Ergot species of the Claviceps purpurea group from South Africa

Linde, Elna J. van der; Pešicová, Kamila; Pažoutová, Sylvie; Stodůlková, Eva; Flieger, Miroslav; Kolařík, Miroslav

doi:10.1016/j.funbio.2016.05.006

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…To confirm the identities of the strains, DNA sequences of partial elongation factor 1-α gene were extracted from each assembly of nine genomes using Geneious 10.0.9 (Kearse et al 2012); the fragments were aligned with representative sequences developed by Pažoutová et al (2015) and Van der Linde et al (2016) using MAFFT version 7 (https://mafft.cbrc.jp/alignment/server/) Katoh & Standley 2013. Maximum Parsimony (MP) analyses were performed using PAUP* 4.0b10 (Swofford 2002) with heuristic search of 200 replicates random stepwise addition, bootstrapping of 1000 replicates.…”

Section: Methodsmentioning

confidence: 99%

“…These groups were later identified as cryptic species based on multi-gene phylogenetic and population genetic analyses, i.e. C. arundinis , C. humidiphila , and C. spartinae (Douhan et al 2008, Pažoutová et al 2015) and recently a few more species from South Africa were described as a part of the species complex (Van der Linde et al 2016). In Canada, the incidence of ergot diseases in Alberta, Saskatchewan and Manitoba has been increasing since 2002 (Menzies & Turkington 2014).…”

Section: Introductionmentioning

confidence: 99%

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Nine draft genome sequences of Claviceps purpurea s.lat., including C. arundinis, C. humidiphila, and C. cf. spartinae, pseudomolecules for the pitch canker pathogen Fusarium circinatum, draft genome of Davidsoniella eucalypti, Grosmannia galeiformis, Quambalaria eucalypti, and Teratosphaeria destructans

et al. 2018

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This genome announcement includes draft genomes from Claviceps purpurea s.lat., including C. arundinis , C. humidiphila and C. cf. spartinae . The draft genomes of Davidsoniella eucalypti, Quambalaria eucalypti and Teratosphaeria destructans, all three important eucalyptus pathogens, are presented. The insect associate Grosmannia galeiformis is also described. The pine pathogen genome of Fusarium circinatum has been assembled into pseudomolecules, based on additional sequence data and by harnessing the known synteny within the Fusarium fujikuroi species complex. This new assembly of the F. circinatum genome provides 12 pseudomolecules that correspond to the haploid chromosome number of F. circinatum . These are comparable to other chromosomal assemblies within the FFSC and will enable more robust genomic comparisons within this species complex.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nine draft genome sequences of Claviceps purpurea s.lat., including C. arundinis, C. humidiphila, and C. cf. spartinae, pseudomolecules for the pitch canker pathogen Fusarium circinatum, draft genome of Davidsoniella eucalypti, Grosmannia galeiformis, Quambalaria eucalypti, and Teratosphaeria destructans

et al. 2018

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“…Within C. sect. Claviceps we observed support for the separation of recently described species from C. purpurea s.s. (Fig.1) (Pažoutová et al 2000, 2002, 2015; Douhan et al 2008; van der Linde et al 2016; Shoukouhi et al 2019; Liu, M et al Submitted ). Overall, C. sect.…”

Section: Resultsmentioning

confidence: 98%

Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Wyka

Mondo

Liu

et al. 2020

Preprint

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1 2 Genus-wide comparison reveals divergence and evolution of the four sections within the 3 genus Claviceps are the result of varying mechanisms driving genome evolution and host 4 range 5 6 Abstract: 16 Background: 17 The genus Claviceps has been known for centuries as an economically important fungal 18 genera for pharmacology and agricultural research. Despite this little is known about its 19 evolution. Only recently have researchers begun to unravel the evolutionary history of the genus, 20 with origins in South America and classification of four distinct sections through ecological, 21 morphological, and metabolic features (Claviceps sects. Citrinae, Paspalorum, Pusillae, and 22 Claviceps). The first three sections are additionally characterized by narrow host range, while 23 sect. Claviceps is considered evolutionarily more successful and adaptable as it has the largest 24 host range and biogeographical distribution. However, the reasons for this success and 25 adaptability remain unclear.26Results: 27 Our study elucidates these factors by sequencing and annotating 50 Claviceps genomes, 28 representing 21 species, for a comprehensive comparison of genome architecture and plasticity 29 in relation to host range potential. Our results show the trajectory from specialized one-speed 30 genomes (C. sects. Citrinae and Paspalorum) to adaptive two-speed genomes (C. sects. Pusillae 31 and Claviceps) through co-localization of TEs around predicted effectors and loss of RIP 32 resulting in unconstrained tandem gene duplication coinciding with increased host range 33 potential and speciation.34 Conclusions: 35 Alterations of genomic architecture and plasticity can substantially influence and shape 36 the evolutionary trajectory of fungal pathogens and their adaptability. Furthermore, our study 37 provides a large increase in available genomic resources to propel future studies of Claviceps in 38 pg. 3 pharmacology and agricultural research, as well as, research into deeper understanding of the 39 evolution of adaptable plant pathogens. 40 41

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“…Populations of others, i.e., Paleartic C. nigricans ( Eleocharis, Scirpus ) or South African Claviceps capensis ( Ehrharta ), C. fimbristylidis ( Fimbristylis ), C . pazoutovae ( Ehrharta and Stipa ), C. macroura ( Cenchrus ), or C. monticola ( Brachypodium ) are rare in abundance and their hosts have low palatability [34,35].…”

Section: Discussionmentioning

confidence: 99%

“…(USA, New Zealand). Materials originated from previous studies [2,34,63] or were collected during the course of this study (Table 1, Table S1). Sclerotia were identified using the ITS rDNA sequence barcode.…”

Section: Methodsmentioning

confidence: 99%

Ergochromes: Heretofore Neglected Side of Ergot Toxicity

Flieger

Stodůlková

Wyka

et al. 2019

Toxins

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Ergot, fungal genus Claviceps, are worldwide distributed grass pathogens known for their production of toxic ergot alkaloids (EAs) and the great agricultural impact they have on both cereal crop and farm animal production. EAs are traditionally considered as the only factor responsible for ergot toxicity. Using broad sampling covering 13 ergot species infecting wild or agricultural grasses (including cereals) across Europe, USA, New Zealand, and South Africa we showed that the content of ergochrome pigments were comparable to the content of EAs in sclerotia. While secalonic acids A–C (SAs), the main ergot ergochromes (ECs), are well known toxins, our study is the first to address the question about their contribution to overall ergot toxicity. Based on our and published data, the importance of SAs in acute intoxication seems to be negligible, but the effect of chronic exposure needs to be evaluated. Nevertheless, they have biological activities at doses corresponding to quantities found in natural conditions. Our study highlights the need for a re-evaluation of ergot toxicity mechanisms and further studies of SAs’ impact on livestock production and food safety.

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Ergot species of the Claviceps purpurea group from South Africa

Cited by 10 publications

References 31 publications

Whole genome comparisons of ergot fungi reveals the divergence and evolution of species within the genus Claviceps are the result of varying mechanisms driving genome evolution and host range expansion

Ergochromes: Heretofore Neglected Side of Ergot Toxicity

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