Erratum

Belfort, Alan M.; Belfort, Anne D.

doi:10.1007/bf02767511

Cited by 2 publications

(2 citation statements)

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“…Die Modifikation der OP-Technik führte zur Etablierung der „Hybridmethode“: Nach Unterbauchquerlaparotomie wird der Uterus externalisiert und es folgt der fetoskopische Verschluss der SBA. Vorteile dieser Methode sind die niedrigere Frühgeburtsrate, sowie Möglichkeit der vaginalen Geburt 3 .…”

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Etablierung des interdisziplinären Zentrums für die antenatale Therapie der Spina bifida aperta

Keil,

Sass,

Schulze

et al. 2023

Ultraschall in Der Medizin - European Journal of Ultrasound

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Etablierung des interdisziplinären Zentrums für die antenatale Therapie der Spina bifida aperta

Keil,

Sass,

Schulze

et al. 2023

Ultraschall in Der Medizin - European Journal of Ultrasound

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“…Plasmids encoding such a fusion protein can complement Escherichia coli thyA mutants if protein splicing occurs. In the absence of thymine, complementation of thyA allows growth and provides a method to select for protein splicing, but in the presence of thymine and trimethoprim, complementation leads to growth inhibition, thus allowing selection against protein splicing . Recently, Perler and colleagues described an assay for the inhibition of protein splicing mediated by the Mycobacterium xenopi GyrA intein, based on the conditional dominant lethality of quinolone-sensitive GyrA expressed in a quinolone- resistant E. coli host .…”

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confidence: 99%

An in Vitro Screening System for Protein Splicing Inhibitors Based on Green Fluorescent Protein as an Indicator

2003

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This paper describes an in vitro fluorometric assay system for protein splicing based on the RecA intein of Mycobacterium tuberculosis and a modified green fluorescent protein (GFP). The assay takes advantage of the fact that polypeptides inserted adjacent to residue 129 of GFP cause the protein to form inclusion bodies when expressed in Escherichia coli and to be incapable of fluorophore formation. However, when the inserted polypeptide is an intein, the renatured fusion protein can undergo protein splicing and chromophore formation. Comparison of chromophore formation by renatured GFP-intein fusion and renatured GFP showed that under optimal conditions (pH 6.5 and 20 degrees C) protein splicing is significantly slower than GFP chromophore formation. Taking advantage of the reversible inhibition of protein splicing by zinc ion, a fluorometric protein splicing assay was developed in which the denatured fusion protein of GFP and the RecA intein was purified on a metal ion affinity column and renatured in the presence of 2 mM ZnCl2. When diluted into appropriate buffers, protein splicing could be initiated by the addition of a molar excess of EDTA and followed fluorometrically. This assay should be valuable as a high-throughput screening system for protein splicing inhibitors as potential antimycobacterial agents and as tools for studying the mechanism of protein splicing.

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Erratum

Cited by 2 publications

References 0 publications

Etablierung des interdisziplinären Zentrums für die antenatale Therapie der Spina bifida aperta

Etablierung des interdisziplinären Zentrums für die antenatale Therapie der Spina bifida aperta

An in Vitro Screening System for Protein Splicing Inhibitors Based on Green Fluorescent Protein as an Indicator

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