ERK-independent African Green monkey pluripotent stem cells in a putative chimera-competent state

Angeles, Alejandro De Los; Elsworth, John D.; Redmond, D. Eugene

doi:10.1016/j.bbrc.2019.01.037

Cited by 7 publications

(5 citation statements)

References 42 publications

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“…The fact that humans and pigs are in many respects more similar than humans and mice, suggests that the xenobarrier between the two former species may prove easier to cross. Nevertheless, such an interspecies barrier does exist and this is why active research is currently being conducted between different species in order to elucidate its mechanisms and propose possible strategies to overcome it ( 68 - 71 ). Interestingly, it was recently reported that deletion of insulin-like growth factor 1 receptor (Igf1r) in mouse blastocysts significantly facilitates their colonization by both mouse and rat donor cells resulting in highly chimeric animals ( 72 ).…”

Section: Blastocyst Complementation: Challengesmentioning

confidence: 99%

In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges

Founta

Papanayotou

2022

Int J Stem Cells

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The ultimate goal of regenerative medicine is to replace damaged cells, tissues or whole organs, in order to restore their proper function. Stem cell related technologies promise to generate transplants from the patients’ own cells. Novel approaches such as blastocyst complementation combined with genome editing techniques open up new perspectives for organ replacement therapies. This review summarizes recent advances in the field and highlights the challenges that still remain to be addressed.

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Section: Blastocyst Complementation: Challengesmentioning

confidence: 99%

In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges

Founta

Papanayotou

2022

Int J Stem Cells

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“…Then, the cultivation medium and cultivation conditions were optimized (Gafni et al, 2013). Attempts have also been made to obtain pluripotent cells in the "naïve" status in primates (Fang et al, 2014;Chen Y. et al, 2015;De Los Angeles et al, 2019). Today, numerous studies are aimed at obtaining pluripotent ES cells in "naïve" status and maintaining this status under culture conditions (Liu et al, 2017;Kilens et al, 2018).…”

Section: Types Of "Chimera-competent" Cells For Injection Into a Blasmentioning

confidence: 99%

Generation of donor organs in chimeric animals via blastocyst complementation

2020

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The lack of organs for transplantation is an important problem in medicine today. The growth of organs in chimeric animals may be the solution of this. The proposed technology is the interspecific blastocyst complementation method in combination with genomic editing for obtaining “free niches” and pluripotent stem cell production methods. The CRISPR/Cas9 method allows the so-called “free niches” to be obtained for blastocyst complementation. The technologies of producing induced pluripotent stem cells give us the opportunity to obtain human donor cells capable of populating a “free niche”. Taken together, these technologies allow interspecific blastocyst complementation between humans and other animals, which makes it possible in the future to grow human organs for transplantations inside chimeric animals. However, in practice, in order to achieve successful interspecific blastocyst complementation, it is necessary to solve a number of problems: to improve methods for producing “chimeric competent” cells, to overcome specific interspecific barriers, to select compatible cell developmental stages for injection and the corresponding developmental stage of the host embryo, to prevent apoptosis of donor cells and to achieve effective proliferation of the human donor cells in the host animal. Also, it is very important to analyze the ethical aspects related to developing technologies of chimeric organisms with the participation of human cells. Today, many researchers are trying to solve these problems and also to establish new approaches in the creation of interspecific chimeric organisms in order to grow human organs for transplantation. In the present review we described the historical stages of the development of the blastocyst complementation method, examined in detail the technologies that underlie modern blastocyst complementation, and analyzed current progress that gives us the possibility to grow human organs in chimeric animals. We also considered the barriers and issues preventing the successful implementation of interspecific blastocyst complementation in practice, and discussed the further development of this method.

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“…An interesting question is what happens when both ERK and WNT signaling pathways are inhibited in human PS cells. Indeed, novel human and non‐human primate PS cells grown in the presence of ERK inhibitor PD0325901 and WNT inhibitor XAV939 have recently been reported (De Los Angeles et al., ; De Los Angeles, Elsworth, & Redmond, ). It will be of interest to determine whether these recently generated PS cells have different properties from previously reported naïve‐like stem cells.…”

Section: Introductionmentioning

confidence: 99%

The Pluripotency Continuum and Interspecies Chimeras

Angeles

2019

CP Stem Cell Biology

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Pluripotency refers to the capacity of single cells to form derivatives of the three germ layers-ectoderm, mesoderm, and endoderm. Pluripotency can be captured in vitro as a spectrum of pluripotent stem cell states stabilized in specialized laboratory conditions. The recent discovery that pluripotent stem cells can colonize the embryos of distantly related animal organisms could, with further refinement, enable the generation of chimeric embryos composed of cells of human and animal origin. If achievable, the production of humananimal chimeras will open up new opportunities for regenerative medicine, facilitating human disease modeling and human organ generation inside large animals. However, the generation of human-animal interspecies chimeras is anticipated to require human chimera-competent pluripotent stem cells. Thus, it remains imperative to examine the pluripotency continuum more closely in light of advances that will facilitate the production of human-animal chimeras. This piece will review the current understanding of the pluripotency continuum and interspecies chimeras. C 2019 by John Wiley & Sons, Inc.Keywords: chimeras r interspecies chimeras r naive pluripotent stem cells r pluripotent stem cells r primed pluripotent stem cells r formative pluripotency

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ERK-independent African Green monkey pluripotent stem cells in a putative chimera-competent state

Cited by 7 publications

References 42 publications

In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges

In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges

Generation of donor organs in chimeric animals via blastocyst complementation

The Pluripotency Continuum and Interspecies Chimeras

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