH forward, 5′-AGGGCTGCTTTTAACTCTGGT-3′; GAPDH reverse, 5′-CCCCACTTGATTTTGGAGGGA-3′.…”

Section: Methodsmentioning

confidence: 99%

EphA8 is a Prognostic Factor for Oral Tongue Squamous Cell Carcinoma

Liu

2018

Med Sci Monit

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BackgroundOral tongue squamous cell carcinoma (OTSCC) is the most common malignancy of the oral cavity. Here we explore the potential effects of EphA8, which is one of the receptors in Ephs subfamily of RTKs (receptor tyrosine kinases), in the progression and prognosis of OTSCC.Material/MethodsA total of 119 OTSCC patients were enrolled in this retrospective study. Immunohistochemistry (IHC) staining and quantitative polymerase chain reaction (Q-PCR) were utilized to examine the expression of EphA8 in OTSSC tissues and adjacent non-tumor tissues. The relationship between EphA8 expression and the clinicopathological features of OTSCC patients were analyzed by chi-square. Survival analysis was carried out with Kaplan-Meier curve and the related log-rank test. Multivariate analysis was then undertaken to assess the prognosis factor by utilizing the Cox proportional hazard regression model. In addition, MTT assay and Matrigel invasion assay were performed to examine the effects of EphA8 on the proliferation and invasion capacities of human oral squamous carcinoma cells (SCC-25) and human tongue squamous cell carcinoma cells (H357).ResultsQ-PCR and IHC staining revealed that EphA8 was highly expressed in OTSCC tissues, especially in advanced stage OTSCC tissues. Kaplan-Meier curve showed that high EphA8 expression was significantly associated with poor prognosis, similar to age, smoking habit, drinking habit, tumor size, and TNM stage. Multivariate analysis indicated that EphA8 expression could serve as an independent prognostic factor in OTSCC. In vitro experiments revealed that overexpression of EphA8 might promote the progression of OTSCC via enhancing the invasion capacity but not proliferation capacity of tumor cells.ConclusionsEphA8 was highly expressed in OTSCC tissues and was significantly associated with poor prognosis of OTSCC.

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Ergosterol peroxide inhibits ovarian cancer cell growth through multiple pathways

Cited by 48 publications

References 31 publications

Increased Expression of Lysine-Specific Demethylase 5B (KDM5B) Promotes Tumor Cell Growth in Hep3B Cells and is an Independent Prognostic Factor in Patients with Hepatocellular Carcinoma

Increased Expression of Lysine-Specific Demethylase 5B (KDM5B) Promotes Tumor Cell Growth in Hep3B Cells and is an Independent Prognostic Factor in Patients with Hepatocellular Carcinoma

A further investigation on the chemical constituents from Euphorbia tirucalli growing in Binh Thuan province

EphA8 is a Prognostic Factor for Oral Tongue Squamous Cell Carcinoma

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