ERIC-PCR-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems

Wang, L.; Jin, Yongfeng; Zhao, Liping; Pang, Xiaoyan; Zhang, Xiaojun

doi:10.1111/j.1472-765x.2009.02696.x

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…Wang et al . () and Yuan et al . () reported the bioremediation of aromatic hydrocarbons, such as phenol, phenanthrene and pyrene under high enantiomeric excess with A. nicotianae .…”

Section: Resultsmentioning

confidence: 89%

“…The previous studies on A. nicotianae mainly focused on effect of metal ions, such as copper (Nakajima 2002), uranyl ion (Takehiko 2002), lithium (Takehiko 2005), and cadmium (Tsuruta et al 2014). Wang et al (2009) and Yuan et al (2009) reported the bioremediation of aromatic hydrocarbons, such as phenol, phenanthrene and pyrene under high enantiomeric excess with A. nicotianae. However, this study is the first to report that A. nicotianae degrades organochlorine pesticides.…”

Section: Biodegradation Of Other Organochlorine Pesticides By Strain mentioning

confidence: 99%

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Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19

Wang

et al. 2015

Lett Appl Microbiol

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The degradation of pentachloronitrobenzene (PCNB) by an individual bacterial strain is being reported for the first time. The efficient PCNB-degrading strain DH19 was isolated from ginseng rhizosphere soil and identified as Arthrobacter nicotianae. The strain could utilize PCNB as the sole carbon source for growth and degradation. In addition, strain DH19 could efficiently degrade dichlorodiphenyl trichloroethane, hexachlorocyclohexane, cypermethrin and cyhalothrin. Five metabolites from PCNB degradation were identified, and a possible degradation pathway was deduced. The results suggest that A. nicotianae DH19 has great potential for the bioremediation of PCNB-contaminated environments.

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“…Wang et al . () and Yuan et al . () reported the bioremediation of aromatic hydrocarbons, such as phenol, phenanthrene and pyrene under high enantiomeric excess with A. nicotianae .…”

Section: Resultsmentioning

confidence: 89%

Section: Biodegradation Of Other Organochlorine Pesticides By Strain mentioning

confidence: 99%

Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19

Wang

et al. 2015

Lett Appl Microbiol

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“…The genetic diversity of Paenibacillus populations in the rhizosphere of wheat has been reported by using ERIC-PCR and the fingerprints were highly reproducible (Guemouri-Athmani et al, 2000). The use of ERIC-PCR for rapid and reliable detection of Arthrobacter has been reported (Wang et al, 2009). RAPD-PCR has been successfully used for genetic fingerprinting and molecular typing for many microorganisms (Gurtler et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

DEVELOPMENT OF DNA MARKERS FOR DETECTION OF INOCULATED BACTERIA IN THE RHIZOSPHERE OF WHEAT (Triticum aestivum L.)

Basheer¹,

Zaheer²,

Qaisrani³

et al. 2016

PAKJAS

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Plant growth-promoting rhizobacteria (PGPR) are a group of soil microorganisms that improve plant growth and yield through a number of diverse mechanisms such as phosphate solubilization, nitrogen fixation, production of phytohormones, and repression of soil borne pathogens. Due to high cost of chemical fertilizers and negative environmental effects, the use of PGPR as biofertilizer is advantageous for development of sustainable agriculture. The objective of this study was to develop DNA-based markers for five PGPR strains to detect these bacteria in the rhizosphere of inoculated wheat. The rhizobacterial strains included four phosphate solubilizer strains (Arthrobacter strain WP-2, Bacillus strain MP5, Rhodococcus strain M28 and Serratia strain 5D) and one phytohormone producer Azospirillum strain WS1. DNA-based markers were developed using 16S rRNA gene restriction patterns, Random Amplified Polymorphic DNA-PCR (RAPD-PCR), Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and BOX-PCR. In this study, the most differentiating DNA patterns for all strains were obtained by using the BOX primer in PCR. All the strains tested as single-strain inocula resulted in improved growth of wheat plants. The inoculated strains were successfully re-isolated from wheat rhizosphere and confirmed by BOXbased DNA markers. These results indicated that BOX-PCR is a useful and highly discriminatory fingerprinting technique for rapid detection of bacterial strains.

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“…A number of useful polymorphic techniques, such as RAPD, repetitive-sequence-based PCR (rep-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), and amplified fragment length polymorphism (AFLP), have been used for the detection of organism-specific distinctive DNA sequences. [28][29][30][31][32][33][34] Although only a few reports describe specific genetic detection methods applicable at the strain level, Fujimoto et al, 35) Maruo et al, 36) and Tilsala-Timisjärvi & Alatossava 37) reported the use of RAPD for strain-specific detection of Lactococcus lactis ssp. cremoris FC, Lactobacillus casei Shirota, and Lactobacillus rhamnosus Lc 1/3, respectively.…”

Section: )mentioning

confidence: 99%

Development of Strain-Specific PCR Primers for Quantitative Detection of <i>Bacillus mesentericus</i> Strain TO-A in Human Feces

Sato

Seo

Benno³

2014

Biological & Pharmaceutical Bulletin

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Strain-specific polymerase chain reaction (PCR) primers for detection of Bacillus mesentericus strain TO-A (BM TO-A) were developed. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. A 991-bp RAPD marker found to be strain-specific was sequenced, and two primer pairs specific to BM TO-A were constructed based on this sequence. In addition, we explored a more specific DNA region using inverse PCR, and designed a strain-specific primer set for use in real-time quantitative PCR (qPCR). These primer pairs were tested against 25 Bacillus subtilis strains and were found to be strain-specific. After examination of the detection limit and linearity of detection of BM TO-A in feces, the qPCR method and strain-specific primers were used to quantify BM TO-A in the feces of healthy volunteers who had ingested 3 10 8 colony forming unit (CFU) of BM TO-A per day in tablets. During the administration period, BM TO-A was detected in the feces of all 24 subjects, and the average number of BM TO-A detected using the culture method and qPCR was about 10 4.8 and 10 5.8 cells per gram of feces, respectively. Using the qPCR method, BM TO-A was detected in the feces of half of the subjects 3 d after withdrawal, and was detected in the feces of only one subject 1 week after withdrawal. These results suggest that the qPCR method using BM TO-A strain-specific primers is useful for the quantitative detection of this strain in feces.Key words Bacillus subtilis; strain-specific primer; quantitative polymerase chain reaction (PCR); human feces Probiotics are live microbial food supplements that beneficially affect the host by improving its microbial balance.

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ERIC-PCR-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems

Cited by 6 publications

References 22 publications

Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19

Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19

DEVELOPMENT OF DNA MARKERS FOR DETECTION OF INOCULATED BACTERIA IN THE RHIZOSPHERE OF WHEAT (Triticum aestivum L.)

Development of Strain-Specific PCR Primers for Quantitative Detection of <i>Bacillus mesentericus</i> Strain TO-A in Human Feces

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