Abstract-Mining activities cause environmental pollutionproblems by contaminating nearby areas with heavy metals (HM) such as Cu, Fe, Zn and Al from overburden and leachates. HM can leach into groundwater, be transferred into rivers, and streams by surface run-off, in a form of dissolved species or as a part of suspended solids, culminating in their accumulation to toxic levels in the ecosystem downstream of the source. In view of such mobility attributed to bioavailability, the objective of this study was to assess the bioavailability and mobility of HM species in the sediments around a closed copper mine and subsequent to analysis with XRF, FTIR and a three-step sequential extraction process. Cu, Zn and Mn were predominantly bound to carbonates and could thus be easily desorbed during moisture laden periods, which will increase their bioavailability thus mobility, away from the source. The BCR fractions also showed that under oxidative reductive conditions, Fe species bound to Fe-Mn oxides, thus can be de-bonded in the presence of an acidified environment; therefore, be mobile for redistribute further downstream from the point of source and further contaminating pollutant free areas.